Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Influenza A virus Nuclear export protein (NS)

Recombinant Influenza A virus Nuclear export protein (NS)

  • 中文名称：
    Recombinant Influenza A virus Nuclear export protein(NS),Yeast
  • 货号：
    CSB-YP874060IEF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Influenza A virus Nuclear export protein(NS),Yeast
  • 货号：
    CSB-EP874060IEF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Influenza A virus Nuclear export protein(NS),Yeast
  • 货号：
    CSB-EP874060IEF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Influenza A virus Nuclear export protein(NS),Yeast
  • 货号：
    CSB-BP874060IEF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Influenza A virus Nuclear export protein(NS),Yeast
  • 货号：
    CSB-MP874060IEF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NS
  • Uniprot No.：
  • 别名：
    NS; Nuclear export protein; NEP; Non-structural protein 2; NS2
  • 种属：
    Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-121
  • 氨基酸序列
    MDSNTITSFQ DILQRMSKMQ LESSSVDLNG MITQFERLKI YRDSLGESMM RMGDLHSLQN RNATWRNELS QKFEEIRWLI AECRNILTKT ENSFEQITFL QALQLLLEVE SEIRTFSFQL I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs bound to the protein M1. Since protein M1 is not available in large quantities before late stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the host nucleus until sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enter the host cytoplasm only when associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.
  • 基因功能参考文献：
    1. Ubiquitin and SUMO compete for the same lysine (K242) on influenza A virus M1 and the interaction of viral NS2 with human AIMP2 facilitates the switch of the M1 modification from ubiquitination to SUMOylation, thus increasing viral replication. PMID: 25320310
    2. Although NEP stimulates viral RNA synthesis by binding to the viral polymerase, its function during nuclear export implicates interaction with viral ribonucleoprotein (vRNP)-associated M1. PMID: 24891509
    3. Adaptive mutations in NEP compensate for defective H5N1 RNA replication in mammalian cells. PMID: 22549831
    4. The high pathogenic strains of the avian influenza H5N1 virus isolated in Kazakhstan have NS of different genotypes. A/swan/Mangystau/3/2006 strain is of NS2A genotype that is typical for Gs/Gd-like strains. PMID: 21338659
    5. Deletion of nucleotides of NS gene from 263 to 277 sites increases H5N1 pathogenesis in chicken. PMID: 16736596
    6. results indicate that the NS1 gene is critical for the pathogenicity of avian influenza virus in chickens and that the amino acid residue Ala149 correlates with the ability of these viruses to antagonize interferon induction in chick embryo fibroblasts PMID: 16971424
    7. Amino acid from 80 to 84 involved in virus resistance against cytokinewas was deleted in NS1. PMID: 17672298
    8. 15-nucleotide deletion of NS gene from site 263 to 277 increased the virulence of H5N1 viruses in chickens and mice. PMID: 18317917
    9. NS1 protein of H5N1 down-regulates the expression level of IP-10. PMID: 19031697
    10. During the process of serial passages in ducks, only the NS gene developed non-synonymous substitutions, which caused two mutations in NS1 protein (Val23Ala and Leu207Pro) and one in NS2 (Phe55Leu). PMID: 19802744

    显示更多

    收起更多

  • 亚细胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Influenza viruses NEP family
  • 数据库链接：

    KEGG: vg:3654622