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  4. Recombinant Mouse Acetoacetyl-CoA synthetase (Aacs)

Recombinant Mouse Acetoacetyl-CoA synthetase (Aacs)

  • 货号：
    CSB-YP001012MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001012MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001012MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001012MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001012MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Aacs
  • Uniprot No.：
  • 别名：
    AacsAcetoacetyl-CoA synthetase; EC 6.2.1.16
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-672
  • 氨基酸序列
    MSKLARLERE EIMECQVMWE PDSKKDTQMD RFRAAVGTAC GLALGNYNDL YHWSVRSYMD FWAEFWKFSG IVYSRMYDEV VDTSKGIADV PEWFRGSRLN YAENLLRHKE NDRVALYVAR EGREEIVKVT FEELRQQVAL FAAAMRKMGV KKGDRVVGYL PNSAHAVEAM LAAASIGAIW SSTSPDFGVN GVLDRFSQIQ PKLIFSVEAV VYNGKEHGHL EKLQRVVKGL PDLQRVVLIP YVLPREKIDI SKIPNSVFLD DFLASGTGAQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM VHSAGGTLIQ HLKEHMLHGN MTSSDILLYY TTVGWMMWNW MVSALATGAS LVLYDGSPLV PTPNVLWDLV DRIGITILGT GAKWLSVLEE KDMKPVETHN LHTLHTILST GSPLKAQSYE YVYRCIKSSV LLGSISGGTD IISCFMGQNS SIPVYKGEIQ ARNLGMAVEA WDEEGKAVWG ASGELVCTKP IPCQPTHFWN DENGSKYRKA YFSKFPGVWA HGDYCRINPK TGGIIMLGRS DGTLNPNGVR FGSSEIYNIV EAFDEVEDSL CVPQYNRDGE ERVVLFLKMA SGHTFQPDLV KRIRDAIRLG LSARHVPSLI LETRGIPYTL NGKKVEVAVK QVMAGRTVEH RGAFSNPETL DLYRDIPELQ DF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
  • 基因功能参考文献：
    1. These results suggest that Sp1 regulates gene expression of AACS in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation. PMID: 29137983
    2. Cleavage of AACS by legumain is critical for the regulation of enzymatic activity and results in gain-of-function changes. PMID: 27129883
    3. Knockdown of AACS inhibits differentiation of 3T3-L1 adipocytes and suppresses expression of the adipocyte markers, peroxisome proliferator-activated receptor gama and CCAAT/enhancer binding protein alpha. PMID: 23123469
    4. Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels. PMID: 22985732
    5. These results suggest that AACS is regulated by SREBP-2 and involves in the normal development of neurons. PMID: 23000407
    6. AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. PMID: 18514076

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    ATP-dependent AMP-binding enzyme family
  • 数据库链接：

    KEGG: mmu:78894

    STRING: 10090.ENSMUSP00000031445

    UniGene: Mm.431573