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  4. Recombinant Human xcitatory amino acid transporter 3 (SLC1A1), partial

Recombinant Human xcitatory amino acid transporter 3 (SLC1A1), partial

  • 中文名称：
    人SLC1A1重组蛋白
  • 货号：
    CSB-EP021432HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    EAA3_HUMAN; EAAC 1; EAAC1; EAAT 3; Excitatory amino acid carrier 1 ; Excitatory amino acid carrier1; Excitatory amino acid transporter 3; Excitatory amino acid transporter3; Excitatory amino-acid carrier 1; GLUTAMATE TRANSPORTER; HIGH-AFFINITY; MEAAC 1; MEAAC1; Neuronal and epithelial glutamate transporter; REAAC 1; REAAC1; Slc1 a1; Slc1a 1; SLC1A1; Sodium dependent glutamate/aspartate transporter 3; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter; system Xag); member 1; SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER); MEMBER 1; Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter; system Xag); member 1; Solute carrier family 1 member 1; Solute carrier family 1; member 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    37.5kDa
  • 表达区域：
    430-524aa
  • 氨基酸序列
    AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport. Negatively regulated by ARL6IP5.
  • 基因功能参考文献：
    1. mir-26a and SLC1A1 may play a role in the Multiple Sclerosis pathogenesis, and may be potential targets for the development of new biomarkers and/or therapeutic tools. PMID: 28962897
    2. SLC1A1 and DPF3 were strongly associated with idiopathic male infertility and were significantly correlated with semen quality alterations PMID: 27232852
    3. results suggest that six common SNPs of SLC1A1 may not contribute to the development of obsessive compulsive disorder, but may contribute to certain aspect of trait empathy such as personal distress PMID: 29304071
    4. observations of EAAT3 regulation in patients is giving us insight into the endogenous function of this transporter, as well as the consequences of altered function--{REVIEW} PMID: 27233497
    5. Our study showed a lack of association between SLC1A1 variants and normal tension glaucoma in Japanese patients, suggesting that the SLC1A1 gene does not play a critical role in the development of the disorder in this patient population. PMID: 26771863
    6. Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif. PMID: 27358480
    7. observations provide insights into the molecular basis of hot water epilepsy and show the role of SLC1A1 variants in this intriguing neurobehavioral disorder PMID: 28324217
    8. This study demonstrated that Variations within SLC1A1 are associated with risk of epileptogenesis following posttraumatic brain injury. PMID: 27153812
    9. Lithium-sensitive GSK3ss is a powerful regulator of excitatory amino acid transporters EAAT3 and EAAT4. PMID: 27978527
    10. Data suggest that EAAC1 transporters present on NST dendrites may play a minor role if any in glutamate clearance. PMID: 25515313
    11. SPAK and OSR1 are negative regulators of EAAT3 activity PMID: 26112741
    12. Excitatory amino acid carrier 1 (EAAC1) plays a pivotal role in neuronal glutathione synthesis to maintain cellular redox homeostasis. PMID: 26007177
    13. Transport of either L-glutamate or L-selenocysteine by EAAT3 decreased intracellular pH, whereas transport of cysteine resulted in cytoplasmic alkalinization. PMID: 25275463
    14. Rs301430 is a T/C functional polymorphism that influences ago of onset in obsessive-compulsive disorder. PMID: 24768158
    15. variability within the SLC1A1 gene impacts both the presence and severity of posttraumatic stress disorder among a sample of combat-exposed veterans. PMID: 25445080
    16. Our data suggest that SLC1A1 is unlikely to be a major susceptibility gene for schizophrenia in Han Chinese PMID: 23931931
    17. Results indentify plausible candidates include non-recurrent deletions at the glutamate transporter gene SLC1A1, a CNV locus recently suggested to be involved in schizophrenia through linkage analysis, and duplications at 1p36.33 and CGNL1. PMID: 24163246
    18. internalization of EAAT3 triggered by amphetamine increases glutamatergic signaling and thus contributes to the effects of amphetamine on neurotransmission. PMID: 25033183
    19. EAAT3/EAAC1 expression is altered in pathological conditions, such as hypoxia/ischemia, multiple sclerosis, schizophrenia, and epilepsy. (Review) PMID: 24162932
    20. SLC1A1, GRIN2B, and interactions between the two may potentially confer a susceptibility to obsessive-compulsive symptoms in schizophrenia patients receiving clozapine. PMID: 23660601
    21. This study demonistrated that SLC!A1 no association with obsessive-compulsive disorder in Han Chinese population. PMID: 23411042
    22. A single-marker association study of several single-nucleotide polymorphisms in the SLC1A1 genomic region was performed. PMID: 22776887
    23. Individuals with schizophrenia carrying at least one C allele of rs35753505 (SNP8NRG221533) show decreased expression of SLC1A6 in the molecular layer of both cerebellar hemispheres, compared to individuals homozygous for the T allele. PMID: 22424243
