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  4. Recombinant Mouse Amiloride-sensitive sodium channel subunit alpha (Scnn1a), partial

Recombinant Mouse Amiloride-sensitive sodium channel subunit alpha (Scnn1a), partial

  • 中文名称：
    小鼠Scnn1a重组蛋白
  • 货号：
    CSB-YP717201MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Scnn1a重组蛋白
  • 货号：
    CSB-EP717201MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Scnn1a重组蛋白
  • 货号：
    CSB-EP717201MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Scnn1a重组蛋白
  • 货号：
    CSB-BP717201MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Scnn1a重组蛋白
  • 货号：
    CSB-MP717201MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Scnn1a
  • Uniprot No.：
  • 别名：
    Scnn1a; Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
  • 基因功能参考文献：
    1. Increased ENAC alpha, beta and gamma subunits expression is associated with Lung disease. PMID: 29074490
    2. The derlin-1 pathway therefore may represent a significant early checkpoint in the recognition and degradation of ENaC in mammalian cells. PMID: 28137758
    3. nonselective cation channels ( channels consist of at least one alpha-ENaC and one or more acid-sensing ion channel 1a (ASIC1a) proteins. PMID: 28283476
    4. ENaC and ROMK channel activity in kidney tubules are inhibited in TgWnk4(pseudoaldosteronism type II) mice. Wnk4(PHAII)-induced inhibition of ENaC and ROMK may contribute to the suppression of K(+) secretion in the tubules. PMID: 28365586
    5. Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC Activity PMID: 27802315
    6. depletion of Kir4.1 stimulates the expression of ENaCbeta and -gamma subunits in the cortical collecting duct PMID: 26887833
    7. Lack of epithelial sodium channel-mediated sodium transport along the nephron cannot be compensated for by other sodium channels and/or transporters, only by a high-sodium and reduced-potassium diet. PMID: 26701978
    8. LEFTY2 regulates the expression and activity of ENaC in endometrial epithelial cells via SGK1. PMID: 27606670
    9. reducing ENaC expression in the connecting tubule induces clear pseudohypoaldosteronism type 1 symptoms during high dietary potassium loading PMID: 26582762
    10. PCSK9(-/-) mice display normal sodium balance and blood pressure regulation despite an increase of cleaved alphaENaC under basal condition PMID: 25621930
    11. H-Ras mediates the inhibitory effect of epidermal growth factor on the epithelial Na+ channel PMID: 25774517
    12. 17beta-estradiol attenuates LPS-induced acute lung injury via PI3K/Akt/SGK1 mediated up-regulation of Scnn1a. PMID: 25551628
    13. ENaC regulates miR-101/miR-199a-3p during embryo implantation with the involvement of CREB phosphorylation. PMID: 25187622
    14. Pendrin gene ablation reduced ENaC-mediated Na(+) absorption by reducing channel open probability as well as by reducing channel density through changes in subunit total protein abundance and subcellular distribution. PMID: 25972513
    15. results are consistent with a role of alphaENaC in connecting tubule in rosiglitazone-induced fluid retention, which dovetails with the physiological relevance of ENaC in this segment PMID: 25531136
    16. In the absence of Na(+)-HCO3 (-) cotransporter electrogenic 2 in the late distal tubule, acid-loaded mice exhibit disinhibition of ENaC-mediated Na(+) reabsorption, which results in Na(+) retention, K(+) wasting, and hypertension. PMID: 25941340
    17. sustained stimulatory effect of locally elevated norepinephrine on ENaC-mediated Na(+) absorption may contribute to the hypertensive effect of increased renal sympathetic activity PMID: 25520009
    18. mTOR regulates kidney tubule ion handling and suggest that mTOR regulates Na+ homeostasis through SGK1-dependent modulation of ENaC activity. PMID: 25415435
    19. Na+ inhibits the epithelial Na+ channel by binding to a site in an extracellular acidic cleft. PMID: 25389295
    20. Increased CFTR expression and decreased ENaC-alpha expression in the decidua of early abortion may relate with failure of early pregnancy. PMID: 24914548
    21. TIP peptide directly activates ENaC upon binding to the carboxy-terminal domain of the alpha subunit of the channel. In the presence of PLY, a mediator of pneumococcal-induced pulmonary edema, this binding stabilizes the ENaC-PIP2-MARCKS complex. PMID: 25029038
    22. ENaC colonic activity is impaired in gene-targeted mice lacking functional Janus kinase 3 tyrosine-protein kinase (JAK)3. PMID: 24281140
    23. Impaired alphaENaC expression due to failure to inhibit Dot1a-Af9 may play an important role in the early stages of pseudohypoaldosteronism type 1 in MR(-/-) mice. PMID: 24026182
    24. A novel epigenetic mechanism for the control of basal and aldosterone-induced alphaENaC transcription adds to previously described epigenetic controls exerted by histone modifications. PMID: 23926181
    25. ENaC in the vascular endothelium determines cellular mechanics and hence might participate in the control of vascular function. PMID: 23460285
