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  4. Recombinant Mouse Ammonium transporter Rh type C (Rhcg), partial

Recombinant Mouse Ammonium transporter Rh type C (Rhcg), partial

  • 中文名称：
    小鼠Rhcg重组蛋白
  • 货号：
    CSB-YP882548MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rhcg重组蛋白
  • 货号：
    CSB-EP882548MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rhcg重组蛋白
  • 货号：
    CSB-EP882548MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rhcg重组蛋白
  • 货号：
    CSB-BP882548MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rhcg重组蛋白
  • 货号：
    CSB-MP882548MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rhcg
  • Uniprot No.：
  • 别名：
    Rhcg; Ammonium transporter Rh type C; Rhesus blood group family type C glycoprotein; Rh family type C glycoprotein; Rh type C glycoprotein; Rhesus blood group-associated C glycoprotein; Rhesus-associated C glycoprotein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.
  • 基因功能参考文献：
    1. RhCG and H+ATPases are located within the same cellular protein complex in the kidney and this interaction is required for maximal urinary acidification by H+-ATPases, a prerequisite for efficient NH3 secretion and urine excretion of NH4+. PMID: 29054531
    2. We characterized ammonia and ammonium (NH3/NH4 (+)) transport by the rhesus-associated (Rh) glycoproteins RhAG, Rhbg, and Rhcg expressed in Xenopus oocytes. We used ion-selective microelectrodes and two-electrode voltage clamp to measure changes in intracellular pH, surface pH, and whole cell currents induced by NH3/NH4 (+) and methyl amine/ammonium (MA/MA(+)). PMID: 26354748
    3. investigated the renal adaptive response of acid excretory pathways to high-protein diets containing normal or low amounts of acid-producing sulfur amino acids (SAA) and examined how this adaption requires the RhCG ammonia transporter PMID: 24652796
    4. Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) are identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion. PMID: 24647713
    5. Rhcg is critically important in the renal response to metabolic acidosis. in mice with intact Rhcg expression, metabolic acidosis does not induce maximal changes in PEPCK, NHE-3, and GS expression despite the presence of persistent metabolic acidosis. PMID: 24338819
    6. highly expressed in specific cells in the male reproductive tract PMID: 23904565
    7. hypokalemia increases collecting duct Rhcg expression, and this likely contributes to the hypokalemia-stimulated increase in urinary ammonia excretion, and its adaptive increases in phosphate-dependent glutaminase expression can compensate for the absence of collecting duct Rhcg. PMID: 23195675
    8. Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct PMID: 23281477
    9. Rhcg expression in intercalated cells is necessary for the normal renal response to metabolic acidosis, contributing to both basal and acidosis-stimulated ammonia excretion. Adaptations in Rhbg expression occur in response to acid-loading. PMID: 20462967
    10. Localization of the ammonium transporter proteins RhBG and RhCG in mouse kidney. PMID: 12388412
    11. RhBG and RhCG are present in a major site of ammonia secretion in the kidney, i.e., the CNT and CD, in agreement with their putative role in ammonium transport. PMID: 12595489
    12. RhCG immunoreactivity is present in bile duct epithelial cells, where it may contribute to ammonium secretion into bile fluid. PMID: 12730882
    13. The expression of the ammonia transporter family members Rh B glycoprotein (RhBG) and Rh C glycoprotein (RhCG) in the mouse gastrointestinal tract. PMID: 15576624
    14. These results strongly suggest that RhBG and RhCG transport ammonia by an electroneutral process that involves NH4(+)/H+ exchange resulting in net NH3 translocation. PMID: 16131648
    15. RhCG-mediated transport is an electroneutral process that is driven by the NH4+ concentration and the transmembrane H+ gradient, effectively exchanging NH4+ for H+ in a process that results in transport of net NH3. PMID: 16563829
    16. Review. Animal studies show Rhcg has a role in transmembrane NH3 or NH4+ transport in epithelial cells. PMID: 16563831
    17. Review. Renal RhCG belongs to the Mep/Amt family of ammonium transporters & promotes bi-directional ammonium transport upon heterologous expression in yeast. Its role in ammonium excretion is being studied in knockout mice. PMID: 16564721
    18. Review. In kidney, Rhcg immunoreactivity is exclusively apical. Rhcg is present in bile duct epithelia. In the gastrointestinal tract, Rhcg exhibits cell-specific, axially heterogeneous, and polarized expression. PMID: 16564723
    19. RhCG expression enhanced the ammonium-induced initial alkalinization (related to NH3 influx)& secondary acidification (related to NH4+ influx). Sub-millimolar NH4+ concentrations induced inward currents in voltage-clamped RhCG-expressing cells. PMID: 16564724
    20. a critical role for Rhcg in ammonium handling and pH homeostasis both in the kidney and the male reproductive tract PMID: 19020613
    21. Rh C glycoprotein is present in both the apical and basolateral plasma membrane in the mouse kidney, where it is likely to contribute to renal ammonia metabolism. PMID: 19129254
    22. This study concluded that collecting duct Rhcg expression contributes to both basal and acidosis-stimulated renal ammonia excretion, indicating that collecting duct ammonia secretion is, at least in part, mediated by Rhcg and not only lipid diffusion. PMID: 19321595
    23. Rhbg and Rhcg may contribute to bronchial epithelial cell ammonia metabolism and suggest that they do not contribute to pulmonary carbon dioxide transport. PMID: 19429772

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ammonium transporter (TC 2.A.49) family, Rh subfamily
  • 组织特异性：
    Expressed in the forestomach and the fundus of the stomach. Expressed at the level of villous in duodenum, jejunum, ileum and colon. Expressed in kidney by connecting segments and collecting tubules (at protein level). Expressed in testis by seminiferous
  • 数据库链接：

    KEGG: mmu:56315

    STRING: 10090.ENSMUSP00000032766

    UniGene: Mm.10909