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  4. Recombinant Mouse Aldo-keto reductase family 1 member C21 (Akr1c21)

Recombinant Mouse Aldo-keto reductase family 1 member C21 (Akr1c21)

  • 中文名称：
    Recombinant Mouse Aldo-keto reductase family 1 member C21(Akr1c21),Yeast
  • 货号：
    CSB-YP845990MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Aldo-keto reductase family 1 member C21(Akr1c21),Yeast
  • 货号：
    CSB-EP845990MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Aldo-keto reductase family 1 member C21(Akr1c21),Yeast
  • 货号：
    CSB-EP845990MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Aldo-keto reductase family 1 member C21(Akr1c21),Yeast
  • 货号：
    CSB-BP845990MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Aldo-keto reductase family 1 member C21(Akr1c21),Yeast
  • 货号：
    CSB-MP845990MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Akr1c21
  • Uniprot No.：
  • 别名：
    Akr1c21; Aldo-keto reductase family 1 member C21; EC 1.1.1.-; 17-alpha-hydroxysteroid dehydrogenase; 17-alpha-HSD; 3(or 17)-alpha-hydroxysteroid dehydrogenase; EC 1.1.1.209; 3-alpha-hydroxysteroid dehydrogenase; Dihydrodiol dehydrogenase type 1; DD1; Dihydrodiol dehydrogenase type 3; DD3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-323
  • 氨基酸序列
    MNSKCHCVIL NDGNFIPVLG FGTALPLECP KSKAKELTKI AIDAGFHHFD SASVYNTEDH VGEAIRSKIA DGTVRREDIF YTSKVWCTSL HPELVRASLE RSLQKLQFDY VDLYLIHYPM ALKPGEENFP VDEHGKLIFD RVDLCATWEA MEKCKDAGLT KSIGVSNFNY RQLEMILNKP GLKYKPVCNQ VECHPYLNQM KLLDFCKSKD IVLVAYGVLG TQRYGGWVDQ NSPVLLDEPV LGSMAKKYNR TPALIALRYQ LQRGIVVLNT SLKEERIKEN MQVFEFQLSS EDMKVLDGLN RNMRYIPAAI FKGHPNWPFL DEY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into androsterone. Has lower 3-alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha-hydroxysteroids, 17-ketosteroids, 3-alpha hydroxysteroids and 3-ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17-alpha-hydroxysteroids. Likewise, reduction of 3-ketosteroids yields only 3-alpha-hydroxysteroids.
  • 基因功能参考文献：
    1. mutation resulted in a change in the conformation of the flexible loop B, including the V-shaped groove, which is a unique feature of the active-site architecture of wild-type AKR1C21 and is formed by the side chains of Tyr224 and Trp227. PMID: 20124700
    2. X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase PMID: 16511129
    3. crystal structure of the m17alpha-HSD enzyme in its apo-form (1.9 A resolution) as well as those of two different forms of this enzyme in binary complex with NADP(H) (2.9 A and 1.35 A resolution)( PMID: 17034817
    4. study reports a ternary complex crystal structure (m17alpha-HSD:NADP(+):epi-T) determined at 1.85 A resolution reveals a unique steroid-binding mode for an AKR enzyme PMID: 17442338
    5. Gly225 & Gly226 help determine the substrate stereospecificity. The G225P/G226P mutation reduced the affinity for both 3alpha- & 17alpha-hydroxysteroid substrates by up to 160-fold, indicating that these residues are critical for substrate binding. PMID: 19237748

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Aldo/keto reductase family
  • 组织特异性：
    Detected in kidney and brain.
  • 数据库链接：

    KEGG: mmu:77337

    STRING: 10090.ENSMUSP00000021628

    UniGene: Mm.27085