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Recombinant Lipoprotein mxiM (mxiM)

  • 货号:
    CSB-YP358133SZB
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 货号:
    CSB-EP358133SZB
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 货号:
    CSB-EP358133SZB-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 货号:
    CSB-BP358133SZB
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 货号:
    CSB-MP358133SZB
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

产品详情

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
    mxiM
  • Uniprot No.:
  • 别名:
    mxiM; sctG; CP0143; Type 3 secretion system pilotin; Lipoprotein MxiM; MxiD pilot; Secretin pilot protein
  • 种属:
    Shigella flexneri
  • 蛋白长度:
    Full Length of Mature Protein
  • 表达区域:
    24-142
  • 氨基酸序列
    CALKSSS NSEKEWHIVP VSKDYFSIPN DLLWSFNTTN KSINVYSKCI SGKAVYSFNA GKFMGNFNVK EVDGCFMDAQ KIAIDKLFSM LKDGVVLKGN KINDTILIEK DGEVKLKLIR GI
  • 蛋白标签:
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Involved in the synthesis of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. Pilot protein that is required for the proper localization of the secretin MxiD/SctC in the outer membrane. Also influences both MxiD/SctC multimerization and stability. Required for both Ipa translocation and tissue culture cell invasion. Binds lipids.
  • 基因功能参考文献:
    1. The structure of MxiM28-142, the Shigella pilot protein that is essential for the assembly and membrane association of the Shigella secretin, MxiD, was determined at 1.5 A. PMID: 15775974
    2. The mutually exclusive association of lipids and MxiD with MxiM establishes a framework for understanding the role of a pilot in the outer-membrane insertion and multimerization of the secretin ring. PMID: 18940609
  • 亚细胞定位:
    Cell outer membrane; Lipid-anchor; Periplasmic side.
  • 数据库链接:

    KEGG: sfl:CP0143