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  4. Recombinant Human WW domain-containing oxidoreductase (WWOX)

Recombinant Human WW domain-containing oxidoreductase (WWOX)

  • 货号：
    CSB-YP873704HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP873704HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP873704HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP873704HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP873704HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WWOX
  • Uniprot No.：
  • 别名：
    5330426P09Rik; 9030416C10Rik; Aberrant WW domain-containing oxidoreductase; D16S432E; EC 1.1.1.-; EIEE28; FOR; FRA16D; Fragile site FRA16D oxidoreductase; Fragile site FRA16D Oxireductase; HHCMA56; MGC55975; PRO0128; Putative oxidoreductase; SCAR12; SDR41C1; Short chain dehydrogenase/reductase family 41C, member 1; WOX1; WW domain containing oxidoreductase; WW domain-containing oxidoreductase; WW domain-containing protein WWOX; wwox; WWOX_HUMAN; zgc:55975
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-414
  • 氨基酸序列
    MAALRYAGLD DTDSEDELPP GWEERTTKDG WVYYANHTEE KTQWEHPKTG KRKRVAGDLP YGWEQETDEN GQVFFVDHIN KRTTYLDPRL AFTVDDNPTK PTTRQRYDGS TTAMEILQGR DFTGKVVVVT GANSGIGFET AKSFALHGAH VILACRNMAR ASEAVSRILE EWHKAKVEAM TLDLALLRSV QHFAEAFKAK NVPLHVLVCN AATFALPWSL TKDGLETTFQ VNHLGHFYLV QLLQDVLCRS APARVIVVSS ESHRFTDIND SLGKLDFSRL SPTKNDYWAM LAYNRSKLCN ILFSNELHRR LSPRGVTSNA VHPGNMMYSN IHRSWWVYTL LFTLARPFTK SMQQGAATTV YCAAVPELEG LGGMYFNNCC RCMPSPEAQS EETARTLWAL SERLIQERLG SQSG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development. May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • 基因功能参考文献：
    1. We have identified a variant in WWOX and in lncRNA RP11-679B19.1 as a diseasemodifying genetic variant associated with recurrent fibrostenotic CD PMID: 29361163
    2. A model is supported in which FRA16D common fragile sites are sequences that may initiate replication in early-mid S phase but are slow to complete replication, and the chromosomal breaks and gaps observed in metaphase cells result from unreplicated DNA. PMID: 14603443
    3. Data conclude that WWOX sensitises EOC to paclitaxel via ER stress-induced apoptosis, and predicts clinical outcome in patients. Thus, ER stress response mechanisms could be targeted to overcome chemoresistance in cancer. PMID: 28749468
    4. Data show that patients with advanced-stage oral cancer were associated with a higher frequency of WWOX rs11545028 polymorphisms with the variant genotype TT than did patients with the wild-type gene. PMID: 27655721
    5. WWOX expression was strongly inhibited in human lung cancers and lung cancer cell lines. Reintroducing WWOX into lung cancer cells inhibited their invasive phenotype through downregulating RUNX2 and its target genes including MMP-9 expression. PMID: 27834355
    6. Our data suggests that the deletion genotypes of CNV-67048 in WWOX predispose their carriers to intracranial aneurysms PMID: 26910372
    7. It has been proposed that Fhit and Wwox loss work synergistically in cancer progression and that DNA damage caused by Fhit could be targeted early in cancer initiation for prevention, while DNA damage caused by Wwox loss could be targeted later in cancer progression, particularly in cancers that develop resistance to genotoxic therapies. (Review) PMID: 27773744
    8. genetic variations in WWOX may be a significant predictor of early HCC occurrence and a reliable biomarker for disease progression PMID: 28426730
    9. a WWOX-p53 network regulates normal bone formation and that disruption of this network in osteoprogenitors results in accelerated osteosarcoma. PMID: 27550453
    10. Low WWOX expression is associated with Head and Neck Squamous Cell Carcinoma. PMID: 27501229
    11. Knockdown of lung WWOX expression in mice was observed to cause neutrophil influx and was accompanied by a corresponding vascular leak and inflammatory cytokine production. In cultured human alveolar epithelial cells, loss of WWOX expression resulted in increased c-Jun- and IL-8-dependent neutrophil chemotaxis toward cell monolayers. PMID: 28283473
    12. a portion of ERK and WWOX relocates to the nucleus, suggesting their role in the induction of CD3 and CD8 expression in MOLT-4. PMID: 27339895
    13. Brca1-Wwox interaction supports non-homologous end-joining as the dominant DSB repair pathway in Wwox-sufficient cells PMID: 27869163
    14. Epigenetic mechanisms influence molecular patterns important for the bone-metastatic process, and this review highlights the role of WW-domain containing oxidoreductase (Wwox). [review] PMID: 28045433
    15. the deregulation of microRNA-214-3p expression is involved in a negative feedback loop with WWOX in osteosarcoma, indicating that miR-214-3p has potential basic and clinical implications. PMID: 27840941
    16. Functional analyses further showed a lower mRNA level of WWOX in tissues with the 0-copy or 1-copy than that in those with the 2-copy (P = 0.045). Our data suggested the CNV-67048 to be a risk factor of epithelia ovarian cancer in Chinese women. PMID: 27190995
    17. WW domain-containing oxidoreductase overexpression promoted apoptosis in 5637 cells and resulted in upregulation of Bax, downregulation of Bcl-2, and elevated levels of cleaved caspase-3 and cleaved PARP, indicating activation of the intrinsic apoptosis pathway. PMID: 27352332
    18. The SNP rs383362G >T of WWOX plays a role in chronic obstructive pulmonary disease inheritance. PMID: 26902998
