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  4. Recombinant Mouse Acidic mammalian chitinase (Chia)

Recombinant Mouse Acidic mammalian chitinase (Chia)

  • 中文名称：
    Recombinant Mouse Acidic mammalian chitinase(Chia),Yeast
  • 货号：
    CSB-YP838694MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Acidic mammalian chitinase(Chia),Yeast
  • 货号：
    CSB-EP838694MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Acidic mammalian chitinase(Chia),Yeast
  • 货号：
    CSB-EP838694MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Acidic mammalian chitinase(Chia),Yeast
  • 货号：
    CSB-BP838694MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Acidic mammalian chitinase(Chia),Yeast
  • 货号：
    CSB-MP838694MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Chia
  • Uniprot No.：
  • 别名：
    Chia; Chia1; Acidic mammalian chitinase; AMCase; EC 3.2.1.14; YNL
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-473
  • 氨基酸序列
    YNLICYFTN WAQYRPGLGS FKPDDINPCL CTHLIYAFAG MQNNEITTIE WNDVTLYKAF NDLKNRNSKL KTLLAIGGWN FGTAPFTTMV STSQNRQTFI TSVIKFLRQY GFDGLDLDWE YPGSRGSPPQ DKHLFTVLVK EMREAFEQEA IESNRPRLMV TAAVAGGISN IQAGYEIPEL SKYLDFIHVM TYDLHGSWEG YTGENSPLYK YPTETGSNAY LNVDYVMNYW KNNGAPAEKL IVGFPEYGHT FILRNPSDNG IGAPTSGDGP AGPYTRQAGF WAYYEICTFL RSGATEVWDA SQEVPYAYKA NEWLGYDNIK SFSVKAQWLK QNNFGGAMIW AIDLDDFTGS FCDQGKFPLT STLNKALGIS TEGCTAPDVP SEPVTTPPGS GSGGGSSGGS SGGSGFCADK ADGLYPVADD RNAFWQCING ITYQQHCQAG LVFDTSCNCC NWP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP838694MO, could you please provide some informations for all 4 expression hosts?
Also, regarding the purity, what are the potential contaminants that could be retained in the final product?

A:
Very nice to receive your inquiry. Recombinant Mouse Acidic mammalian chitinase(Chia)
CSB-YP838694MO >> Yeast
CSB-EP838694MO >> E.coli
CSB-BP838694MO >> Baculovirus
CSB-MP838694MO >> Mammalian cell
Expression Region: 22-473aa; Full length of the mature protein.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

YNLICYFTNWAQYRPGLGSFKPDDINPCLCTHLIYAFAGMQNNEITTIEWNDVTLYKAFNDLKNRNSKLKTLLAIGGWNFGTAPFTTMVSTSQNRQTFITSVIKFLRQYGFDGLDLDWEYPGSRGSPPQDKHLFTVLVKEMREAFEQEAIESNRPRLMVTAAVAGGISNIQAGYEIPELSKYLDFIHVMTYDLHGSWEGYTGENSPLYKYPTETGSNAYLNVDYVMNYWKNNGAPAEKLIVGFPEYGHTFILRNPSDNGIGAPTSGDGPAGPYTRQAGFWAYYEICTFLRSGATEVWDASQEVPYAYKANEWLGYDNIKSFSVKAQWLKQNNFGGAMIWAIDLDDFTGSFCDQGKFPLTSTLNKALGISTEGCTAPDVPSEPVTTPPGSGSGGGSSGGSSGGSGFCADKADGLYPVADDRNAFWQCINGITYQQHCQAGLVFDTSCNCCNWP


We can guarantee that the final purity will reach more than 85%, the final product may contain a small amount of host cell self protein.

靶点详情

  • 功能：
    Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Presence in saliva and gastric juice suggests a function as a digestive enzyme.
  • 基因功能参考文献：
    1. The results indicate that mouse AMCase catalyzes hydrolysis as well as transglycosylation and suggest that this enzyme can play a novel role under physiological conditions in peripheral tissues, such as the lungs. PMID: 28833103
    2. we showed that M at the position 61 is highly conserved in the many mammals other than orangutan and humans. In addition, introduction of M61R and M61I to WT mouse AMCase lead to a significant reduction of its chitinolytic activity. Taken together, our present and previous data by Seibold et al. (2009) indicate that G339T (R61M) in humans is associated with asthma protection in the fashion of gain of function PMID: 27702777
    3. AMCase-deficient mice exhibit premature morbidity and mortality, concomitant with accumulation of environmentally derived chitin polymers in the airways and expression of pro-fibrotic cytokines. Over time, these mice develop spontaneous pulmonary fibrosis. PMID: 28431248
    4. Data show that acidic mammalian chitinase (AMCase) functions as a critical initiator of protective type 2 responses to intestinal nematodes, but is largely dispensable for allergic responses in the lung. PMID: 27043413
    5. AMCase expression was significantly decreased in experimental autoimmune encephalitis mice compared to PBS-controls. PMID: 26079949
    6. Protein A-mouse acidic mammalian chitinase-V5-His expressed in periplasmic space of Escherichia coli possesses chitinase functions comparable to CHO-expressed protein. PMID: 24244337
    7. AMCase mRNA is a major transcript in mouse stomach acting as a digestive enzyme that breaks down polymeric chitin and as part of the host defense against chitin-containing pathogens in the gastric contents. PMID: 23185612
    8. Although exposure to allergen stimulates the expression of AMCase and increased chitinolytic activity in murine airways, the overexpression or inhibition of AMCase exerts only a subtle impact on AAD. PMID: 21836154
    9. findings show that acidic mammalian chitinase is induced via a T helper-2 (Th2)-specific, interleukin-13 (IL-13)-mediated pathway in epithelial cells and macrophages in an aeroallergen asthma model PMID: 15192232
    10. AMCase provides the first positive molecular marker of distal Clara cell secretory protein-expressing cells under these conditions. PMID: 16556727
    11. lung epithelial cells secrete AMCase via an EGFR-dependent pathway that is activated by ADAM17 and mediates its effects via Ras. PMID: 18824549

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  • 亚细胞定位：
    Secreted. Cytoplasm. Cytoplasmic granule. Note=Detected in secretory granules of parotid acinar cells and gastric chief cells and secreted from them into saliva and gastric juice, respectively.
  • 蛋白家族：
    Glycosyl hydrolase 18 family, Chitinase class II subfamily
  • 组织特异性：
    Detected in macrophages and lung epithelial cells. Detected in the acinar cells of parotid gland and von Ebner's gland but not in submandibular and sublingual glands. Detected in gastric chief cells. Also present in parotid glandular saliva and gastric ju
  • 数据库链接：

    KEGG: mmu:81600

    STRING: 10090.ENSMUSP00000078134

    UniGene: Mm.46418