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  4. Recombinant Mouse 5-hydroxytryptamine receptor 2C (Htr2c), partial

Recombinant Mouse 5-hydroxytryptamine receptor 2C (Htr2c), partial

  • 货号：
    CSB-YP010889MO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010889MO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010889MO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010889MO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010889MO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Htr2c; 5ht1c; Htr1c; 5-hydroxytryptamine receptor 2C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-hydroxytryptamine receptor 1C; 5-HT-1C; 5-HT1C; Serotonin receptor 2C
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and feeding behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.
  • 基因功能参考文献：
    1. cortisol up-regulated the expression and function of two serotonin (S) receptors, HTR2c and HTR5a. PMID: 30097291
    2. Activation of the subpopulation of 5-HTCR neurons within the ventral tegmental area is sufficient to reduce homeostatic feeding. PMID: 27882999
    3. Data suggest that Htr2c and leptin in hypothalamic nuclei may be involved in the effects of corticosterone on food intake/appetite regulation. (Htr2c = 5-hydroxytryptamine type 2C receptor) PMID: 28186389
    4. The constitutive activity of 5HT2C is decreased by pre-mRNA editing as well as alternative pre-mRNA splicing, which generates a truncated isoform that switches off 5HT2C receptor activity through heterodimerization; showing that RNA processing regulates the constitutive activity of the 5HT2C system. PMID: 28664341
    5. These data provide new insight into the significance of Htr2c pre-mRNA processing to the physiological regulation of appetite and potentially the pathological manifestation of hyperphagia in Prader-Willi syndrome. PMID: 27931246
    6. olanzapine exerts some of its untoward metabolic effects via antagonism of HTR2C PMID: 28805659
    7. 5HTR2C regulates neurite growth and RGC activity and is necessary for normal amplitude of RGC response to physiologic stimuli. PMID: 26999672
    8. Study identified the 5-hydroxytryptamine (5-HT) 2C receptor (5-HT2CR) population in DA neurons as one potential target for antibinge therapies, and provided preclinical evidence that 5-HT2CR agonists could be used to treat binge eating. PMID: 27516377
    9. Both dorsal raphe serotonergic activity during aversive reinforcement and amygdala serotonin 2C receptor (5-HT2CR) activity during memory consolidation were necessary for stress enhancement of fear memory, but neither process affected fear memory in unstressed mice. Prior stress increased amygdala sensitivity to serotonin by promoting 5-HT2CR surface expression without affecting tissue levels of serotonin in the amygdala. PMID: 26248536
    10. Social Behavioral Deficits Coincide with the Onset of Seizure Susceptibility in Mice Lacking Serotonin Receptor 2c. PMID: 26308619
    11. Study demonstrates that the GHS-R1a/5-HT2C receptor interaction translates into a biologically significant modulation of ghrelin's orexigenic effect PMID: 25727097
    12. This study showed that reduced expression of the 5ht1c which modulate antidepressant action in hippocampus. PMID: 25784603
    13. Suppressed feeding behavior in novelty stress-exposed aged male mice may be mediated by 5-HT(2C)R hypersensitivity, leading to hypoghrelinemia. The hypersensitivity may partly be due to estrogen receptor activation in aged male mice. PMID: 25732068
    14. Results show that mice solely expressing the unedited INI form of 5-HT2C receptors exhibit a hyperactive HPA axis driven by increased CRH, a normal response to chronic stress and decreased depressive-like behaviors and fear-associated memory PMID: 25257581
    15. Chronic intermittent ethanol upregulates 5HT2C receptor signaling in the bed nucleus of the stria terminalis. PMID: 25229718
    16. findings show that enhanced alcohol drinking behaviour in mice is associated with the degree of 5-HT(2C)R mRNA editing in the nucleus accumbens and dorsal raphe nuceus PMID: 24345557
    17. Mice lacking Htr2c specifically in POMC neurons had normal weight but developed glucoregulatory defects: hyperinsulinemia, hyperglucagonemia, hyperglycemia, & insulin resistance. These neurons are needed to control energy & glucose homeostasis. PMID: 24177424
    18. Impaired 5-HT2C receptor signaling during development may predispose to executive function disorders. PMID: 23303047
    19. These experiments thus provided a functional link between 5-HTR2c editing, the splicing process, and the regulation of 5-HTR2c density and function, possibly accounting for the behavioral changes in VGV mice. PMID: 23247076
    20. The findings indicate a role for 5-HT2C receptor-mediated neurotransmission in the expression of this form of impulsive behaviour but suggest that the effects are observed only under acute and reversible changes in 5-HT2C receptor function PMID: 23192316
    21. Data suggest that increased expression of 5-HT(2C)R in pancreatic beta-cells might inhibit insulin secretion. PMID: 23349838
