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  4. Recombinant Mouse Asporin (Aspn)

Recombinant Mouse Asporin (Aspn)

  • 货号：
    CSB-YP002230MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002230MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002230MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002230MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Aspn
  • Uniprot No.：
  • 别名：
    AspnAsporin; Periodontal ligament-associated protein 1; PLAP-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    33-373
  • 氨基酸序列
    DMEDTDDD DNDDDDNSLF PTKEPVNPFF PFDLFPTCPF GCQCYSRVVH CSDLGLTSVP NNIPFDTRMV DLQNNKIKEI KENDFKGLTS LYALILNNNK LTKIHPKTFL TTKKLRRLYL SHNQLSEIPL NLPKSLAELR IHDNKVKKIQ KDTFKGMNAL HVLEMSANPL ENNGIEPGAF EGVTVFHIRI AEAKLTSIPK GLPPTLLELH LDFNKISTVE LEDLKRYREL QRLGLGNNRI TDIENGTFAN IPRVREIHLE HNKLKKIPSG LQELKYLQII FLHYNSIAKV GVNDFCPTVP KMKKSLYSAI SLFNNPMKYW EIQPATFRCV LGRMSVQLGN VGK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Inhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro.
  • 基因功能参考文献：
    1. Asporin deficiency changes skin glycosaminoglycan composition, and decorin and biglycan content, which may explain the changes in skin mechanical properties. PMID: 28859141
    2. inhibition of TLR2- and TLR4-induced inflammatory responses and binding abilities to TLR2 and TLR4 PMID: 26399972
    3. positively regulates FGF-2 activity through a direct interaction PMID: 26239644
    4. Findings suggest that asporin is one of the important cartilage matrix proteins that binds to the ECM and TGF-beta1 and thereby modulates interactions between TGF-beta and its signaling receptors. PMID: 20052601
    5. PLAP-1/asporin plays a specific role in the periodontal ligament as a negative regulator of cytodifferentiation and mineralization by regulating BMP-2 activity to prevent the periodontal ligament from developing non-physiological mineralization PMID: 17522060
    6. The findings indicate that TGF-beta1 induces ASPN through Smad3 but that this induction is indirect. PMID: 17804408
    7. Leucine-rich repeat motif 5 (LRR5) of PLAP-1/asporin, plays an important role in the PLAP-1/asporin-BMP-2 interaction. PMID: 18407830

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
  • 组织特异性：
    Higher expression in heart, also detected in kidney, stomach, testes, and skin but only weakly in lung, skeletal muscle, small intestine, and thymus. Expressed specifically and predominantly in the periodontal ligament (PDL). During tooth development, str
  • 数据库链接：

    KEGG: mmu:66695

    STRING: 10090.ENSMUSP00000021820

    UniGene: Mm.383216