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  4. Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag)

Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag)

  • 货号：
    CSB-YP361309HKP
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP361309HKP
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP361309HKP-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP361309HKP
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP361309HKP
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    gag; Gag polyprotein; Pr55Gag) [Cleaved into: Matrix protein p17; MA); Capsid protein p24; CA); Spacer peptide 1; SP1; p2); Nucleocapsid protein p7; NC); Spacer peptide 2; SP2; p1); p6-gag]
  • 种属：
    Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)
  • 蛋白长度：
    partial of Capsid protein p24 chain
  • 表达区域：
    133-338
  • 氨基酸序列
    PIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi).; Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus. Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA.; Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry. Host restriction factors such as monkey TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating. On the other hand, interactions with PDZD8 or CYPA stabilize the capsid.; Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly.; Plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.
  • 基因功能参考文献：
    1. these results demonstrate a novel role of the p6 domain in the specificity of Pr55(Gag)-RNA interactions, and strongly suggest that the p6 domain contributes to the discrimination of HIV-1 gRNA from cellular and spliced viral mRNAs, which is necessary for its selective encapsidation. PMID: 29954247
    2. High-resolution structures of HIV-1 Gag cleavage mutants determine structural switch for virus maturation. PMID: 30217893
    3. Amyloid precursor protein (APP) binds the HIV-1 Gag polyprotein, retains it in lipid rafts and blocks HIV-1 virion production and spread. PMID: 29142315
    4. The capsid protein protects HIV-1 complexes from degradation, mediates docking at the nuclear pore before uncoating, and determines the depth of nuclear penetration en route to integration. PMID: 29649444
    5. observations indicate that PACSIN2 promotes the cell-to-cell spreading of HIV-1 by connecting Gag to the actin cytoskeleton. PMID: 29891700
    6. Increasing CpG dinucleotide contents in gag gene decreases the stability and abundance of intracellular HIV-1 genomic RNA, also reduces Gag expression and virion production. PMID: 29121951
    7. Staufen1 is capable of inhibiting NC-induced stress granule assembly PMID: 29127210
    8. The s show for the first time that HIV-1 Gag self-assembly is responsible for the formation of phosphatidylinositol 4,5-diphosphate lipid nanoclusters, enriched in cholesterol but not in sphingomyelin. PMID: 28008947
    9. Data report that p17 is released in an active form by Gag-expressing cells. The secretion of p17 takes place at the plasma membrane and involves the interaction of p17 with PI(4,5)P2. The release of p17 occurs following its cleavage from Pr55Gag by cellular aspartyl proteases. PMID: 27905556
    10. The s propose that HIV-1 genomic RNA is selectively packaged because binding to a cis-acting RNA element called the 'packaging signal' (Psi) nucleates virion assembly with particular efficiency. PMID: 28726630
    11. Data show that Gag-protease encounter complexes are primarily mediated by interactions between protease and the globular domains of Gag. PMID: 27791180
    12. These results reveal an important role of phosphatidylinositol 4,5-diphosphate for HIV-1 morphogenesis beyond Gag recruitment to the plasma membrane and suggest a dynamic equilibrium of Gag-lipid interactions. PMID: 28574338
    13. The s found that the overexpression of YTHDF proteins in cells inhibited HIV-1 infection mainly by decreasing HIV-1 reverse transcription, while knockdown of YTHDF1-3 in cells had the opposite effects. Moreover, silencing the N(6)-methyladenosine writers decreased HIV-1 Gag protein expression in virus-producing cells, while silencing the N(6)-methyladenosine erasers increased Gag expression. PMID: 27371828
    14. Two ribosome recruitment sites direct multiple translation events within HIV1 Gag open reading frame. PMID: 28449096
    15. Results indicate that Vpr overcomes the effects of TSG101 overexpression to support viral production by competing with TSG101 to bind Gag. PMID: 27648839
    16. In fact, induction of autophagy in lymphatic endothelial cells by HIV-1 p17 promotes lymphangiogenesis, whereas pharmacological and genetic inhibition of autophagy inhibits p17-triggered lymphangiogenesis. PMID: 28592537
    17. Specific dimeric viral RNA-Gag interactions are the nucleation event of infectious virion assembly, ensuring that one RNA dimer is packaged into each nascent HIV-1 virion. PMID: 28539452
    18. The s identified Gag residues 483 and 484, located within the Alix-binding motif involved in virus budding, as major contributors to subtype-specific replicative differences. PMID: 28424286
    19. It was shown that in subtype C, the duplication of the PTAP motif in p6 Gag involves sequence stretches of greater length, and at a much higher frequency as compared to other HIV-1 subtypes. PMID: 28118816
    20. Enhanced Immune Responses against HIV-1 with Adenovector (Gag and Tat) Prime/Protein Boost Regimen and GM-CSF Injection. PMID: 27917627
    21. Gag NC domain interacts with the ribosomal protein RPL7 endowed with nucleic acid chaperone activity, favoring the notion that RPL7 could be a Gag helper chaperoning factor possibly contributing to the start of Gag assembly PMID: 27515235
