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  4. Recombinant Mouse Aryl hydrocarbon receptor nuclear translocator (Arnt)

Recombinant Mouse Aryl hydrocarbon receptor nuclear translocator (Arnt)

  • 货号：
    CSB-YP002121MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002121MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002121MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002121MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002121MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Arnt; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-791
  • 氨基酸序列
    AATTANPEM TSDVPSLGPT IASGNPGPGI QGGGAVVQRA IKRRSGLDFD DEVEVNTKFL RCDDDQMCND KERFARSDDE QSSADKERLA RENHSEIERR RRNKMTAYIT ELSDMVPTCS ALARKPDKLT ILRMAVSHMK SLRGTGNTST DGSYKPSFLT DQELKHLILE AADGFLFIVS CETGRVVYVS DSVTPVLNQP QSEWFGSTLY DQVHPDDVDK LREQLSTSEN ALTGRVLDLK TGTVKKEGQQ SSMRMCMGSR RSFICRMRCG TSSVDPVSMN RLSFLRNRCR NGLGSVKEGE PHFVVVHCTG YIKAWPPAGV SLPDDDPEAG QGSKFCLVAI GRLQVTSSPN CTDMSNICQP TEFISRHNIE GIFTFVDHRC VATVGYQPQE LLGKNIVEFC HPEDQQLLRD SFQQVVKLKG QVLSVMFRFR SKTREWLWMR TSSFTFQNPY SDEIEYIICT NTNVKNSSQE PRPTLSNTIP RSQLGPTANL SLEMGTGQLP SRQQQQQHTE LDMVPGRDGL ASYNHSQVSV QPVASAGSEH SKPLEKSEGL FAQDRDPRFP EIYPSITADQ SKGISSSTVP ATQQLFSQGS SFPPNPRPAE NFRNSGLTPP VTIVQPSSSA GQILAQISRH SNPAQGSAPT WTSSSRPGFA AQQVPTQATA KTRSSQFGVN NFQTSSSFSA MSLPGAPTAS SGTAAYPALP NRGSNFPPET GQTTGQFQAR TAEGVGVWPQ WQGQQPHHRS SSSEQHVQQT QAQAPSQPEV FQEMLSMLGD QSNTYNNEEF PDLTMFPPFS E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.
  • 基因功能参考文献：
    1. Results elucidate the dimerization process of AhR with ARNT and propose the structure of the N-terminal region of the AhR:ARNT dimer including the bHLH, PAS-A and PAS-B domains. PMID: 28393157
    2. results provide convincing evidence that reduced hepatic ARNT can contribute to inappropriate hepatic glucose production and post-prandial dyslipidaemia PMID: 29190746
    3. These promoters possess potential signature sequences for common as well as different transcription factors suggesting complex regulation of Arnt gene. PMID: 26707719
    4. The only in vivo defects found in beta-Arnt mice were significant increases in the respiratory exchange ratio and in vivo carbohydrate oxidation, and a decrease in lipid oxidation PMID: 26409461
    5. hepatocyte ARNT is not a requirement for initiation of liver fibrogenesis, but does regulate pro-fibrotic gene expression and macrophage accumulation. PMID: 25812120
    6. HIF-1beta hepatocyte-specific knockout mice had less liver injury and steatosis in response to Gao-binge ethanol treatment. PMID: 25536043
    7. Candidate gene analysis focused on Arnt as an influence on circadian regulation in the 'drinking in the dark' phenotype of alcohol consumption. PMID: 25559016
    8. crystal structures for each of mouse HIF-2alpha-ARNT and HIF-1alpha-ARNT heterodimers in states that include bound small molecules and their hypoxia response element PMID: 26245371
    9. We determined that ARNT is essential for adult and fetal hematopoietic stem cell viability and homeostasis. PMID: 25855602
    10. ARNT is a critical regulator of myocardial fatty acid metabolism and its deletion leads to cardiomyopathy and an increase in triglyceride accumulation through PPARA. PMID: 25329697
    11. This study demonstrates for the first time the requirement of HIF1 for FSH-regulated Vegfa expression in vivo and that HIF1 acts via a single hypoxia response element in the Vegfa promoter to exert its regulatory functions. PMID: 24855100
    12. Outcomes for islet transplants lacking beta-cell ARNT were poor. PMID: 24878748
    13. HIF-1a and its dimerization partner HIF-1b/Arnt occupy the first intron region of the mouse JMJD3 gene. PMID: 24552709
    14. Myeloid ARNT is important for innate immune function and wound healing. PMID: 24990649
    15. results suggest that the HIF-2alpha/ARNT complex regulates hair follicle differentiation via induction of p21(Waf1/Cip1) and possibly p27(Kip1) PMID: 24599965
    16. Islet ARNT increases in normal murine pregnancy and beta-cell ARNT is required for cyclinD2 induction and increased beta-cell proliferation in pregnancy. PMID: 24204824
    17. The ARNT-STAT3 axis is a critical regulator of TCRalphabeta CD8alphaalpha intestinal intraepithelial T-cell development and differentiation. PMID: 23836150
    18. show that exposure of C2C12 myocytes to cyclic hypoxia and reoxygenation rapidly increased levels of IL-10 mRNA, which was blocked by administration of the HIF-1 inhibitor acriflavine or by expression of short hairpin RNA targeting HIF-1alpha or HIF-1beta. PMID: 24101519
    19. Expressions of Dbp and Arnt were dampened in the islets of a diabetic model mouse. PMID: 23567972
