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  4. Recombinant Mouse Arylamine N-acetyltransferase 1 (Nat1)

Recombinant Mouse Arylamine N-acetyltransferase 1 (Nat1)

  • 中文名称：
    小鼠Nat1重组蛋白
  • 货号：
    CSB-YP015464MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nat1重组蛋白
  • 货号：
    CSB-EP015464MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nat1重组蛋白
  • 货号：
    CSB-EP015464MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nat1重组蛋白
  • 货号：
    CSB-BP015464MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nat1重组蛋白
  • 货号：
    CSB-MP015464MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nat1
  • Uniprot No.：
  • 别名：
    Nat1; Aac1Arylamine N-acetyltransferase 1; EC 2.3.1.5; Arylamide acetylase 1; N-acetyltransferase type 1; NAT-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-290
  • 氨基酸序列
    MDIEAYFERI GYKNSVNKLD LATLTEVLQH QMRAVPFENL NMHCGEAMHL DLQDIFDHIV RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVYITPVSK YSSEMVHLLV QVTISDRKYI VDSAYGGSYQ MWEPLELTSG KDQPQVPAIF LLTEENGTWY LDQIRREQYV PNEEFVNSDL LEKNKYRKIY SFTLEPRVIE DFEYVNSYLQ TSPASVFVST SFCSLQTSEG VHCLVGSTFT SRRFSYKDDV DLVEFKYVNE EEIEDVLKTA FGISLERKFV PKHGELVFTI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Isoniazid, 2-aminofluorene and anisidine are preferred substrates for NAT-1. No activity with p-aminobenzoic acid (PABA) nor SMZ.
  • 基因功能参考文献：
    1. these studies demonstrate that Nat1 deletion promotes reduced mitochondrial activity and is associated with ectopic lipid-induced insulin resistance. These results provide a potential genetic link among mitochondrial dysfunction with increased ectopic lipid deposition, insulin resistance, and type 2 diabetes. PMID: 29237750
    2. Ca(2+)-dependent N-acyltransferase absolutely required Calcium for its activity and the activity was enhanced by phosphatidylserine. PMID: 29447909
    3. Nat1 is involved in the translation of proteins that are required for cell differentiation. PMID: 28003464
    4. This study illustrated that deficiency of NAT1/2 decreases isoniazid (INH) acetylation, but increases the interactions of INH with endobiotics in the liver. PMID: 28888949
    5. our results suggest that Nat1 deficiency results in mitochondrial dysfunction, which may constitute a mechanistic link between this gene and insulin resistance . PMID: 27705799
    6. Differences between murine arylamine N-acetyltransferase type 1 and human arylamine N-acetyltransferase type 2 defined by substrate specificity and inhibitor binding.( PMID: 25432241
    7. In adipocytes, Nat1 silencing lowered insulin-mediated glucose uptake, raised lipolysis, and decreased differentiation. Overexpression did the opposite. Nat1(-) mice had high fasting blood glucose, insulin, and triglycerides and low insulin sensitivity. PMID: 25798622
    8. Transfection assays in mice using hydrodynamics-based procedure and reporter gene assay in a mouse cell line revealed that glucocorticoid-induced NAT gene expression is species dependent PMID: 22644701
    9. NAT1 affects cell growth and morphology PMID: 21347396
    10. identification and functional characterization of novel polymorphisms in mice PMID: 12142728
    11. Biochemical analysis demonstrated that mNAT1 and its evolutionarily conserved co-subunit, mARD1, assemble to form a functional acetyltransferase. PMID: 12888564
    12. Results suggest that peroxynitrite-dependent inactivation of NAT1 and 2 may contribute to muscle dysfunction by impairing the biotransformation activity of this key cellular defense enzyme system. PMID: 16098511

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Arylamine N-acetyltransferase family
  • 数据库链接：

    KEGG: mmu:17960

    STRING: 10090.ENSMUSP00000026677

    UniGene: Mm.14125