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  4. Recombinant Human tRNA methyltransferase 10 homolog C (TRMT10C)

Recombinant Human tRNA methyltransferase 10 homolog C (TRMT10C)

  • 中文名称：
    人TRMT10C重组蛋白
  • 货号：
    CSB-YP748718HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TRMT10C重组蛋白
  • 货号：
    CSB-EP748718HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TRMT10C重组蛋白
  • 货号：
    CSB-EP748718HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TRMT10C
  • Uniprot No.：
  • 别名：
    TRMT10C; MRPP1; RG9MTD1tRNA methyltransferase 10 homolog C; HBV pre-S2 trans-regulated protein 2; Mitochondrial ribonuclease P protein 1; Mitochondrial RNase P protein 1; RNA; guanine-9-)-methyltransferase domain-containing protein 1; Renal carcinoma antigen NY-REN-49; mRNA methyladenosine-N(1)-methyltransferase; EC 2.1.1.-; tRNA; adenine(9)-N(1))-methyltransferase; EC 2.1.1.218; tRNA; guanine(9)-N(1))-methyltransferase; EC 2.1.1.221
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    40-403
  • 氨基酸序列
    M SSKIPAVTYP KNESTPPSEE LELDKWKTTM KSSVQEECVS TISSSKDEDP LAATREFIEM WRLLGREVPE HITEEELKTL MECVSNTAKK KYLKYLYTKE KVKKARQIKK EMKAAAREEA KNIKLLETTE EDKQKNFLFL RLWDRNMDIA MGWKGAQAMQ FGQPLVFDMA YENYMKRKEL QNTVSQLLES EGWNRRNVDP FHIYFCNLKI DGALHRELVK RYQEKWDKLL LTSTEKSHVD LFPKDSIIYL TADSPNVMTT FRHDKVYVIG SFVDKSMQPG TSLAKAKRLN LATECLPLDK YLQWEIGNKN LTLDQMIRIL LCLKNNGNWQ EALQFVPKRK HTGFLEISQH SQEFINRLKK AKT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mitochondrial tRNA N(1)-methyltransferase involved in mitochondrial tRNA maturation. Component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends. Together with HSD17B10/MRPP2, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity. The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)-methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs; TRMT10C/MRPP1 acting as the catalytic N(1)-methyltransferase subunit. The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme. In addition to tRNA N(1)-methyltransferase activity, TRMT10C/MRPP1 also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA. Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.
  • 基因功能参考文献：
    1. in addition to being an essential component of the RNase P reaction, MRPP1/2 serves as a processing platform for several down-stream tRNA maturation steps in human mitochondria. PMID: 29040705
    2. identifying mutations in TRMT10C as a cause of mitochondrial disease and highlighting the importance of RNA processing for correct mitochondrial function PMID: 27132592
    3. Inhibition of mitochondrial RNase P by beta-amyloid is an unspecific effect and is not mediated by beta-amyloid interaction with SDR5C1. PMID: 23755257
    4. MRPP1 is essential for transcript processing, RNA modification, translation and mitochondrial respiration. PMID: 21857155
    5. We also identify the 5' and 3' transcript ends of the three lncRNAs and show that mitochondrial RNase P protein 1 (MRPP1) is important for the processing of these transcripts. PMID: 22028365

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  • 相关疾病：
    Combined oxidative phosphorylation deficiency 30 (COXPD30)
  • 亚细胞定位：
    Mitochondrion matrix, mitochondrion nucleoid.
  • 蛋白家族：
    Class IV-like SAM-binding methyltransferase superfamily, TRM10 family
  • 数据库链接：

    HGNC: 26022

    OMIM: 615423

    KEGG: hsa:54931

    STRING: 9606.ENSP00000312356

    UniGene: Hs.594839