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  4. Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a

Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a

  • 中文名称：
    Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a,Yeast
  • 货号：
    CSB-YP317615LRA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a,Yeast
  • 货号：
    CSB-EP317615LRA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a,Yeast
  • 货号：
    CSB-BP317615LRA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a,Yeast
  • 货号：
    CSB-MP317615LRA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Dermonecrotic toxin LiSicTox-alphaIA1a; EC 4.6.1.-; Dermonecrotic toxin 1; DT1; LiRecDT1; P1; Phospholipase D; PLD; Sphingomyelin phosphodiesterase D 1; SMD 1; SMase D 1; Sphingomyelinase D 1
  • 种属：
    Loxosceles intermedia (Spider)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-306
  • 氨基酸序列
    AGNR RPIWIMGHMV NAIGQIDEFV NLGANSIETD VSFDDNANPE YTYHGIPCDC GRNCKKYENF NDFLKGLRSA TTPGNSKYQE KLVLVVFDLK TGSLYDNQAN DAGKKLAKNL LQHYWNNGNN GGRAYIVLSI PDLNHYPLIK GFKDQLTKDG HPELMDKVGH DFSGNDDIGD VGKAYKKAGI TGHIWQSDGI TNCLPRGLSR VNAAVANRDS ANGFINKVYY WTVDKRSTTR DALDAGVDGI MTNYPDVITD VLNEAAYKKK FRVATYDENP WVTFKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP317615LRA,have you expressed this protein before? Or have any customers reported any activity?
If you have any recommendations for the active form of the protein, it would be greatly appreciated.

A:
Very nice to receive your inquiry. Sorry, this is a semi-custom protein that we haven't expressed before, so we don't receive any feedback about the activity from other customers.
Generally the more advanced expression system is the protein expressed with, the more likely to be active.
Here is a literature for your reference: http://europepmc.org/articles/PMC3034616

靶点详情

  • 功能：
    Dermonecrotic toxins cleave the phosphodiester linkage between the phosphate and headgroup of certain phospholipids (sphingolipid and lysolipid substrates), forming an alcohol (often choline) and a cyclic phosphate. This toxin acts on sphingomyelin (SM) with high activity. It also acts on lysophosphatidylcholine (LPC), and lyso-platelet activating factor (LPAF, an alkyl-LPC) but not on phosphatidylcholine (PC). It may also act on ceramide phosphoethanolamine (CPE), lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), but not on lysophosphatidylserine (LPS), and lysophosphatidylglycerol (LPG). It acts by transphosphatidylation, releasing exclusively cyclic phosphate products as second products. In vivo, it induces dermonecrosis, vascular permeability, platelet aggregation, inflammatory response, edema and cytotoxicity against renal epithelial cells. It causes direct nephrotoxicity and is directly toxic to liver. It also induces hemolysis in a complement-dependent manner as well as in a complement-independent manner. The hemolysis provoked in a complement-independent manner is composed of several steps. The toxin binds to erythrocyte membranes, hydrolyzes membrane phospholipids (SM and LPC) thus generating metabolism products that cause hemolysis, probably by provoking an increase of calcium inside cells. The calcium influx is due to the opening of L-type calcium channels, since L-type calcium channel blockers inhibit calcium influx. In vivo, is lethal to mice when intraperitoneally injected.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Arthropod phospholipase D family, Class II subfamily
  • 组织特异性：
    Expressed by the venom gland.