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  4. Recombinant Mobilization protein A (mobA)

Recombinant Mobilization protein A (mobA)

  • 货号：
    CSB-YP362046ENL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP362046ENL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP362046ENL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP362046ENL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP362046ENL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    mobA
  • Uniprot No.：
  • 别名：
    mobA; repB; Mobilization protein A; DNA strand transferase) [Includes: DNA relaxase; EC 5.6.2.1; DNA nickase); DNA primase; EC 2.7.7.-)]
  • 种属：
    Escherichia coli
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-709
  • 氨基酸序列
    AIYHLTAKT GSRSGGQSAR AKADYIQREG KYARDMDEVL HAESGHMPEF VERPADYWDA ADLYERANGR LFKEVEFALP VELTLDQQKA LASEFAQHLT GAERLPYTLA IHAGGGENPH CHLMISERIN DGIERPAAQW FKRYNGKTPE KGGAQKTEAL KPKAWLEQTR EAWADHANRA LERAGHDARI DHRTLEAQGI ERLPGVHLGP NVVEMEGRGI RTDRADVALN IDTANAQIID LQEYREAIDH ERNRQSEEIQ RHQRVSGADR TAGPEHGDTG RRSPAGHEPD PAGQRGAGGG VAESPAPDRG GMGGAGQRVA GGSRRGEQRR AERPERVAGV ALEAMANRDA GFHDAYGGAA DRIVALARPD ATDNRGRLDL AALGGPMKND RTLQAIGRQL KAMGCERFDI GVRDATTGQM MNREWSAAEV LQNTPWLKRM NAQGNDVYIR PAEQERHGLV LVDDLSEFDL DDMKAEGREP ALVVETSPKN YQAWVKVADA AGGELRGQIA RTLASEYDAD PASADSRHYG RLAGFTNRKD KHTTRAGYQP WVLLRESKGK TATAGPALVQ QAGQQIEQAQ RQQEKARRLA SLELPERQLS RHRRTALDEY RSEMAGLVKR FGDDLSKCDF IAAQKLASRG RSAEEIGKAM AEASPALAER KPGHEADYIE RTVSKVMGLP SVQLARAELA RAPAPRQRGM DRGGPDFSM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the relaxosome complex that is responsible for plasmid transfer during conjugation. Locally unwinds DNA and catalyzes the cleavage of one of the DNA strands at oriT. The cleaved strand is then transferred through the dedicated type IV secretion apparatus. MobA remains covalently linked at the 5' end of the strand, and once in the recipient cell, it probably catalyzes the rejoining of the two ends of the strand, re-forming the circular plasmid DNA. The primase activity of MobA is essential for the synthesis of primers that will initiate the DNA replication events necessary to form the double-stranded plasmid in the recipient cell.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    MobA/MobL family