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  4. Recombinant Minor capsid protein L2 (L2)

Recombinant Minor capsid protein L2 (L2)

  • 货号：
    CSB-YP360960BKJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP360960BKJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP360960BKJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP360960BKJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP360960BKJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    L2
  • Uniprot No.：
  • 别名：
    L2Minor capsid protein L2
  • 种属：
    Bovine papillomavirus type 1
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-469
  • 氨基酸序列
    MSARKRVKRA SAYDLYRTCK QAGTCPPDVI PKVEGDTIAD KILKFGGLAI YLGGLGIGTW STGRVAAGGS PRYTPLRTAG STSSLASIGS RAVTAGTRPS IGAGIPLDTL ETLGALRPGV YEDTVLPEAP AIVTPDAVPA DSGLDALSIG TDSSTETLIT LLEPEGPEDI AVLELQPLDR PTWQVSNAVH QSSAYHAPLQ LQSSIAETSG LENIFVGGSG LGDTGGENIE LTYFGSPRTS TPRSIASKSR GILNWFSKRY YTQVPTEDPE VFSSQTFANP LYEAEPAVLK GPSGRVGLSQ VYKPDTLTTR SGTEVGPQLH VRYSLSTIHE DVEAIPYTVD ENTQGLAFVP LHEEQAGFEE IELDDFSETH RLLPQNTSST PVGSGVRRSL IPTQEFSATR PTGVVTYGSP DTYSASPVTD PDSTSPSLVI DDTTTTPIII IDGHTVDLYS SNYTLHPSLL RKRKKRKHA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.
  • 基因功能参考文献：
    1. The s propose that Smurf2 regulates L2 protein cellular localization and therefore alters L2 protein levels. PMID: 21318310
    2. functions as an adapter between the viral DNA via the C-terminal nuclear localization signal and the Kaps via the N-terminal nuclear localization signal and facilitates nuclear import of the DNA during infection PMID: 15564455
    3. Genetic studies identified a highly conserved L2 domain, DKILK, comprising residues 40 to 44 that mediated BPV1 trafficking through the ER during infection via an interaction with the tSNARE syntaxin 18. PMID: 15890910
  • 亚细胞定位：
    Virion. Host nucleus. Host early endosome. Host Golgi apparatus.
  • 蛋白家族：
    Papillomaviridae L2 protein family
  • 数据库链接：

    KEGG: vg:1489023