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  4. Recombinant Human ZW10 interactor (ZWINT)

Recombinant Human ZW10 interactor (ZWINT)

In Stock
  • 中文名称：
    人ZWINT重组蛋白
  • 货号：
    CSB-EP527261HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ZWINT
  • Uniprot No.：
  • 别名：
    Human ZW10 interacting protein 1; HZwint 1; HZwint1; KNTC 2 AP; KNTC2AP; MGC 117174; MGC117174; ZW10 interacting kinetochore protein; ZW10 interacting protein 1; ZW10 interactor; ZW10 interactor; kinetochore protein; ZW10-interacting protein 1; ZWINT 1; ZWINT; Zwint-1; ZWINT_HUMAN; ZWINT1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of BC020979
  • 来源：
    E.coli
  • 分子量：
    58.2kDa
  • 表达区域：
    1-277aa
  • 氨基酸序列
    MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
  • 基因功能参考文献：
    1. ZW10 interactor was frequently decreased in hepatocellular carcinoma. PMID: 30198401
    2. Our results provide the first evidence that Zwint-1 is required to correct erroneous kinetochore-microtubule attachment and regulate spindle checkpoint function during meiosis. PMID: 26486467
    3. the E3 ubiquitin ligase terf causes protein degradation of ZWINT and negatively regulates cell proliferation PMID: 22023800
    4. These studies identify zwint-1 as a novel AurB substrate required for kinetochore assembly and for proper spindle assembly checkpoint silencing at metaphase PMID: 21775627
    5. hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain PMID: 21345172
    6. show that ZW10 interacting protein-1(Zwint-1) is required and is sufficient for kinetochore localization of Zeste White 10 (ZW10) in HeLa cells PMID: 15485811
    7. Stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest. PMID: 18268100

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase.
  • 数据库链接：

    HGNC: 13195

    OMIM: 609177

    KEGG: hsa:11130

    STRING: 9606.ENSP00000363055

    UniGene: Hs.591363