    24. This study demonisrrated that glutamate aspartate transporter (GLAST) within the human cochlea in patient with Meniere's disease. PMID: 23850643
    25. P259R alters EAAT3 transport functions by decelerating conformational changes associated with sodium binding. PMID: 24214974
    26. isoforms negatively modulate glutamate uptake and are differentially expressed in subjects with obsessive-compulsive disorder PMID: 23695234
    27. The study provided suggestive evidence that the SLC1A1 rs10491734 was significantly associated with early-onset OCD in the Han Chinese population. PMID: 23564280
    28. revealed weak association between OCD and one of nine tested SLC1A1 polymorphisms PMID: 23606572
    29. A deletion at the SLC1A1 glutamate transporter gene originally identified as a copy number variant exclusively carried by members of a 5-generation Palauan family, was investigated. PMID: 23341099
    30. The function of the one SLC1A1 missense variant reported previously in obsessive-compulsive disorder, Thr164Ala, was characterized and found that the Ala164 allele leads to decreased Vmax and Km. PMID: 22617815
    31. Three single nucleotide polymorphisms in SLC1A1 (rs2228622, rs3780412 and rs3780413), which had been associated with second-generation antipsychotic agents-induced obsessive-compulsive symptoms, were investigated. PMID: 22531293
    32. rapid extracellular K(+) application to EAAC1 under single turnover conditions (K(+) inside) resulted in outward transient current. We propose a charge compensation mechanism, in which the C-terminal transport domain bears overall negative charge of -1.23 PMID: 22707712
    33. interaction of NCX1 and EAAC1 transporters leads to glutamate-enhanced ATP production in brain mitochondria PMID: 22479505
    34. Neutralizing aspartate 83 modifies substrate translocation of excitatory amino acid transporter 3 (EAAT3) glutamate transporters. PMID: 22532568
    35. mTOR is a novel powerful regulator of EAAT3 and may thus contribute to protection against neuroexcitotoxicity. PMID: 22483750
    36. These findings suggest the involvement of SLC1A1 in the pathogenesis of schizophrenia. PMID: 22095641
    37. This study provides suggestive evidence that DLGAP3 and its interactive effect with SLC1A1 might be involved in susceptibility to developing OC symptoms in schizophrenia patients receiving AAP treatment. PMID: 21990008
    38. genotyped an additional 111 SNPs in or near SLC1A1, covering from 9 kb upstream to 84 kb downstream of the gene at average spacing of 1.7 kb per SNP, and conducted family-based association analyses in 1,576 participants in 377 families PMID: 21445956
    39. SLC1A1 mutations leading to substitution of arginine to tryptophan at position 445 (R445W) and deletion of isoleucine at position 395 (I395del) cause dicarboxylic aminoaciduria, an autosomal recessive disorder of urinary glutamate and aspartate transport PMID: 21123949
    40. SLC1A1 alleles may have a role in obsessive-compulsive disorder PMID: 19569082
    41. Cysteine uptake by EAAC1 is important for zinc homeostasis and neuronal antioxidant function following transient cerebral ischemia. PMID: 21084597
    42. association of SNP rs301430 with severity of repetitive behaviors and anxiety in autism spectrum disorder PMID: 20155310
    43. suggests that T370S also alters the cation selectivity of this cation-binding site, as expected if T370 forms part of a cation-binding site in EAAT3. PMID: 20634426
    44. the side chain of EAAC1 Met-367 fulfills a steric role in the positioning of the substrate in the binding pocket in a step subsequent to its initial binding PMID: 20424168
    45. analysis of cation binding to the glutamate transporter EAAC1 probed with mutation of the conserved amino acid residue Thr10 PMID: 20378543
    46. PIP5K2A is a novel signaling element in the regulation of EAAT3 activity PMID: 19644675
    47. Dynamic equilibrium between coupled and uncoupled modes of a neuronal glutamate transporter PMID: 11823462
    48. data show specific localization of glutamate transporters EAAT1, EAAT2 and EAAT3 in the human placenta during development PMID: 15135231
    49. EAAT3 has a big extracellular vestibule, but does not undergo a large-scale motion to another state. Short channels connect the intracellular solution to the vestibule. Small conformational changes & glutamate uptake, occur independently in each subunit. PMID: 15716409
    50. enhanced expression of glutamate transporters in multiple sclerosis constitutes a regulatory response of glial cells to toxic levels of glutamate in the CNS during inflammation and neurodegeneration PMID: 16061389

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  • 相关疾病：
    Dicarboxylic aminoaciduria (DCBXA); Schizophrenia 18 (SCZD18)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. Early endosome membrane. Late endosome membrane. Recycling endosome membrane.
  • 蛋白家族：
    Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A1 subfamily
  • 组织特异性：
    Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
  • 数据库链接：

    HGNC: 10939

    OMIM: 133550

    KEGG: hsa:6505

    STRING: 9606.ENSP00000262352

    UniGene: Hs.444915