    20. Deficiency of HIF-1alpha or HIF-1beta in Tie2-lineage cells resulted in delayed wound closure, reduced vascularization, decreased cutaneous blood flow, impaired bone marrow-derived angiogenic cell (BMDAC) mobilization, and decreased BMDAC homing to burn wounds. PMID: 22028336
    21. In a tumor xenograft model, loss of p53 promotes VEGF production, neovascularization, and tumor progression via accumulation of HIF-1beta protein. PMID: 21324396
    22. Arnt-dependent epidermal-dermal signalling may provide insight into the role of macro- and micro-environmental factors in control of skin vasculature and in pathogenesis of environmentally modulated skin disorders. PMID: 21946855
    23. The Hif1beta/Arnt regulates glucose uptake, mitochondrial gene expression, and vascular permeability to control adipose mass and function. PMID: 21982709
    24. Artificial activation of HIF transcription factors in normoxic photoreceptors results in an increased basal expression of Fgf2 that may contribute to a transient protection of rods against light damage. PMID: 21447692
    25. NF-kB controls the levels of HIF-1a and HIF-1b genes by direct regulation. PMID: 21298084
    26. low O(2) levels promote vascular development and maturation in wild-type embryonic stem cell (ESC) cultures measured by an increase in the numbers of CD31(+) endothelial cells, but refractory in Arnt(-/-) and Vegf(-/-) ESC cultures. PMID: 20135683
    27. ChREBP acts as a novel repressor of the ARNT/HIF-1beta gene and thus may contribute to beta-cell dysfunction induced by glucotoxicity. PMID: 19833882
    28. The coexpression of AHR and ARNT during early tooth development as well as during the formation and mineralization of the dental matrices is suggestive of the AHR-ARNT pathway as a mediator of dental toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin PMID: 12068950
    29. ARNT is involved in transcriptional upregulation of the p27(Kip1) gene under hypoxic conditions. PMID: 14523989
    30. proper ceramide compositions through 4-desaturation regulated by ARNT are crucial for maintaining the epidermal barrier function. PMID: 14597763
    31. formation of stable protein-DNA complexes by DR/Arnt and HIF-1alpha/Arnt heterodimers with their cognate DNA sequences requires Per/Arnt/Sim A domains PMID: 14638687
    32. ARNT is an essential component of aryl hydrocarbon receptor developmental signaling PMID: 14764592
    33. Differential distribution patterns for HIF-1beta and -2beta suggest divergent roles during kidney development for these highly related bHLH-PAS proteins.[IF-1beta] PMID: 15466261
    34. Knockout trophoblast stem cells were able to differentiate into chorionic trophoblasts and syncytiotrophoblasts. PMID: 15987772
    35. The knockdown of ARNT in cultured Min6 cells using siRNA and beta cell-specific knockout of ARNT in mice produces both detectes in glucose-stimulated insulin release and alterations in islet gene expression. PMID: 16096055
    36. for the bHLH.PAS transcription factors Dioxin Receptor and Arnt, the DR PAS A domain has a role in dimerization and affinity for an atypical E-box DNA sequence PMID: 16520375
    37. endothelial Arnt plays a pivotal role in embryonic liver development. PMID: 16941684
    38. The differential binding of AhR by Arnt and Arnt2 can be ascribed to a single His/Pro amino acid difference in the PASB region of Arnt and Arnt2. PMID: 17023418
    39. ARNT controls Cyp2a5 transcription and the ARNT homodimer has a role in mammalian gene regulation PMID: 17466327
    40. Arnt-dependent pathway in gut has an important role in regulation of the metabolism of dietary CYP1A1 inducers and whole-body CYP1A1 expression PMID: 17607366
    41. Cyp1a1 induction by the AHR/ARNT is associated with modification of specific chromatin marks, including hyperacetylation of histone H3K14 and H4K16, trimethylation of histone H3K4, and phosphorylation of H3S10. PMID: 17707923
    42. identified novel hypoxia-regulated genes and confirmed the role of Arnt and HIF-1alpha in regulating their expression PMID: 18959748
    43. Report hypoxia-inducible factor-dependent production of profibrotic mediators by hypoxic hepatocytes. PMID: 19302442
    44. The ARNT protein knockout mice exhibited features of type 2 diabetes with increased hepatic gluconeogenesis, increased lipogenic gene expression, and low serum beta-hydroxybutyrate. PMID: 19416713
    45. Deletion of Arnt in the epidermis completely prevented the induction of skin tumors PMID: 19755658

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:11863

    STRING: 10090.ENSMUSP00000099810

    UniGene: Mm.250265