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  4. Recombinant Human XIAP-associated factor 1 (XAF1)

Recombinant Human XIAP-associated factor 1 (XAF1)

  • 货号：
    CSB-YP764111HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP764111HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP764111HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP764111HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP764111HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    XAF1
  • Uniprot No.：
  • 别名：
    BIRC 4 binding protein; BIRC4 binding protein; BIRC4-binding protein; BIRC4BP; HSXIAPAF 1; HSXIAPAF1; XAF 1; XAF1; XAF1_HUMAN; XIAP associated factor 1; XIAP-associated factor 1; XIAPAF1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-301
  • 氨基酸序列
    MEGDFSVCRN CKRHVVSANF TLHEAYCLRF LVLCPECEEP VPKETMEEHC KLEHQQVGCT MCQQSMQKSS LEFHKANECQ ERPVECKFCK LDMQLSKLEL HESYCGSRTE LCQGCGQFIM HRMLAQHRDV CRSEQAQLGK GERISAPERE IYCHYCNQMI PENKYFHHMG KCCPDSEFKK HFPVGNPEIL PSSLPSQAAE NQTSTMEKDV RPKTRSINRF PLHSESSSKK APRSKNKTLD PLLMSEPKPR TSSPRGDKAA YDILRRCSQC GILLPLPILN QHQEKCRWLA SSKGKQVRNF S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
  • 基因功能参考文献：
    1. this study uncovers an important role for XAF1-MT2A antagonism as a linchpin to govern cell fate under various stressful conditions including heavy metal exposure. PMID: 28507149
    2. XAF1 expression was markedly altered in non-small cell lung cancer tumor samples when compared to that found in normal lung tissues. PMID: 28560416
    3. XAF1 expression was associated with VM in ovarian cancer, suggesting a potential role of XAF1 in the formation of VM in EOC. PMID: 28534973
    4. XAF1 may be a candidate tumor suppressor in neuroblastoma PMID: 27097110
    5. Dysfunction of XAF1 is frequent and is regulated through XAF1 promoter hypermethylation in gastric cancer. Circulating methylated XAF1 DNA was associated with tumor burden and malignant progression, which may be a valuable biomarker for diagnosis of gastric cancer, predicting patients' prognosis and monitoring tumor recurrence after surgery treatment. PMID: 23826230
    6. XAF1 promoter hypomethylation is associated with high-grade gliomas. PMID: 28122345
    7. human genome-wide gene expression profile assay was used to screen the targets of miR-3131. The overexpressed miR-3131 could lead to a significant decrease of DTHD1 and XAF1 mRNA level. PMID: 28034876
    8. XAF1 acts as a feedback regulator of the TNF receptor signaling pathway to suppress NF-kappaB activation PMID: 27768232
    9. Taken together, our results suggest that ROS generation might be a predisposing event in berberine-induced mitochondrial apoptosis in EBV-transformed B cells through the upregulation of XAF1 and GADD45alpha expression by MAPK and functional p53. PMID: 27121748
    10. Expression of XAF1 and TAp73 was also upregulated in casticin-treated T24 cells. PMID: 27349281
    11. Data show that X-linked inhibitor of apoptosis protein (XIAP) mRNA and protein expression increased, whereas XIAP-associated factor 1 (XAF1) mRNA and protein expression decreased with the growth of radiation dose and exposure time. PMID: 27048285
    12. the present study was the first to construct an adenovirus which coexpressed XAF1 and TNFalpha in the same open reading frame and expressed them proportionally. As AdXAF1&TNFalpha inhibited HCC cells with enhanced efficiency, it may be applicable for the treatment of HCC. PMID: 27121136
    13. SHC1 regulates the alternative splicing of XAF1 in extracellular matrix-detachment induced autophagy to coordinate with the anoikic cell death. PMID: 26643258
    14. Our study identified a novel splice variant of XAF1 in cancer cells PMID: 25216542
    15. Underexpression of XAF1 confers cisplatin-resistance in ovarian carcinoma. PMID: 25824780
    16. Restored XAF1 expression inhibits cell proliferation and induces cell apoptosis in human lung adenocarcinoma cell line A549. Furthermore, XAF1 may activate associated apoptotic signaling pathways in A549 cell line. PMID: 25539606
    17. Up-regulation of XAF1 distinctly increased apoptosis in colorectal cancer cells administered cisplatin. PMID: 25367849
    18. XAF1 stimulates HIPK2-mediated Ser-46 phosphorylation of p53 and XAF1 also steps up the termination of p53-mediated cell-cycle arrest by activating ZNF313. PMID: 25313037
    19. XAF1 as a possible sarcoidosis risk gene PMID: 24663488
    20. Transcriptionally active p73 induces translocation of XAF-1 during apoptosis caused by melphalan. XAF-1 directly interacts with Puma and promotes Bax translocation during melphalan-induced apoptosis. PMID: 24249729
    21. Promoter methylation of the XAF1 gene plays an important role in the carcinogenesis and progression of papillary thyroid carcinoma. PMID: 22944270
    22. Missense polymorphisms in XIAP-associated factor-1 is associated with papillary thyroid cancer. PMID: 23645777