    22. our data suggest that 5-HT(2C)R and leptin receptors are expressed by distinct subpopulations of arcuate POMC neurons and that both 5-HT and leptin exert their actions in POMC neurons via TRPC channels PMID: 21835345
    23. Data suggest that 5HT2c receptors are important elements in generation of hippocampal oscillations (i.e., theta rhythm mediated by serotonin); studies involve freely-moving, sleeping (REM sleep), and anesthetized rats. PMID: 21281651
    24. Mice had high cortical 5-HT2A, but low 5-HT2C receptor densities PMID: 21340474
    25. the 5-HT2CR in the amygdala of SERT-/- mice has increased RNA editing, which could explain, at least in part, the decreased behavioral responses to 5-HT2C agonists in SERT-/- mice. PMID: 21473759
    26. These data constitute the first in vivo demonstration of a role for 5-HT(2C)R mRNA editing in anxiety- and depression-related behaviors. PMID: 20624407
    27. Data suggest that 5-HT2CRs gate food anticipatory activity through mechanisms involving extrahypothalamic neural pathways. PMID: 20668550
    28. Activation of phospholipase C and alternative splicing of pre-mRNA encoding the editing enzymes ADAR1 and ADAR2 have both overlapping and specific roles in modulating 5-HT2CR editing phenotypes. PMID: 20651031
    29. data demonstrate the presence of functional 5-HT(2C) receptors on alveolar macrophages (AM) and suggest a role of 5-HT as novel modulator of AM function. These effects are exclusively driven by the 5-HT(2C) receptor PMID: 20495077
    30. Using a high-throughput multiplexed transcript analysis, we were able to quantify accurately the expression of twenty 5HT(2C) isoforms, each representing at least 0.25% of the total 5HT(2C) transcripts PMID: 20181818
    31. Conclude that the head-twitch response to dopamine receptor partial agonists in mice is strongly modulated by 5-HT(2C) receptor activity. PMID: 20165943
    32. role in altering expression of genes involved in energy expenditure PMID: 12150939
    33. Serotonin 2c receptor knockout mice are more awake and display several abnormalities in rapid eye movement sleep expression and an enhanced response to sleep deprivation compared with wild-type control mice. PMID: 12431861
    34. Modulation of serotonin 2C receptor RNA editing by sustained changes in serotonergic neurotransmission. PMID: 12486144
    35. 5-HT1B receptor knockout mice show a compensatory reduction in 5-HT2C receptor function. PMID: 12534984
    36. hyperactivity and reduced energy cost of physical activity when this gene is mutated or knocked out PMID: 12540602
    37. 5-HT2C R interaction with MUPP1 is dynamically regulated by phosphorylation at Ser458 PMID: 12682077
    38. characterization of several distinct, highly organized behaviors in mice lacking functional 5-HT(2C) receptors, which supports a compulsive-like syndrome PMID: 12782219
    39. These findings illustrate that 5-HT2C pre-mRNA editing responses to stress and chronic fluoxetine in mice are modulated by the genetic background, as well as the behavioral state of the animal. PMID: 15659601
    40. differences between strains were found in total editing frequency, in the proportion of transcripts with 1 and 4 edited sites, in editing frequency at the A, B, E and D sites, in amino acid frequencies at positions 157 and 161, and in protein isoforms. PMID: 16904273
    41. Data implicate responses to spatial learning and stress, in addition to stochastic processes, in the generation of subtle changes in editing patterns of Htr2c. PMID: 17307311
    42. epitope mapping of the 5HT2c receptor PMID: 17360519
    43. The 5-HT2CR protein was found to be co-localized with the GABA synthetic enzyme glutamic acid decarboxylase (GAD), confirming the presence of the 5-HT2CR on GABA neurons within the VTA. PMID: 17367945
    44. 5-HT(2C)R KO mice display a selective blunting of extended amygdala corticotropin-releasing hormone neuronal activation in response to anxiety stimuli. PMID: 17451451
    45. The basolateral amygdala may have a role in maintaining response in the appetitive phase of the second-order schedule and also be susceptible to serotonergic modulation through activation of 5-HT(2C) receptors. PMID: 17561825
    46. Supporting the importance of 5-HT(2C)Rs in CRH neuronal activity, genetic inactivation of 5-HT(2C)Rs produced a downregulation of CRH mRNA and blunted CRH and corticosterone release after 5-HT compound administration. PMID: 17596444
    47. the 5-HT(2C) receptor is involved in modulating contact allergy in mice PMID: 17620091
    48. 5-HT(2C)Rs are coexpressed with neurons containing proopiomelanocortin, known to potently affect appetite, in the arcuate nucleus of the hypothalamus of the mouse PMID: 18039773
    49. Serotonin 2C receptor has direct effects on glucose homeostasis. PMID: 18039786
    50. Mpdz's effects on CNS hyperexcitability, including alcohol and barbiturate withdrawal, involve MPDZ interaction with 5-HT2C and/or GABAB receptors. PMID: 18262506

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Detected in brain cortex, hypothalamus, brainstem and arcuate nucleus. Detected in the paraventricular nucleus of the hypothalamus.
  • 数据库链接：

    KEGG: mmu:15560

    STRING: 10090.ENSMUSP00000043936

    UniGene: Mm.439670