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Recombinant Klebsiella pneumoniae Metallo-beta-lactamase type 2 (blaNDM-1)

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  • 中文名称：
    Recombinant Klebsiella pneumoniae Metallo-beta-lactamase type 2(blaNDM-1)
  • 货号：
    CSB-EP510513KBG
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP510513KBG could indicate that this peptide derived from E.coli-expressed Klebsiella pneumoniae blaNDM-1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP510513KBG could indicate that this peptide derived from E.coli-expressed Klebsiella pneumoniae blaNDM-1.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    blaNDM-1
  • Uniprot No.：
  • 别名：
    blaNDM-1Metallo-beta-lactamase type 2; EC 3.5.2.6; B2 metallo-beta-lactamase; Beta-lactamase type II; Metallo-beta-lactamase NDM-1; Metallo-beta-lactamase type II; New Delhi metallo-beta-lactamase-1; NDM-1
  • 种属：
    Klebsiella pneumoniae
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    41.6kDa
  • 表达区域：
    29-270aa
  • 氨基酸序列
    GEIRPTIGQQMETGDQRFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHSLTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am looking for NDM-1 protein from E.coli/ Kpn source for measurement of IC50's of small molecule inhibitors on the catalytic activity of the enzyme, using a chromogenic substrate like Nitrocefin/CENTA or Penicillin G.
Please let me know if Recombinant bacterial metallo beta lactamase NDM-1 can be used for catalytic activity measurements. I am confused about the exact expression region of the protein (164-222aa) and the size of the protein (~13-14KDa? or it is ~7KDa?). Please clarify.

A:
Very nice to receive your inquiry. Kpn should refer to Klebsiella pneumoniae, the Cat No you mentioned corresponds to the region of 29-270aa, but later you mentioned the region of 164-222aa.
We can provide both of the two regions, please check the quotation as follows:
Recombinant Klebsiella pneumoniae Metallo-beta-lactamase type 2(blaNDM-1)
CSB-EP510513KBG >> E.coli N-terminal 6xHis-SUMO-tagged
CSB-EP510513KBGa0 >> E.coli N-terminal 6xHis-tagged
Expression Region: 29-270aa; Full length of the mature protein.
Sequence:

GEIRPTIGQQMETGDQRFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHSLTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR


Recombinant Klebsiella pneumoniae Metallo-beta-lactamase type 2(blaNDM-1),partial
CSB-YP510513KBG1 >> Yeast
CSB-EP510513KBG1 >> E.coli
CSB-BP510513KBG1 >> Baculovirus
CSB-MP510513KBG1 >> Mammalian cell
Expression Region: 164-222aa; Partial.
Tag information: Tag type will be determined during the manufacturing process.
Sequence:

AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG


CSB-EP510513KBG1 Expression Region: 164-222aa; The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged
The Mw of the target protein is around 6kDa, and the Mw of the tag is around 5kDa, so the final Mw of the fusion protein is about 11kDa.
Q:

I am in need of full length NDM-1 from E.coli/Kpn expressed in E.coli, that is enzymatically active.
What is the difference between CSB-EP510513KBG >> E.coli and CSB-EP510513KBGa0 >> E.coli ?
Can you provide references where these proteins are used for in vitro catalytic activity (IC50) measurements (hydrolysis of carbapenems).

A:
Thanks for your inquiry. The difference between CSB-EP510513KBG >> E.coli and CSB-EP510513KBGa0 >> E.coli is the tag, CSB-EP510513KBG: N-terminal 6xHis-SUMO-tagged; CSB-EP510513KBGa0: N-terminal 6xHis-tagged;
We can provide these proteins, but as we haven't tested the activity, so we can't guarantee it's enzymatically active.
Sorry, there isn't any references where these proteins are used for in vitro catalytic activity (IC50) measurements (hydrolysis of carbapenems).

靶点详情

  • 功能：
    Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin.
  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    Metallo-beta-lactamase superfamily, Class-B beta-lactamase family
  • 数据库链接：

    KEGG: ag:CAZ39946