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  4. Recombinant Moloney murine leukemia virus Gag polyprotein (gag), partial

Recombinant Moloney murine leukemia virus Gag polyprotein (gag), partial

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  • 中文名称：
    Recombinant Moloney murine leukemia virus Gag polyprotein(gag),partial
  • 货号：
    CSB-EP356017MHKe0
  • 规格：
    ￥2328
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356017MHKe0 could indicate that this peptide derived from E.coli-expressed Moloney murine leukemia virus (isolate Shinnick) (MoMLV) gag.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356017MHKe0 could indicate that this peptide derived from E.coli-expressed Moloney murine leukemia virus (isolate Shinnick) (MoMLV) gag.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    gag; Gag polyprotein; Pr65gag; Core polyprotein) [Cleaved into: Matrix protein p15; MA); RNA-binding phosphoprotein p12; pp12); Capsid protein p30; CA); Nucleocapsid protein p10-Gag; NC-gag)]
  • 种属：
    Moloney murine leukemia virus (isolate Shinnick) (MoMLV)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    57.6kDa
  • 表达区域：
    216-478aa
  • 氨基酸序列
    PLRAGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTEDEQKEKERDRRRHREMSKLL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Like all recombinant proteins, this Recombinant MoMLV gag protein was encoded by recombinant DNA. The recombinant DNA was introduced to a plasmid in which the gene of gag was cloned downstream of a promoter region. When the plasmid was introduced to the cells of E.coli, the E.coli’s own protein synthesis pathways would then result in the expression of the gag protein. And the next step was protein purification. The purity of this recombinant protein is 90%+ determined by SDS-PAGE.

    Gag polyprotein exists in all retroviruses, and is a polyprotein comprised of three major functional domains: matrix (MA), capsid (CA), and nucleocapsid (NC). Prior to maturation and viral budding, the full-length Gag protein plays important roles in viral assembly and genome packaging, topics that have been the focus of several review papers. The full-length bovine leukemia virus (BLV) Gag has an elongated rod-shaped structure that is relatively rigid, with the exception of the linker between the MA and CA domains. Deletion of the MA domain maintains the elongated structure but alters the rate of BLV Gag-facilitated annealing of two complementary nucleic acids. Findings suggested that BLV Gag adopts an extended structure in solution and is more similar to MLV Gag and RSV Gag than HIV-1 Gag.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.; Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.; Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.; Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.; Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.
  • 基因功能参考文献：
    1. The structure of the minimal sequence of the murine leukemia virus p12 protein necessary for the host chromatin binding was elucidated. PMID: 27707926
    2. Phosphorylation requirement of murine leukemia virus p12 virus p12 protein necessary for the host chromatin binding was elucidated. PMID: 27707931
    3. The N-terminal intracellular domain of the glycoGag is responsible for its effect on HIV-1 infectivity. PMID: 24403584
    4. s found that mature Gag proteins MA and TM were bound to the immature core consisting of the Gag polyprotein (PrGag) and viral RNA (vRNA). PMID: 23643491
    5. in presence of nucleocapsid (NC) chaperone, RNA dimerization occurred rapidly through a single structural intermediate; NC interacted primarily with guanosine residues; results show RNA chaperones can simplify RNA folding landscapes by weakening intramolecular interactions involving guanosine PMID: 23470731
    6. selective packaging of dimeric RNA is explained by the nucleic acid binding domain derived from Gag binding within the virus to the sequence UCUG-UR-UCUG PMID: 20974908
    7. results suggest a cooperative effect of p12 and capsid during the early events of MLV replication PMID: 15767417
    8. interaction of Gag with Tsg101 and Alix favors budding from the plasma membrane and relieves a requirement for ubiquitination by Nedd4 PMID: 15908698
    9. results indicate that glycosylated gag protein is important for M-MuLV replication in vivo and in vitro and that the protein may be involved in a late step in viral budding or release PMID: 17267509
    10. Abl protein contribute to proper localization by playing a dominant role in trafficking of the v-Abl molecule PMID: 18367522
    11. Basal budding and replication of the murine leukemia virus are independent of the gag L domains PMID: 18667521

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  • 亚细胞定位：
    [Gag polyprotein]: Virion. Host cell membrane; Lipid-anchor. Host late endosome membrane; Lipid-anchor. Host endosome, host multivesicular body.; [Matrix protein p15]: Virion.; [Capsid protein p30]: Virion.; [Nucleocapsid protein p10-Gag]: Virion.; [RNA-binding phosphoprotein p12]: Host cytoplasm.
  • 数据库链接：

    KEGG: vg:1491870