    22. Taken together, these data demonstrate that cytotoxic-T-lymphocyte-driven escape mutations within p24 Gag restricted by protective HLA class I alleles have a significant impact on HIV-1 capsid stability that might contribute to the persistent control of viral replication observed despite viral escape from cytotoxic-T-lymphocyte responses. PMID: 27279617
    23. Altogether, these data revealed that targeting of HIV-1 Gag to the virus-containing compartments in macrophages requires the NC domain-dependent multimerization. PMID: 27440886
    24. Mutational heterogeneity has been found in p6 Gag late assembly (l) domains in HIV-1 subtype C viruses from South Africa. PMID: 26413747
    25. Gag is able to block the assembly of type II noncanonical stress granules to facilitate continued Gag protein synthesis. PMID: 27025252
    26. The s find that HIV-1 nucleocapsid mimics the PDZ domains of syntenin, a membrane-binding adaptor involved in cell-to-cell contact/communication, to capture the Bro1 domain of ALIX, which is an ESCRTs recruiting cellular adaptor. PMID: 26962944
    27. These data demonstrate that mutations in the CypA binding site of the capsid resulted in higher capsid stability and hampered infectivity in macrophages. PMID: 26414211
    28. These results indicate that HIV-1 capsid is involved in post-nuclear entry steps preceding integration. PMID: 27107820
    29. Data indicate that distinct Gag protein binding sites were identified on exposed RNA surfaces corresponding to regions. PMID: 26449409
    30. HIV-1 nucleocapsid protein localizes efficiently to the nucleus and nucleolus. PMID: 26967976
    31. HIV-1 depends on host-cell-encoded factors to complete its life cycle; data suggest NAF1 (HIV Nef-associated factor 1) promotes nuclear export of un-spliced HIV-1 gag mRNA; association between NAF1 and CRM1 (exportin 1) is required for this function. PMID: 26733199
    32. p2 peptide from HIV-1 enhances HIV-1 acute infection by increasing intracellular ATP production via the activation of mitochondrial cytochrome c oxidase (MT-CO) involved in the respiratory chain PMID: 26577226
    33. Data suggest that minus-strand strong-stop DNA exhibits four binding sites for NC (nucleocapsid protein, 55-amino acid gene product of gag); NC-mediated annealing process does not rely on a single pathway (zipper intermediate or kissing complex). PMID: 26668324
    34. Dynamic interactions occur among HIV-1 RNAs, and stabilization of the RNA dimer requires Gag protein. PMID: 26712001
    35. The data suggest that once the SP1 Region of HIV-1 Gag becomes helical, its propensity to self-associate could contribute to Gag-Gag interactions and thus to particle assembly. PMID: 26637452
    36. These data support a model in which the major homology region residues act just after membrane targeting, with some residues promoting stability and another promoting multimerization of the membrane-targeted assembling Gag oligomer. PMID: 26656702
    37. host factor HLA plays a stronger role in disease progression than the Nef and Gag sequence variations in HIV-1-infected Kenyan children PMID: 26317223
    38. The s observed that the Pro-Thr-Ala-Pro duplication in p6(Gag) significantly increased the infectivity and replication capacity of the virus compared to those of viruses bearing only resistance mutations in protease. PMID: 26512081
    39. The s report that HIV-1 exploits the host factor RuvB-like 2 (RVB2) to balance relative expression of Gag and Env for efficient production of infectious virions. PMID: 26211835
    40. Analyzed mutation patterns in Gag and protease of protease inhibitor resistant HIV-1 clinical isolates. PMID: 25894830
    41. Nedd4 co-injection was found to have no affect on Gag- or Env-specific IFNgamma but had a trend of increased Gag-specific IL-6, IL-17A and TNFalpha that was not seen following Env stimulation. PMID: 24614057
    42. Data show that a subset of preferential nucleocapsid (NC) protein binding sites is responsible for the observed changes in the TAR RNA unfolding landscape. PMID: 26483503
    43. Overexpression of either Tra2alpha or Tra2beta results in a marked reduction in HIV-1 Gag/ Env expression. PMID: 25970345
    44. The high prevalence of MxB-resistant mutations in the CypA-binding loop indicates the significant selective pressure of MxB on HIV-1 replication in vivo. PMID: 25571928
    45. Data show that capsid protein p24-DsRed-Monomer was co-localized with tripartite motif containing 22 (TRIM22)-EGFP in HEK293T cells. PMID: 26271984
    46. Nonmyristylated HIV Gag retains the preference for only phosphatidylinositol-(4,5)-bisphosphate containing an unsaturated acyl chain. PMID: 25995263
    47. the non-myristoylated Pr55(gag) expressed in tobacco plastids was likely able, through the HBR motif, to bind to nonphosphorous glycerogalactolipids or other classes of lipids present in plastidial membranes. PMID: 25348481
    48. primary Pr55(Gag) binding site on the gRNA consists of the internal loop and the lower part of stem-loop 1, the upper part of which initiates gRNA dimerization PMID: 24986025
    49. An HIV-1 variant carrying a conservative S40N exchange in p6(Gag) is fully functional in tissue culture demonstrating that neither S40 nor its phosphorylation are required for HIV-1 release and maturation. PMID: 25524645
    50. Data show that plasmacytoid dendritic cell and HIV Gag p55-specific CD8+ perforin+ IFN-gamma+ T cells can inform set-point plasma HIV VL after antiretroviral treatment. PMID: 25684333

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  • 亚细胞定位：
    [Gag polyprotein]: Host cell membrane; Lipid-anchor. Host endosome, host multivesicular body.; [Matrix protein p17]: Virion membrane; Lipid-anchor. Host nucleus. Host cytoplasm.; [Capsid protein p24]: Virion.; [Nucleocapsid protein p7]: Virion.
  • 蛋白家族：
    Primate lentivirus group gag polyprotein family
  • 数据库链接：

    KEGG: vg:155030