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  4. Recombinant Mouse Brain-derived neurotrophic factor (Bdnf)

Recombinant Mouse Brain-derived neurotrophic factor (Bdnf)

  • 货号：
    CSB-YP002655MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002655MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002655MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002655MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002655MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BdnfBrain-derived neurotrophic factor; BDNF) [Cleaved into: BDNF precursor form; ProBDNF)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    131-249
  • 氨基酸序列
    HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
  • 基因功能参考文献：
    1. We report here on the incorporation of a BDNF mimetic sequence into a supramolecular peptide amphiphile filamentous nanostructure capable of activating the BDNF receptor TrkB and downstream signaling in primary cortical neurons in vitro. PMID: 30211565
    2. Data (including data from studies in knockout mice) suggest that Bdnf is involved in neuroprotection and regulation of synaptic protein loss in cerebral cortex due to oxidative stress caused by high-fat diet. PMID: 29277119
    3. Total abdominal irradiation exposure impairs cognitive function involving miR-34a-5p/BDNF axis. PMID: 28668331
    4. role in the maintenance of intestinal epithelial barrier function via anti-apoptotic properties PMID: 29527912
    5. upregulation of PAI-1 may be a critical mechanism underlying insufficient neurotrophic support and increased neurodegeneration associated with AD. Thus, targeting BDNF maturation through pharmacological inhibition of PAI-1 might become a potential treatment for AD. PMID: 28132883
    6. These observations suggest that GGA administration is a therapeutic candidate for depressive diseases by increasing hippocampal BDNF levels via HSP105 expression. PMID: 28580422
    7. In a model of traumatic brain injury, transcranial ultrasound stimulation increases brain BDNF levels. PMID: 28939348
    8. Results suggest that brain derived neurotrophic factor (BDNF) activation in the nucleus accumbens (NAc) may overcome memory and plasticity deficits linked to Actl6a protein (BAF53b) mutations. PMID: 27226355
    9. Upregulating BDNF-AS inhibits osteogenesis, possibly through inverse regulation on BDNF and osteogenic signaling pathways PMID: 29247276
    10. miR-322 promotes Tau phosphorylation via negatively controlling BDNF-TrkB receptor activation PMID: 29464486
    11. BDNF preserves intestinal mucosal barrier function and alters gut microbiota in mice. PMID: 29475460
    12. Results demonstrate that heterotrimeric Galphai1 and Galphai3 proteins are essential for TrkB signaling and that disruption of Galphai1 or Galphai3 function could contribute to depressive behaviors. PMID: 29507199
    13. The present study revealed that long-term exercise increased BDNF expression in the motor cortex and facilitated a transfer of motor learning from aerobic exercise to postural coordination. PMID: 29019708
    14. These results suggested that the effects of Valeriana fauriei (VF) extract on a model of fibromyalgia (FM)may be associated with its modulatory effects on the BDNF signaling pathway in the hippocampus and medial prefrontal cortex. In conclusion, the mechanism underlying the protective effects of VF as a therapeutic agent against FM may involve the BDNF signaling pathway PMID: 29115388
    15. findings demonstrate that not only is BDNF actively secreted by the transdifferentiated BDNF-mesenchymal stem cells, but also that it has the capacity to promote neurite sprouting and regeneration PMID: 28694020
    16. Results indicate a modulatory effect of valproic acid on the Bbrain-derived neurotrophic factor levels in the ventral striatum. Study brings initial insights into the involvement of neurotrophic mechanisms in the ventral striatum in ethanol-induced addictive-like behavior. PMID: 28889008
    17. significant increase of BDNF levels in the hippocampus and prefrontal cortex was observed in ethanol-treated mice receiving ginseng extract G115. PMID: 28837087
    18. This findings consistently demonstrate that long-lasting nociceptive input activates VTA neurons and, via increased release of BDNF. PMID: 28390647
    19. increased BDNF-TrkB signaling and synaptogenesis in the nucleus accumbens by deletion of alpha7 nAChR plays a key role in depression PMID: 27821848
    20. Findings suggest that carriers of the BDNF-Met allele may exhibit greater vulnerability to anxiety disorders and anorexia nervosa, contributed by increased excitability of pyramidal neurons, due to the combination of decreased GABAergic innervation of distal dendrites and BDNF-mediated suppression of perisomatic inhibitory inputs. PMID: 27578497
    21. the ZnT3 null state removed synaptic zinc, it rather increased free zinc in the cytosol of brain cells, which appeared to increase MMP-9 activity and BDNF levels. The present results suggest that zinc dyshomeostasis during the critical period of brain development may be a possible contributing mechanism for ASD. PMID: 27352957
    22. the BDNFVal66Met polymorphism contribute to the individual propensity for arterial thrombosis related to acute myocardial infarction PMID: 26705390
    23. There was a significant decrease in the BDNF protein expression along with an increase in the mRNA expression of CRH, NR3C1, CART, and NPY in intact females PMID: 28527954
    24. Daily OMR testing during the critical period leads to general visual function improvements accompanied by increased DA and BDNF in the retina, with this process being requisitely mediated by TrkB activation PMID: 29408880
    25. The changes in the expression patterns of proBDNF, BDNF, and their receptors under the influence of chronic alcohol consumption in the depressive ASC strain and nondepressive CBA strain mice are different. PMID: 28900083
