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  4. Recombinant Mouse Antigen peptide transporter 1 (Tap1), partial

Recombinant Mouse Antigen peptide transporter 1 (Tap1), partial

  • 货号：
    CSB-YP023120MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023120MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023120MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023120MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023120MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tap1; Abcb2; Ham-1; Ham1; Antigen peptide transporter 1; APT1; ATP-binding cassette sub-family B member 2; Histocompatibility antigen modifier 1; Peptide transporter TAP1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation.
  • 基因功能参考文献：
    1. TAP-independent self-peptides enhance T cell recognition of immune-escaped tumors. PMID: 26784543
    2. It transport class I antigens to cell surface of CD8 positive lymphocytes which have a vital role in liver inflammation like fructose-induced steatosis. PMID: 23488792
    3. Knocking out Tap1 abolishes NMDAR-dependent LTD in area CA1 of adult mouse hippocampus and is accompanied by memory deficits. MHC class I expression could be an unexpected cause of disrupted synaptic plasticity and cognitive deficits. PMID: 23959708
    4. Apoe(-/-)Tap1(-/-) mice develop atherosclerosis equal to Apoe(-/-) mice, indicating a minor role for CD8(+) T cells and TAP1-dependent antigen presentation in the disease process. PMID: 22479479
    5. Role of metalloproteases in vaccinia virus epitope processing for transporter associated with antigen processing (TAP)-independent human leukocyte antigen (HLA)-B7 class I antigen presentation. PMID: 22298786
    6. Our data suggest that the normal influx of TAP-transported peptides in the ER during routine processing creates an efficient barrier for peptides from alternative processing routes. PMID: 21898382
    7. A complete overview is presented of the applicability of herpesvirus-encoded TAP and tapasin inhibitors in mouse cells of different genetic background. PMID: 21292324
    8. downregulation of expression in PBMC of chronic hepatitis B patients PMID: 20300807
    9. Our results do not support a role for MHC class I (TAP1) in adult neurogenesis, although it may still have a role in the maturation and integration of newborn neurons. PMID: 20147861
    10. a new role for mouse TAP2 in stabilizing TAP1 protein expression is shown using the Jasmine strain; Jasmine has an A to C transversion in exon 5 of TAP2, resulting in a threonine to proline substitution at position 293 of the protein. PMID: 19721454
    11. Allergen-specific CD8+ T cells were recruited to and specifically activated in the lungs of sensitized animals after allergen-aerosol challenge, a process that was dependent on a functional TAP complex PMID: 19815810
    12. MHC conformational changes, revealed at molecular level, may influence the immunogenicity PMID: 11858821
    13. Macrophages cross-presented p33-VLP normally in the absence of TAP. The TAP-dependent pathway of cross-presentation is therefore confined to DC while both macrophages and DC harbor the TAP-independent pathway. PMID: 11870626
    14. A major role for tapasin as a stabilizer of the TAP peptide transporter and consequences for MHC class I expression PMID: 12594855
    15. Tap1 is required for class I major histocompatibility complex (MHC-I) to bind endoplasmic reticulum-derived stabilizing peptides to achieve stability needed for alternate MHC-I processing via peptide exchange in acidic vacuolar processing compartments. PMID: 12794107
    16. Presensitization of TAP1-/- mice with H-2K(b) peptides accelerated the rejection of C57BL/6 (H-2(b)) skin grafts. PMID: 15194347
    17. PSF1 is required for early embryogenesis PMID: 16287864
    18. These data suggest that PSF1 and SLD5 may cooperate in the proliferation of immature cell populations. PMID: 16338220
    19. role for TAP-1 in the induction of IFN-gamma-producing NK (naatural killer) cells and demonstration that NK cell licensing can influence host resistance to infection. PMID: 17923502
    20. regulation of tumor necrosis factor-alpha mRNA nucleocytoplasmic transport by ERK map kinases requires TAP-NxT1 binding and the AU-rich element PMID: 18048358
    21. PSF1 is required for acute proliferation of hematopoietic stem cells in the bone marrow of mice. PMID: 18984863
    22. These data suggest that PSF1 is tightly regulated at the transcriptional level in stem cells. PMID: 19285009
    23. The furin-mediated secretory pathway provides approximately one-third of all surface peptide/histocompatibility class I complexes in wild-type TAP-competent cells. PMID: 19752221

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=The transmembrane segments seem to form a pore in the membrane.
  • 蛋白家族：
    ABC transporter superfamily, ABCB family, MHC peptide exporter (TC 3.A.1.209) subfamily
  • 数据库链接：

    KEGG: mmu:21354

    STRING: 10090.ENSMUSP00000128401

    UniGene: Mm.482076