    26. analysis of inhibition of neuronal degenerin/epithelial Na+ channels by the multiple sclerosis drug 4-aminopyridine PMID: 23404498
    27. It was concluded that +78/+92 of the epithelial Na+ channel alpha-subunit represents the primary Af9 binding site involved in recruiting Dot1a to repress basal and aldosterone-sensitive Na+ channel alpha-subunit transcription. PMID: 23152297
    28. These data suggest that adenylyl cyclase VI mediates vasopressin-stimulated ENaC activity in the kidney. PMID: 23264685
    29. Response to shear stress or to Na+ is altered by mutations in the wrist domain of the alpha subunit. PMID: 23144453
    30. ENaC is not a passive passenger in regulated epithelial vesicle trafficking, but plays a role in establishing and maintaining the pool of vesicles that respond to cAMP stimulation. PMID: 23029554
    31. Data indicate that under normal sodium intake, epithelial Na(+) channel (ENaC) open probability (P(o)) was modestly elevated in bradykinin receptors B1R, B2R(-/-) mice. PMID: 23033373
    32. Mutations of the serine protease CAP1/Prss8 lead to reduced embryonic viability, skin defects, and decreased ENaC activity. PMID: 22705055
    33. DEG/ENaC sodium channels are not mechanosensors in mouse sensory neurons. PMID: 22449024
    34. These results indicate a previously undefined role of ENaC in regulating the PGE production and release required for embryo implantation. PMID: 22729284
    35. Aldosterone-independent stimulation by AVP shifts the role of ENaC in the ASDN from protecting Na(+) balance to promoting water reabsorption PMID: 22665796
    36. Results suggest that the finger domain has an important role in the modulation of the results suggest that the finger domain has an important role in the modulation of epithelial sodium channel (ENaC) activity in response to shear stress. PMID: 22408250
    37. AF17 promotes Dot1a nuclear export and upregulates basal, but not aldosterone-stimulated ENaC expression, leading to an increase in ENaC-mediated Na(+) transport in renal collecting duct cells PMID: 22087315
    38. The reactive intermediates formed during and after exposure to chlorine activated ERK1/2 in alveolar epithelial cells, leading to decreased ENaC concentrations and activity. PMID: 21997487
    39. The bulk of endothelin (ET)-1 inhibition of ENaC activity is mediated by the ETB receptor in the kidney collecting duct. PMID: 21918182
    40. Enhancement of alveolar epithelial sodium channel activity is found with decreased cystic fibrosis transmembrane conductance regulator expression in mouse lung. PMID: 21743028
    41. In Reissner's membrane epithelial cells from cochlea, the epithelial sodium channel was found to be a highly Na+-selective channel with characteristics in common with alphabetagamma-ENaC. PMID: 21284860
    42. estrogen regulates expression and function of epithelial sodium channel alpha in osteoblasts PMID: 21624334
    43. Transgenic knockout mice with the alpha-ENaC gene disrupted die soon after birth because of an inability to remove fluid from their lungs. PMID: 21448181
    44. loop has a role in modulating channel gating in response to external stimuli, and external signals trigger movements within the extracellular regions of ENaC subunits that are transmitted to the channel gate. PMID: 21367859
    45. Epithelial sodium channel activity is more sensitive to changes in PO2 than previously thought. PMID: 21123494
    46. epithelial sodium channel-mediated lithium entry into the collecting ducts principal cells contributes to the pathogenesis of lithium-induced nephrogenic diabetes insipidus(NDI). PMID: 21051735
    47. loss of the ability of ENaC to respond to changes in sodium levels contributes to salt-sensitive hypertension in Cx30(-/-) mice PMID: 21075848
    48. molecular models of the extracellular region of alpha ENaC PMID: 20974852
    49. activation of Na-Cl cotransporter is not sufficient by itself to induce a hyperkalemic hypertension and that the deregulation of other channels, such as the Epithelial Na(+) channel (ENaC), is probably required PMID: 20921400
    50. Pendrin modulates ENaC abundance and function within the cortical collecting duct, at least in part by increasing luminal [HCO(3)(-)] and/or pH. PMID: 20966128

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cell projection, cilium. Cytoplasmic granule. Cytoplasm. Cytoplasmic vesicle, secretory vesicle, acrosome. Cell projection, cilium, flagellum.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, SCNN1A subfamily
  • 组织特异性：
    Expressed in kidney (at protein level). Expressed in lung (at protein level). Expressed in the epididymis (at protein level). In the caput and corpus regions of the epididymis, expressed uniformly on the luminal and basal surfaces of the ducts and in the
  • 数据库链接：

    KEGG: mmu:20276

    STRING: 10090.ENSMUSP00000080164

    UniGene: Mm.144114