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  4. Recombinant Mouse C->U-editing enzyme APOBEC-1 (Apobec1)

Recombinant Mouse C->U-editing enzyme APOBEC-1 (Apobec1)

  • 中文名称：
    Recombinant Mouse C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 货号：
    CSB-YP001919MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 货号：
    CSB-EP001919MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 货号：
    CSB-EP001919MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 货号：
    CSB-BP001919MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 货号：
    CSB-MP001919MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Apobec1
  • Uniprot No.：
  • 别名：
    Apobec1C->U-editing enzyme APOBEC-1; EC 3.5.4.36; Apolipoprotein B mRNA-editing enzyme 1; mRNA(cytosine(6666)) deaminase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-229
  • 氨基酸序列
    MSSETGPVAV DPTLRRRIEP HEFEVFFDPR ELRKETCLLY EINWGGRHSV WRHTSQNTSN HVEVNFLEKF TTERYFRPNT RCSITWFLSW SPCGECSRAI TEFLSRHPYV TLFIYIARLY HHTDQRNRQG LRDLISSGVT IQIMTEQEYC YCWRNFVNYP PSNEAYWPRY PHLWVKLYVL ELYCIILGLP PCLKILRRKQ PQLTFFTITL QTCHYQRIPP HLLWATGLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.
  • 基因功能参考文献：
    1. APOBEC1-mediated RNA editing occurs within brain microglia cells and is key to maintaining their resting status. Apobec1-/- mice display age-related signs of CNS neurodegeneration. PMID: 29167375
    2. Here, we show that partial loss of either APOBEC1 complementation factor (A1CF), the RNA-binding cofactor of APOBEC1 in RNA editing, or Argonaute 2 (AGO2), a key factor in the biogenesis of certain noncoding RNAs, modulates risk for TGCTs and testicular abnormalities in both parent-of-origin and conventional genetic manners. PMID: 27582469
    3. substantial rearrangement/duplication of transgene elements is present, and transgene integration was accompanied by the deletion of a 19,500 bp fragment of genomic DNA that contains the promoter, exon 1 and part of intron 1 of the APOBEC1 complementation factor (A1cf) gene, as well as several elements that are predicted to regulate chromosomal architecture PMID: 27165291
    4. The observations demonstrate that A1CF does not act as the APOBEC1 complementation factor in vivo under normal physiological conditions and suggest new roles for A1CF, specifically within the male adult kidney. PMID: 28069890
    5. Apobec-1-dependent C-to-U RNA editing exerts broad functional effects in a tissue-specific manner. PMID: 24946870
    6. RBM47 and APOBEC1 constitute the basic machinery for C to U RNA editing. PMID: 24916387
    7. In contrast to in vitro results, APOBEC1 neither inhibited nor significantly drove the molecular evolution of Friend retrovirus in wild type or APOBEC1 knockout mice. PMID: 25303118
    8. Individual genetic variability at the Apobec1 locus results in differential rates of C-to-U(T) editing in murine macrophages; with mouse strains expressing mostly a truncated alternative transcript isoform of Apobec1 exhibiting lower rates of editing. PMID: 24249727
    9. The transgenic rescue of intestinal apobec-1 expression restores C-to-U RNA editing of apoB mRNA in vivo, including the canonical site at position 6666 and also at approximately 20 other newly identified downstream sites present in WT mice. PMID: 22993231
    10. Results suggest that apo B mRNA editing protein (Apobec1 cytidine deaminase) plays a central role in controlling testicular germ cell tumors susceptibility in both a conventional and a transgenerational manner. PMID: 22923694
    11. The transcriptomics approach to RNA editing presented in this study dramatically expands the list of APOBEC1 mRNA editing targets and reveals a novel cellular mechanism for the modification of transcript 3' UTRs. PMID: 21258325
    12. LDL receptor and the apolipoprotein B mRNA editing enzyme Apobec1 are regulated via calcium signaling in mechanistic response to genetic, mechanical, and environmental insults that trigger an imbalance of intracellular calcium homeostasis PMID: 20667539
    13. studies establish the existence of preferential degradation of intestinal apolipoprotein B-100 and subtle defects in triglyceride secretion in apolipoprotein B editing complex 1-/- mice PMID: 12816761
    14. apobec-1 complementation factor has a role in regulating nucleocytoplasmic import and shuttling PMID: 12896982
    15. apobec-1-mediated apoB mRNA editing is regulated by BAG-4 PMID: 14559896
    16. RNA editing of this protein is unable to induce somatic hypermutation in mammalian cells. PMID: 14559972
    17. Lipopolysaccharide increases intestinal stem cell survival through apobec-1-mediated regulation of cyclooxygenase-2 messenger RNA stability. PMID: 15480992
    18. Deletion of apobec-1, by modulating expression of AU-rich RNA targets, provides an important mechanism for attenuating a dominant genetic restriction point in intestinal adenoma formation. PMID: 17875695
    19. Murine APOBEC1 is a hypermutator of both RNA and single-stranded DNA in vivo, which could exert occasional side effects upon overexpression. PMID: 18983852
    20. the AU-rich RNA binding-protein Apobec-1 mediates post-transcriptional regulation of Cyp7a1 expression and influences susceptibility to diet-induced gallstone formation PMID: 19386592

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Cytidine and deoxycytidylate deaminase family
  • 组织特异性：
    Expressed in the spleen. Expressed at lower level in the kidney, testis, lung, brain and liver.
  • 数据库链接：

    KEGG: mmu:11810

    STRING: 10090.ENSMUSP00000108204

    UniGene: Mm.3333