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  4. Recombinant Mouse 2-5A-dependent ribonuclease (Rnasel), partial

Recombinant Mouse 2-5A-dependent ribonuclease (Rnasel), partial

  • 货号：
    CSB-YP313237MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP313237MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP313237MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP313237MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP313237MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rnasel; Rns4; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; EC 3.1.26.-; Ribonuclease 4; Ribonuclease L; RNase L
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover. Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.
  • 基因功能参考文献：
    1. Pref-1 mRNA is a novel substrate of RNase-L. PMID: 27831565
    2. RNAse L stimulates fibroblast migration. PMID: 26517238
    3. Thus, activation of RNase L does not require mouse hepatitis virus virus-induced interferon but rather correlates with adequate levels of basal Oas gene expression, maintained by basal interferon signaling. PMID: 26739051
    4. Thus, RNA cleavage events catalyzed by RNase L are required for optimal inflammasome activation during viral infections. PMID: 25816776
    5. cellular functions of RNase L through protein-protein interactions in the spleen for immune response in mammals PMID: 24780566
    6. Data indicate that deficiency of 2-5A-dependent RNase L (RNase L) in resulted in a significant delay of diabetes onset. PMID: 25287058
    7. RNase L contributes to innate immunity through regulating macrophage functions. PMID: 24324683
    8. By targeting the effector enzyme of this antiviral pathway, L* potently inhibits RNase L, underscoring the importance of this enzyme in innate immunity against Theiler's virus. PMID: 23825954
    9. RNase-L deficiency exacerbates experimental colitis and colitis-associated cancer. PMID: 23567782
    10. These studies highlight novel roles of RNase L in cigarette smoke plus virus induced inflammation, tissue remodeling, apoptosis, and cytokine elaboration PMID: 23913960
    11. RNase L activation during mouse hepatitis virus infection is cell type specific and correlates with relatively high levels of expression of oligoadenylate synthetase genes, which are necessary for an effective RNase L antiviral response. PMID: 23698313
    12. The results suggest that RNase L is not an evolutionarily-conserved host defense mechanism to counteract retroviruses in vivo. PMID: 23725696
    13. These findings highlight RNase L as an essential regulator of adipogenesis via the regulation of CHOP10 mRNA. PMID: 22441668
    14. RNase L induces autophagy via c-Jun N-terminal kinase and double-stranded RNA-dependent protein kinase signaling pathways PMID: 23109342
    15. results indicate a novel function of RNase L as an inducer of autophagy that affects viral yields PMID: 22875977
    16. Mouse hepatitis virus Ns2 cleaves 2',5'-oligoadenylate, the product of 2',5'-oligoadenylate synthetase, to prevent activation of the cellular endoribonuclease RNase L and consequently block viral RNA degradation. PMID: 22704621
    17. sunitinib treatments prevent antiviral innate immune responses mediated by RNase L and PKR. PMID: 21636578
    18. Endoribonuclease L (RNase L) regulates the myogenic and adipogenic potential of myogenic cells PMID: 19851509
    19. JNK and RNase L function in an integrated signaling pathway during the IFN response that leads to elimination of virus-infected cells through apoptosis PMID: 14570908
    20. results indicate that the RNase-L-dependent decrease in mtDNA-encoded mRNA transcript levels occurs through a decrease in the half-life of mt-mRNA, and that RNase-L may play a role in the stability of mt-mRNA PMID: 15522195
    21. The RNase L pathway is critical for in vitro interferon-alpha-mediated islet cell resistance from coxsackievirus serotype B4 infection and replication. PMID: 15661870
    22. RNAse L is required for the antiviral activity of the interferon (IFN)-beta transgene during acute ocular herpes simplex virus type 1 infection. PMID: 16002711
    23. The data indicate that West Nile Virus genomic RNA is susceptible to RNase L cleavage and that RNase L plays a role in the cellular antiviral response to flaviviruses. PMID: 16501108
    24. Data suggest that the primary role of double-stranded RNA binding by the NS1A protein in virus-infected cells is to sequester dsRNA away from RNAse L. PMID: 16627618
    25. Restriction mapping and structure of the 5' flanking region of the RNase L gene. PMID: 17091337
    26. Plays role in senescence that may contribute to its tumor suppressive function and to the enhanced longevity of RNase-L-deficient mice. PMID: 17130839
    27. RNase L does not play a significant role in control of HSV-2 infection in vivo PMID: 17157346
    28. results indicate that RNase L has an essential role in the innate antiviral immune response that relieves the requirement for direct sensing of non-self RNA PMID: 17653195
    29. a unique role for RNase-L in the antibacterial response that is mediated through multiple mechanisms PMID: 19075243
    30. support a model in which the RNase-L-mediated degradation of ribosomal protein mRNAs inhibits translation, and may contribute to its antiproliferative, senescence inducing and tumor suppressor activities. PMID: 19411840
    31. These data demonstrate a novel protective role for RNase L in viral induced CNS encephalomyelitis PMID: 19798426

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  • 亚细胞定位：
    Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Protein kinase superfamily
  • 组织特异性：
    Expressed in spleen, thymus, lung, testis, kidney, liver and heart.
  • 数据库链接：

    KEGG: mmu:24014

    STRING: 10090.ENSMUSP00000083385

    UniGene: Mm.259254