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  4. Recombinant Mouse 72 kDa inositol polyphosphate 5-phosphatase (Inpp5e)

Recombinant Mouse 72 kDa inositol polyphosphate 5-phosphatase (Inpp5e)

  • 货号：
    CSB-YP872841MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP872841MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP872841MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP872841MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP872841MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Inpp5e; Phosphatidylinositol polyphosphate 5-phosphatase type IV; 72 kDa inositol polyphosphate 5-phosphatase; Inositol polyphosphate-5-phosphatase E; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; EC 3.1.3.36; Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase; EC 3.1.3.86
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-647
  • 氨基酸序列
    MPSKSACLRH TEAPGQLEGR MLQGQPPNTE KKLIPTPGFL PASDSQGSET NPMPPFSIPA KTSNQNPQTK ANLITPQPPI RPKLERTLSL DDKGWRRRRF RGSQEDLTVQ NGASPCRGSL QDSVAQSPAY SRPLPCLSTS LQEIPKSRRA TGSEGGSPSL WSDCLSGMIS TSLDLLHRDA ASGGPPSRLA SLHASHTPPA MDLSIASSSL RTANKVDPEH TDYKLRMQTR LVRAHSNLGP SRPRSPLAGD DHSIHSARSF SLLAPIRTKD IRSRSYLEGS LLASGALLGA EELARYFPDR NMALFVATWN MQGQKELPAS LDEFLLPTEA DYTQDLYVIG IQEGCSDRRE WETRLQETLG PQYVLLSSAA HGVLYMSLFI RRDLIWFCSE VEYSTVTTRI VSQIKTKGAL GVSFTFFGTS FLFITSHFTS GDGKVAERLL DYSRTIQALA LPRNVPDTNP YRSSAGDVTT RFDEVFWFGD FNFRLSGGRV AVEAFLKQKP EVDVLALLQH DQLTREMKKG SIFRGFEEAE IHFLPSYKFD IGKDTYDSTS KQRTPSYTDR VLYKSRHKGD ICPMKYSSCP GIKTSDHRPV YGLFQVKVRP GRDNIPLAAG KFDRELYLIG IKRRISKEIQ RQEALKSQSS SAVCTVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Specific for lipid substrates, inactive towards water soluble inositol phosphates. Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability.
  • 基因功能参考文献：
    1. It postulates a three-step mechanism regulating ciliary localization of INPP5E, consisting of farnesylation- and PDE6delta-mediated targeting, INPP5E-PDE6delta complex diffusion into the cilium with transfer to the IFT system, and retention inside cilia. PMID: 29140789
    2. The expression levels of the INPP5E gene in NTD mouse embryos were significantly lower compared with control embryos, at the time of neural tube closure (gestational day 11.5). The INPP5E gene regulates the process of embryonic neural development. Abnormal levels of expression of the INPP5E gene may contribute to NTDs. PMID: 29626185
    3. we identify Inpp5e as an essential inhibitor of the PI3K/Akt/mTORC1 signaling axis in renal epithelial cells, and demonstrate a critical role for Inpp5e-dependent mTORC1 regulation in Polycystic kidney disease (PKD) suppression PMID: 27056978
    4. INPP5E localizes to centrosomes, chromosomes, and kinetochores in early mitosis and shuttles to the midzone spindle at mitotic exit. PMID: 28031327
    5. INPP5E is an essential point of convergence between Hedgehog and phosphoinositide signaling at cilia that maintains transition zone function and Hedgehog-dependent embryonic development. PMID: 27998989
    6. the affinity to PDE6delta and the release by Arl2/3 in addition to a retention signal are the determinants for cargo sorting and enrichment at its destination. PMID: 27063844
    7. PIPKIgamma and INPP5E localize to the centrosome and coordinate the initiation of ciliogenesis. PMID: 26916822
    8. Inpp5e, through functional interactions with Rab20 on the phagosome, activates Rab5, which, in turn, increases PtdIns3P and delays phagosome acidification. PMID: 25269936
    9. Inpp5e and SHIP1 exhibit specificity in regulating FcgammaR- versus CR3-stimulated phagocytosis by controlling the amplitude and duration of PtdIns(3,4,5)P(3) at the phagocytic cup. PMID: 17682126
    10. Inpp5e mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in the mouse. PMID: 19668215

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, cilium axoneme. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Nucleus.
  • 蛋白家族：
    Inositol 1,4,5-trisphosphate 5-phosphatase type IV family
  • 组织特异性：
    Highly expressed in testis, in pachytene and diplotene spermatocytes, but not in more mature elongating spermatids. Detected in neurons throughout the brain.
  • 数据库链接：

    KEGG: mmu:64436

    STRING: 10090.ENSMUSP00000119485

    UniGene: Mm.330070