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  4. Recombinant Human Zinc finger protein PLAG1 (PLAG1)

Recombinant Human Zinc finger protein PLAG1 (PLAG1)

  • 中文名称：
    人PLAG1重组蛋白
  • 货号：
    CSB-YP718773HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PLAG1重组蛋白
  • 货号：
    CSB-EP718773HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PLAG1重组蛋白
  • 货号：
    CSB-EP718773HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PLAG1重组蛋白
  • 货号：
    CSB-BP718773HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PLAG1重组蛋白
  • 货号：
    CSB-MP718773HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PLAG1
  • Uniprot No.：
  • 别名：
    COL1A2/PLAG1 fusion; FGFR1/PLAG1 fusion variant 3; Plag1; PLAG1_HUMAN; Pleiomorphic adenoma gene 1; Pleiomorphic adenoma gene 1 protein; PSA ; SGPA ; Zinc finger protein PLAG1; ZNF912
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-500
  • 氨基酸序列
    MATVIPGDLS EVRDTQKVPS GKRKRGETKP RKNFPCQLCD KAFNSVEKLK VHSYSHTGER PYKCIQQDCT KAFVSKYKLQ RHMATHSPEK THKCNYCEKM FHRKDHLKNH LHTHDPNKET FKCEECGKNY NTKLGFKRHL ALHAATSGDL TCKVCLQTFE STGVLLEHLK SHAGKSSGGV KEKKHQCEHC DRRFYTRKDV RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL LKVKTEPVDF LDPFTCNVSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS SGSAHQMITT LPLGMTCPID MDTVHPSHHL SFKYPFSSTS YAISIPEKEQ PLKGEIESYL MELQGGVPSS SQDSQASSSS KLGLDPQIGS LDDGAGDLSL SKSSISISDP LNTPALDFSQ LFNFIPLNGP PYNPLSVGSL GMSYSQEEAH SSVSQLPPQT QDLQDPANTI GLGSLHSLSA AFTSSLSTST TLPRFHQAFQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.
  • 基因功能参考文献：
    1. Rearrangement of PLAG1 and HMGA2 and expression of the corresponding proteins are frequent and specific findings in lacrimal gland pleomorphic adenoma (PA) and ca-ex-PA. PMID: 29437290
    2. PLAG1 is a microRNA target gene that is overexpressed in Wilms tumors with mutations in microRNA processing genes. PMID: 30026293
    3. HRAS mutations were more common in epithelial-myoepithelial carcinomas (EMCAs) with intact PLAG1 and HMGA2. Most EMCAs arose ex pleomorphic adenoma (PA)and the genetic profile of EMCA varies with the absence or presence of preexisting PA and its cytogenetic signature. Progression to higher grade EMCA with intact PLAG1 and HMGA2 correlates with the presence of TP53, FBXW7 mutations, or SMARCB1 deletion. PMID: 29135520
    4. Plag1 expression is lost in malignant transformation from pleomorphic adenoma to carcinoma ex pleomorphic adenoma. PMID: 27473265
    5. MiR-26a can facilitate occurrence of pituitary tumor and invasiveness, probably via inhibiting PLAG1 expression. PMID: 28012286
    6. expression of PLAG1 can be considered a valuable diagnostic marker for recurrent pleomorphic adenoma PMID: 27381214
    7. High PLAG1 expression is associated with pleomorphic adenoma. PMID: 26882287
    8. The sensitivity (55%) and specificity (75%) of PLAG1 in diagnosing PA in FNAs is relatively modest thus limiting its diagnostic utility. PMID: 27463119
    9. The Thr34 of PLAG1 is critical for LpMab-10 recognition. PMID: 26492619
    10. Data indicate that SLIT2, miR-218-1, RET/PLAG1 and SLIT2/ROBO1 pathway involved in Hirschsprung's disease. PMID: 25786906
    11. Altogether, these data suggest that HMGA2 is an upstream activator of PLAG1. PMID: 24516594
    12. Report illustrates two different tumorigenic pathways implicating PLAG1 in lipoblastoma: amplification through multiple copies of a small marker chromosome derived from chromosome 8, and a paracentric inversion of the long arm of chromosome 8. Review. PMID: 24433523
    13. The nuclear import of PLAG1 by KPNA2 is essential for the role of KPNA2 in HCC cells. PMID: 25060425
    14. a marker with good specificity for salivary gland pleomorphic adenomas PMID: 23958548
    15. Extraskeletal myxoid chondrosarcoma of the vulva with PLAG1 gene activation: molecular genetic characterization of 2 cases PMID: 24185117
    16. Fluorescence in situ hybridization for PLAG1 or HMGA2 can be used to distinguish between pleomorphic adenoma and carcinoma ex-pleomorphic adenoma and their morphologic mimics. PMID: 25439740
    17. lipoblastoma is a group of lipomatous tumors with PLAG1 rearrangement and overexpression. PMID: 24700772
    18. These results suggest that PLAG1 and CYLD do not play a role in ACC tumorigenesis. PMID: 23404581
