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  4. Recombinant Mouse 7,8-dihydro-8-oxoguanine triphosphatase (Nudt1)

Recombinant Mouse 7,8-dihydro-8-oxoguanine triphosphatase (Nudt1)

  • 中文名称：
    小鼠Nudt1重组蛋白
  • 货号：
    CSB-YP016154MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nudt1重组蛋白
  • 货号：
    CSB-EP016154MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nudt1重组蛋白
  • 货号：
    CSB-EP016154MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nudt1重组蛋白
  • 货号：
    CSB-BP016154MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nudt1重组蛋白
  • 货号：
    CSB-MP016154MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nudt1
  • Uniprot No.：
  • 别名：
    Nudt1; Mth17,8-dihydro-8-oxoguanine triphosphatase; EC 3.6.1.55; 2-hydroxy-dATP diphosphatase; EC 3.6.1.56; 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    21-156
  • 氨基酸序列
    GMKKRGFGAG RWNGFGGKVQ EGETIEDGAK RELLEESGLS VDTLHKVGHI SFEFVGSPEL MDVHIFSADH VHGTPTESEE MRPQWFQLDQ IPFADLWPDD SYWFPLLLQK KKFCGHFKFQ DQDTILSYSL REVDSF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.
  • 基因功能参考文献：
    1. Increased cytotoxicity following MTH1 knockout or treatment with MTH1 inhibitor suggests the oxidation of precursor nucleotides. PMID: 28067232
    2. The crystal structures of mouse MTH1 in complex with inhibitor TH588 and dog MTH1 elucidate the structural and sequence basis for the observed difference in their affinities for TH588. Amino acid residue 116 in MTH1 is an important determinant of TH588 affinity. The structure of mouse MTH1 in complex with the substrate 8-oxo-dGTP is presented. PMID: 29281266
    3. These results suggest that MTH1 deficiency might be a causative factor for aging and age-related disorders. PMID: 21538080
    4. MTH1 protects cells from H2O2-induced cell dysfunction and death by hydrolyzing oxidized purine nucleotides including 8-oxo-dGTP and 2-OH-dATP. PMID: 12857738
    5. MTH1 efficiently suppresses the accumulation of 8-oxoG in both cellular DNA and RNA in the hippocampus, especially in microglia, caused by excitotoxicity. PMID: 16467516
    6. An age-related higher level of 8-oxodG even occurs concomitantly with high 8-oxodGTPase activity. PMID: 19500668

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus membrane. Cytoplasmic vesicle, secretory vesicle, acrosome.
  • 蛋白家族：
    Nudix hydrolase family
  • 组织特异性：
    High expression levels detected in thymus, liver, spleen, kidney, testis and large intestine, with lower levels detected in brain, heart, lung and stomach (at protein level). Expressed in kidney, liver and small intestine.
  • 数据库链接：

    KEGG: mmu:17766

    STRING: 10090.ENSMUSP00000059983

    UniGene: Mm.118846