    26. These results suggest that BDNF is implicated in MDMA-induced dependence and psychosis by activating the midbrain serotonergic and dopaminergic neurons. PMID: 28472632
    27. newborn neurons improve social recognition persistence through a BDNF-independent mechanism. PMID: 27165290
    28. Study shows long-term physical exercise in mice led to a significant increase in the synthesis and release of brain derived neurotrophic factor along with an alteration in the morphology of astrocytes in the dentate gyrus. PMID: 27686571
    29. Mice exposed to aggressive confrontations exhibited a similar pattern of species-typical aggressive and non-aggressive behaviors on the first and the last session. Repeated aggressive confrontations promoted an increase in plasma corticosterone. Repeated sessions of social instigation or aggressive confrontation did not alter BDNF concentrations at the prefrontal cortex and hippocampus. PMID: 28614436
    30. High-intensity acute exercise increased total Bdnf mRNA in hippocampus of sedentary mice. PMID: 28556463
    31. Thus, the expression of BDNF in the hippocampal CA1 region has the potential to improve fear and object location memory in wild type mouse strains when the region and expression levels of BDNF are well controlled. PMID: 27394686
    32. Study demonstrates the importance of 14,15-EET-mediated production of astrocyte-derived brain derived neurotrophic factor for enhancing viability of astrocytes and protecting neurons from the ischaemic injury and provides insights into the mechanism by which 14,15-EET is involved in neuroprotection. PMID: 26526810
    33. Orthopedic surgery modulates the expression of neuropeptide Y and BDNF at the spinal and hippocampal levels. PMID: 27791037
    34. treating BACHD cortical neurons with ApiCCT1 prevented BACHD striatal neuronal atrophy by enhancing release of BDNF that subsequently acts through tyrosine receptor kinase B (TrkB) receptor on striatal neurons. Our findings are evidence that TRiC reagent-mediated reductions in mHTT enhanced BDNF delivery to restore the trophic status of BACHD striatal neurons. PMID: 27601642
    35. BDNF in the lower parts of the auditory system drives auditory fidelity along the entire ascending pathway up to the cortex by increasing inhibitory strength in behaviorally relevant frequency regions. PMID: 26476841
    36. Results highlight the biological significance of alternative brain-derived neurotrophic factor transcripts and provide evidence that individual isoforms serve distinct molecular and behavioral functions. PMID: 26585288
    37. A critical period of susceptibility to cigarette smoke exposure exists in the prenatal and early postnatal period, which results a downregulation in brain-derived neurotrophic factor/tyrosine kinase receptor B signaling in the hippocampus and enhances depression-like behaviors later in life. PMID: 26503133
    38. Deletion of Bdnf in DRG neurons leads to a temporary dysregulation of miR-1. PMID: 27346077
    39. Excitatory synaptic activation-dependent up-regulation of XBP1 directly facilitated transcriptional activation of BDNF. BDNF in turn drove its own expression via IRE1-XBP1 pathway in a protein kinase A-dependent manner. Exogenous treatment with BDNF promoteSynaptic activity- and BDNF-dependent distinct activation of dendritic IRE1-XBP1 cascade drives BDNF expression in cell soma and may be involved in dendritic extension. PMID: 28921568
    40. We hypothesize that BDNF in the developing brain regulates fear circuit plasticity during a sensitive period in early adolescence, and alterations in BDNF expression (genetic or environmental) have a persistent impact on fear behavior and fear-related disorders. PMID: 27699937
    41. High BDNF expression is associated with neuropathic pain. PMID: 29054488
    42. The s show that BDNF acts cell autonomous and autocrine, as wildtype neurons are not capable of rescuing growth deficits in neighboring MeCP2 deficient neurons in vitro and in vivo. PMID: 27782879
    43. BDNF acts retrogradely on TrkB in climbing fibers , and facilitates elimination of climbing fibers synapses from Purkinje cells somata during the third postnatal week. PMID: 28775326
    44. EGCG significantly ameliorated insulin resistance and cognitive disorder by up-regulating the insulin receptor substrate-1 (IRS-1)/AKT and ERK/cAMP response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathways. PMID: 28739640
    45. Results suggest that activity-dependent BDNF signaling is critical for regulating oscillatory activity, which may contribute to altered behavior. PMID: 27552586
    46. the transcriptional induction of Bdnf by a mnemonic stimulus is impaired in aged hippocampal neurons. PMID: 27626660
    47. BDNF deficiency results in depression-like behavior, regardless of age and gender. PMID: 27648918
    48. Study reports that an endogenous molecule released after exercise is capable of inducing key promoters of the Mus musculus Bdnf gene. The metabolite beta-hydroxybutyrate, which increases after prolonged exercise, induces the activities of Bdnf promoters, particularly promoter I, which is activity-dependent. PMID: 27253067
    49. Results show that (1) large-soma retinal ganglion cells are particularly susceptible to optic nerve injury; and (2) overexpression of BDNF prolongs the survival of retinal ganglion cells, including large-soma retinal ganglion cells. PMID: 28101532
    50. GR downregulates Bdnf expression through direct binding to Bdnf regulatory sequences. PMID: 28403881

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  • 亚细胞定位：
    Secreted.; [BDNF precursor form]: Secreted.
  • 蛋白家族：
    NGF-beta family
  • 组织特异性：
    Expressed in the dorsal root ganglion and the spinal cord (at protein level). Detected in brain, especially in brain cortex, hippocampus, midbrain and cerebellum.
  • 数据库链接：

    KEGG: mmu:12064

    STRING: 10090.ENSMUSP00000057989

    UniGene: Mm.1442