    19. Lacrimal and salivary gland PAs and Ca-ex-PAs have similar genomic profiles and frequently overexpress the PLAG1 oncoprotein. Copy number gains involving 9p23-p22.3 (NFIB) and 22q12-qter (PDGFB) may be of importance for disease progression. PMID: 24468654
    20. Ten cases of salivary duct carcinoma had PLAG1 rearrangement/amplification (22.7%) and eight had HMGA2 (18.2%) rearrangement/amplification. PMID: 23738717
    21. PLAG1 not only activates genes that promote cell proliferation and tumor formation but also genes that inhibit these cellular processes. PMID: 23690029
    22. A subset of cutaneous and soft tissue ME tumors appears genetically linked to their salivary gland counterparts, displaying frequent PLAG1 gene rearrangements and occasionally LIFR-PLAG1 fusion. PMID: 23630011
    23. miR-141 contributes to fetal growth restriction by regulating PLAG1 expression. PMID: 23554918
    24. PLAG1 binding to the IGF2 P3 promoter and IGF2 expression is cell type-specific, and that the PLAG1 transcription factor acts as a transcriptional facilitator that partially overrides the insulation by the H19 ICR. PMID: 23023303
    25. We conclude that most Carcinoma ex pleomorphic adenoma, regardless of morphologic subtype,carry altered PLAG1 genes PMID: 22485045
    26. PLAG1 is evidently not involved in the development of myoepithelial tumours. The proportion of 8q12-alterations in myoepithelial tumours was very low. PMID: 22593475
    27. PLAG1 immunohistochemistry is useful for distinguishing lipoblastoma from other lipomatous tumors including liposarcoma. PMID: 22192798
    28. myoepithelial tumors with PLAG1 alteration share a common morphologic phenotype with salivary gland-like morphology and are genetically related to their salivary gland counterpart PMID: 22038920
    29. close relationship between cutaneous mixed tumors and pleomorphic adenomas of the salivary gland. However, the mechanism of PLAG1 expression in cutaneous mixed tumors appears to be possibly different from that of pleomorphic adenomas. PMID: 21927843
    30. present results also suggest that overexpression of PLAG1 is essential for the tumorigenesis of pleomorphic adenomas, although the mechanisms mediating PLAG1 overexpression seem to be variable. PMID: 21394649
    31. Overexpression of the oncogene PLAG1 is associated with the pathogenesis of chronic lymphocytic leukemia. PMID: 20687796
    32. Overexpression in transgenic mice causes hypereinsulinemic normoglycemia and insulin resistance. PMID: 20522588
    33. Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal PMID: 11882654
    34. overexpression of PLAG1 does not depend on chromosomal rearrangements in radiation associated pleomorphic adenomas PMID: 11894114
    35. PLAG1 regulates promoter P3-dependent transcription of IGF2 in hepatoblastomas. PMID: 14695992
    36. a direct repression of the transactivating capacity of the oncoprotein PLAG1 by SUMOylation PMID: 15208321
    37. PLAG1 amplification is associated with malignant mixed tumor of salivary gland PMID: 15262430
    38. fusion with HAS2 in lipoblastoma PMID: 15642402
    39. Finding reinforces the role of PLAG1 on the tumorigenesis of benign and malignant pleomorphic adenoma. PMID: 15920557
    40. Results establish the in vivo tumorigenic capacity of PLAG1 and indicate that the transgenic mice constitute a valuable model for pleomorphic salivary gland tumorigenesis and potentially for other glands as well. PMID: 15930271
    41. Overexpression of PLAG1 is associated with pleomorphic adenomas in transgenic mice PMID: 16108035
    42. PLAG1 protein is overexpressed in epithelial, myoepithelial, and mesenchymal-like tumor cells in tumors with fusions to CHCHD7 and TCEA1. PMID: 16736500
    43. The importance and versatility of the PLAG1 oncogene in tumourigenesis is discussed. PMID: 17332914
    44. PLAG1 gene rearrangements is associated with pleomorphic adenoma PMID: 17693184
    45. Molecular analyses in salivary gland tumors revealed that ring formation consistently generated novel FGFR1-PLAG1 gene fusions in which the 5'-part of FGFR1 is linked to the coding sequence of PLAG1 PMID: 18059337
    46. Fluorescence in situ hybridization (FISH) demonstrated rearrangements of the PLAG1 region in lipoblastoma in adolescencts and young adults. PMID: 18269579
    47. overexpression of PLAG1 is associated with pleomorphic adenomas of the salivary gland. PMID: 19347935
    48. a significant regulation of PLAG1 by miR-181a, miR-181b, miR-107, and miR-424. PMID: 19692702