    23. the role of XAF1 in promoting apoptosis in vascular endothelial cells after DENV2 infection. PMID: 23207547
    24. The search of XIAP binding region within XAF1 revealed a modest affinity XIAP(RING) binding site is located at the C-terminal portion of XAF1.We also mapped the interaction sites for XAF1(RBD) on XIAP(RING) by using NMR. PMID: 22811387
    25. XAF1 may be involved in ovarian cancer development and up-regulation of XAF1 may confer sensitivity of ovarian cancer cells to cisplatin-mediated apoptosis. PMID: 22759793
    26. XAF1 is frequently methylated in esophageal cancer, and XAF1 expression is regulated by promoter region hypermethylation. PMID: 22719195
    27. These findings suggest that a potential novel feedback loop exists between XAF1 and wild-type p53 PMID: 21678496
    28. The levels of XAF1 protein and mRNA in cancer tissues were significantly lower than those in cancer adjacent and normal lung tissues. PMID: 22088514
    29. 5-AZA treatment can induce the expression of XAF1 mRNA and protein in myeloma. PMID: 19549372
    30. 5-azacytidine treatment led to XAF1 promoter CpG islands hypomethylation and showed anti-myeloma activity in a time- and concentration-dependent manner. PMID: 19731821
    31. XAF1 down-regulation may contribute to the prostate cancer development PMID: 21143993
    32. Demonstrate that p53 could suppress the transcription of XAF1 through interaction with a high affinity responsive element (-95 to -86 nt) within XAF1 promoter in gastrointestinal cancer cells. PMID: 20198350
    33. INFalpha and 5-AZA-CdR can induce Xaf1 mRNA expressions in HL-60 and K562 cells. PMID: 18250043
    34. The high expression of HSF1 in gastro-intestinal cancer is associated with suppressed expression of XAF1. PMID: 17884799
    35. XAF1 is a valuable prognostic marker in pancreatic cancer and could be a potential candidate for cancer gene therapy. PMID: 19922503
    36. The expression levels of the pro-apoptotic XAF-1 gene modulate the cytotoxic response to Nutlin-3 in B chronic lymphocytic leukemia. PMID: 19847196
    37. XAF1 augments TRAIL-induced apoptosis PMID: 12029096
    38. Epigenetic silencing of XAF1 by aberrant promoter methylation may contribute to the malignant progression of human gastric tumors. PMID: 14612497
    39. XAF1 expression in melanoma tissues was significantly reduced compared with benign melanocytic nevi PMID: 15610524
    40. endogenous stress pressure in cancer cells sustained the high level expression of HSF1 and subsequently suppressed XAF1 expression, implicating the synergized effect of two anti-apoptotic protein families in cytoprotection under stress circumstances PMID: 16303760
    41. The ratio of XAF1(A) and XAF1C mRNA expression differs amongst the cell lines tested, suggesting differential mRNA stabilities and/or the existence of tissue- or cell type-specific splicing regulation. PMID: 16343440
    42. alternative splicing of XAF1 mRNA leads to formation of truncated XAF1 protein, which likely affects its functional interaction with XIAP, and consequently, contributes to pathogenesis of prostate cancers by disrupting balance of apoptosis machinery PMID: 16353137
    43. Gene expression incresed in multiple sclerosis patients treated with interferon-beta. PMID: 16459719
    44. siRNA to XAF1 inhibited IFN-induced apoptosis; conversely, overexpression of XAF1 overcame resistance to apoptosis induction by IFN-beta. PMID: 16801630
    45. Epigenetic alteration of XAF1 is frequent in human urogenital cancers and may contribute to the malignant progression of tumors by rendering tumor cells a survival advantage partially through the attenuated p53 response. PMID: 16909101
    46. Down-regulation of XAF1 in association with hypermethylation was detected in 3 of 4 human gastric cancer cell lines and 6 of 8 colon cancer cell lines. PMID: 17087954
    47. In cancerization of oral mucosa, XIAP protein could play an important antiapoptotic role by overexpression, while XAF1 protein does not appear to antagonize effectively the role of XIAP. PMID: 17331366
    48. Our study provides evidence that XAF1 is a crucial interferon-stimulated gene (ISG) mediator of IFN-induced sensitization to TRAIL in cancer. PMID: 17376236
    49. Low XAF1 mRNA expression levels relate to an unfavorable clinical course in renal cell carcinoma. PMID: 17449173
    50. An increased expression ratio of XIAP to XAF1 in combination with a disturbed expression of the XAF1 splice variants could be shown in gastric adenocarcinomas. PMID: 17471152

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Mitochondrion. Note=Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.; [Isoform 1]: Nucleus.; [Isoform 5]: Nucleus.
  • 组织特异性：
    Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
  • 数据库链接：

    HGNC: 30932

    OMIM: 606717

    KEGG: hsa:54739

    STRING: 9606.ENSP00000354822

    UniGene: Hs.441975