    19. a novel SNP in the intron of the WW domain-containing oxidoreductase (WWOX) gene yielded significant replicated associations with skin tanning ability PMID: 26174610
    20. WWOX mutation is associated with early infantile epileptic encephalopathy. PMID: 26345274
    21. Our data suggest that the loss genotypes of CNV-67048 in WWOX predispose their carriers to chronic obstructive pulmonary disease. PMID: 25517572
    22. WWOX played roles in metabolic regulation and controlling central nervous system development. [review] PMID: 26499798
    23. Data suggest that Pc2- (polycomb repressive complex 2-) mediated SUMOylation of WWOX is essential for suppressive activity of WWOX in prostate cancer tumorigenesis. PMID: 26592150
    24. Compared to the controls, upregulation of WWOX and its Tyr33 phosphorylation was observed in the head region of all pterygium specimens. In the head and body of the pterygium specimens, WWOX expression was significantly higher than in the controls. PMID: 26120275
    25. Down regulation of WWOX is involved in tumorigenesis in intrahepatic cholangiocarcinoma. PMID: 25168293
    26. Decreased WWOX expression may interfere with gemcitabine sensitivity, and allele-specific binding at rs11644332 might be a causative molecular mechanism behind the clinically observed associations. PMID: 26857392
    27. Hypermethylation of WWOX gene is associated with Epstein-Barr virus-associated gastric carcinomas. PMID: 25720522
    28. The WWOX gene can downregulate the expression levels of cell cycle proteins cyclin E-CDK2 and cyclin D1-CDK4, which affects the cell cycle of ovarian cancer stem cells. PMID: 25891642
    29. The inhibition of the transcriptional activity of BCL9-2 by WWOX and HDAC3 constitutes a new molecular mechanism and provides new insight for a broad range of cancers. PMID: 25678599
    30. Results suggest that WWOX gene plays a role in the regulation of epithelial to mesenchymal transition process in endometrial cancer by controlling the expression of proteins associated with cell motility and suppressing mesenchymal markers. PMID: 25892250
    31. HGF and TGFbeta1 of bone-metastasis microenvironment acted co-ordinately, influencing non redundant pathways regulated by Twist program or Snail-transcription factor, with reversible MET switch PMID: 26041563
    32. miR-153 inhibited protein level of WWOX, a tumor suppressor and inhibitor of beta-catenin signaling, through targeting its 3'-untranslated region. PMID: 25708809
    33. WWOX inhibits prostate cancer progression through negatively regulating cyclin D1 in cell cycle lead to G1 arrest. PMID: 25659037
    34. overexpression of WWOX attenuated miR-134 induced H69 small cell lung cancer cell growth, and promoted cell apoptosis PMID: 26166818
    35. Study underscores a key role of allosteric communication in the ability of the WW2 orphan domain to chaperone physiological action of the WW1 domain within the context of the WW1-WW2 tandem module of WWOX. PMID: 25703206
    36. Inactivation of WWOX gene contributes to the progression of head and neck squamous cell carcinoma. PMID: 25612104
    37. A strong genetic cosegregation between the WWOX gene and the low HDL-C trait observed genetic associations at the chr16q23-24 locus. PMID: 24871327
    38. The role of WWOX in EHCC progression. PMID: 25502636
    39. WWOX undoubtedly incriminated as a gene for recessive infantile epileptic encephalopathy. PMID: 25411445
    40. There are roles of WWOX in the pathogenesis and endocrine therapy of breast cancer. PMID: 25476151
    41. WWOX structure gives insights into the functional versatility of this key signaling protein with important implications on its biology. PMID: 25662954
    42. Two and more tandem repeats cooperate in binding to their targets, in the well-characterized family of WWOX domains and their corresponding polyproline ligands. PMID: 25710931
    43. WWOX plays a role in the development, signaling pathway, prognosis, and treatment response of malignant glioma. PMID: 25432984
    44. WWOX is a member of a very interesting family of very large common fragile site genes and functions as a tumor suppressor involved in the development of many cancers. PMID: 25595185
    45. Aberrant WWOX expression contributes to the altered metabolism in cancer. PMID: 25595186
    46. WWOX can be regarded as a prognostic marker and a candidate molecule for targeted cancer therapies. PMID: 25595191
    47. WWOX plays a critical role in normal central nervous system development and disease. PMID: 25416187
    48. WWOX is a tumor suppressor gene and is associated with FRA16D locus fragility. PMID: 25538133
    49. WWOX has many functions, and its deletion affects cellular metabolism and neoplastic progression. PMID: 25491415
    50. WWOX gene is located in FRA16D, the highly affected chromosomal fragile site. PMID: 25681467

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  • 相关疾病：
    Esophageal cancer (ESCR); Spinocerebellar ataxia, autosomal recessive, 12 (SCAR12); Epileptic encephalopathy, early infantile, 28 (EIEE28)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus.
  • 蛋白家族：
    Short-chain dehydrogenases/reductases (SDR) family
  • 组织特异性：
    Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • 数据库链接：

    HGNC: 12799

    OMIM: 133239

    KEGG: hsa:51741

    STRING: 9606.ENSP00000457230

    UniGene: Hs.461453