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  • 相关疾病：
    A chromosomal aberration involving PLAG1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with constitutively expressed beta-catenin/CTNNB1. Fusion occurs in the 5'-regulatory regions, leading to promoter swapping between the 2 genes and activation of PLAG1 expression in adenomas. The chimeric transcript is formed by fusion of CTNNB1 exon 1 to PLAG1 exon 3. Reciprocal fusion transcript consisting of PLAG1 exon 1 and CTNNB1 exon 2-16 is also revealed in some adenomas (PubMed:9020842, PubMed:10029085). Translocation t(3;8)(p21;q12) with transcription elongation factor SII/TCEA1. The fusion transcript is composed of 5'-non-coding sequences as well as 63 nucleotides of the coding region of TCEA1 fused to the acceptor splice site of PLAG1 exon 3. The fusion transcript encodes a truncated TCEA1-PLAG1 protein of 90 AA as well as an apparently normal PLAG1 protein. Reciprocal fusion transcript PLAG1-TCEA1 is also present in one adenoma (PubMed:10029085, PubMed:16736500). Translocation t(5;8)(p13;q12) with leukemia inhibitory factor receptor LIFR. This fusion occured in the 5'-non-coding sequences of both genes, exchanging regulatory control element while preserving the coding sequences (PubMed:9525740). Translocation t(6;8)(p21.3-22;q13) with Coiled-coil-helix-coiled-coil-helix domain-containing protein 7/CHCHD7. Fusion occurs in the 5' regulatory regions, leading to promoter swapping and up-regulation of PLAG1 expression (PubMed:16736500). Ectopic expression of PLAG1 under the control of promoters of distinct translocation partner genes is a general pathogenetic mechanism for pleiomorphic adenomas with 8q aberrations. These fusion genes are likely to be found in adenomas with normal karyotype as this subgroup of tumors also exhibit PLAG1 activation (PubMed:9020842, PubMed:10029085, PubMed:9525740, PubMed:16736500).
  • 亚细胞定位：
    Nucleus. Note=Strong nucleolar localization when sumoylation is inhibited.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 组织特异性：
    Expressed in fetal tissues such as lung, liver and kidney. Not detected or weak detection in normal adult tissues, but highly expressed in salivary gland with benign or malignant pleiomorphic adenomas with or without 8q12 aberrations, with preferential oc
  • 数据库链接：

    HGNC: 9045

    OMIM: 181030

    KEGG: hsa:5324

    STRING: 9606.ENSP00000325546

    UniGene: Hs.14968