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  4. Recombinant MMACHC-like protein (cblc-1)

Recombinant MMACHC-like protein (cblc-1)

  • 货号：
    CSB-YP773576CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP773576CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP773576CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP773576CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP773576CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cblc-1
  • Uniprot No.：
  • 别名：
    cblc-1; ZK546.17; MMACHC-like protein; EC 1.16.1.-; Cobalamin deficiency protein 1; Cyanocobalamin reductase; cyanide-eliminating)
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-272
  • 氨基酸序列
    MVTEMSHAES IKRVVDQKLS SHEGFESHMF KIGSYNEAVG ESSPFALPYD DSTMALLILS TPDMFDVAFR KWVVQKTMDF GSFDEVCEMV SSPIQSFLED RLEIMSEKLR KVEENFEILH DYSMTPQRRP KILMQTCGHV AGAAFYYQPC HFQEDGVTWP PAGRMGPNLK FIGLSLHPIY GGHFAFRSVL IFPNVKIPEF CEKEPRPILT ASEDVRTALE KFNYNWKDSG FRDFGNPTRR YSTTQMEFFG RPVAERWEVL RPWVDGGAKN ID
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, using FAD or FMN as cofactor and NADPH as cosubstrate. Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. Under anaerobic conditions cob(I)alamin is the first product; it is highly reactive and is converted to aquocob(II)alamin in the presence of oxygen. Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.
  • 基因功能参考文献：
    1. The crystal structure of ceCblC provides insights into how architectural differences at the alpha- and beta-faces of cobalamin promote the thiol oxidase activity of ceCblC but mute it in wild-type human CblC. PMID: 28442570
    2. ability of the worm CblC to stabilize the highly reactive cob(I)alamin product of the glutathione transferase reaction PMID: 25369151
    3. These results demonstrate that cCblC is a Btrafficking chaperone of C. elegans catalyzing dealkylation and deglutathionylation via a nucleophilic displacement using the thiolate of GSH. PMID: 25256267
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    MMACHC family
  • 数据库链接：

    KEGG: cel:CELE_ZK546.17

    STRING: 6239.ZK546.17.1

    UniGene: Cel.5548