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  4. Recombinant Human papillomavirus type 16 protein E4 (E4)

Recombinant Human papillomavirus type 16 protein E4 (E4)

  • 货号：
    CSB-YP356968HML
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356968HML-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356968HML
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356968HML
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    E4
  • Uniprot No.：
  • 别名：
    E4; Protein E4; E1^E4
  • 种属：
    Human papillomavirus type 16
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-95
  • 氨基酸序列
    YYVLHLCLAA TKYPLLKLLG STWPTTPPRP IPKPSPWAPK KHRRLSSDQD QSQTPETPAT PLSCCTETQW TVLQSSLHLT AHTKDGLTVI VTLHP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Contributes to multiple aspects of the viral life cycle including viral genome amplification, suppression of suprabasal cell differentiation and egress of newly formed virions. Induces host cell cycle arrest at the G2 phase by associating with and preventing the nuclear entry of host CDK1/cyclin B1 complexes. Inhibits cellular DNA replication by preventing loading of host replication licensing proteins MCM2 and MCM7 onto chromatin. Within the cytoplasm, associates with host kinase SRPK1, a splicing factor regulator, and inhibits its activity. Therefore, E4 favors expression of late viral transcripts by inhibiting SRPK1-mediated phosphorylation of host serine-arginine (SR) proteins that have critical roles in mRNA metabolism. Late in the infectious cycle, E4 also acts to diminish the integrity of the keratinocyte by disrupting the keratin cytoskeleton and inducing apoptosis through alteration of mitochondrial function to facilitate egress of the newly formed virions.
  • 基因功能参考文献：
    1. These results suggest that 16E4-mediated enhancement of genome amplification involves its cell cycle inhibition and cellular kinase activation functions, with E4 modifying the activity and function of viral replication proteins including E1 PMID: 28306742
    2. Expression of the HPV16 E4 and E5 proteins results in a substantial change in the composition of the cell membrane and extracellular space, associated with alterations in cell adhesion and differentiation. PMID: 24898764
    3. 20 cervical cyology samples with HPV16 were tested by RT-PCR for HPV16 E4 mRNA, found in sqaumous intraepithelial lesion and squamous carcinoma, but not in samples negative for intrepithelial lesion. E4 expression assay may be useful in cancer diagnosis. PMID: 22299437
    4. The data suggest a model in which the expression of 16E5 from the major E1--E4-E5 mRNA promotes T57 phosphorylation of E1--E4 by ERK and keratin binding. PMID: 19211765
    5. identified the N-terminus of E2 as the first example of a viral protein that directly binds the E1--E4 protein PMID: 19783272

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  • 亚细胞定位：
    Host cytoplasm. Host nucleus.
  • 蛋白家族：
    Papillomaviridae E4 protein family
  • 数据库链接：

    KEGG: vg:1489076