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  4. Recombinant Mouse CASP8 and FADD-like apoptosis regulator (Cflar)

Recombinant Mouse CASP8 and FADD-like apoptosis regulator (Cflar)

  • 货号：
    CSB-YP005282MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005282MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005282MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005282MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005282MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cflar; CashCASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; Usurpin) [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-380
  • 氨基酸序列
    MAQSPVSAEV IHQVEECLDE DEKEMMLFLC RDVTENLAAP NVRDLLDSLS ERGQLSFATL AELLYRVRRF DLLKRILKTD KATVEDHLRR NPHLVSDYRV LLMEIGESLD QNDVSSLVFL TRDYTGRGKI AKDKSFLDLV IELEKLNLIA SDQLNLLEKC LKNIHRIDLN TKIQKYTQSS QGARSNMNTL QASLPKLSIK PSVLYLKLQN GRSKEPRFVE YRDSQRTLVK TSIQESGAFL PPHIREETYR MQSKPLGICL IIDCIGNDTK YLQETFTSLG YHIQLFLFPK SHDITQIVRR YASMAQHQDY DSFACVLVSL GGSQSMMGRD QVHSGFSLDH VKNMFTGDTC PSLRGKPKLF FIQNYESLGS QLEDSSLEVD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • 基因功能参考文献：
    1. Deletion of c-FLIP from CD11b(hi) Macrophages Prevents Development of Bleomycin-induced Lung Fibrosis. PMID: 28850249
    2. c-FLIP modulation of AKT activity is crucial in controlling PERK signalling and sensitivity to endoplasmic reticulum stress. PMID: 27267061
    3. Regulatory T cells have a high apoptosis rate ex vivo correlating with low c-FLIP expression. PMID: 28052242
    4. In conclusion, our data indicated that miR-150 potentially contributes to the hepatic steatosis and insulin resistance in NAFLD. miR-150/CFLAR pathway may be a new therapeutic strategy against NAFLD. PMID: 29107687
    5. Findings establish CFLAR as a key suppressor of steatohepatitis and indicate that the development of CFLAR-peptide-mimicking drugs and the screening of small-molecular inhibitors that specifically block ASK1 dimerization are new and feasible approaches for NASH treatment. PMID: 28218919
    6. knockdown of cFLIPL and induced expression of FADD rapidly accumulate intracellular ROS accompanied by JNK1 activation to substantiate apoptosis. PMID: 27619661
    7. CASP8 is present exclusively as its cleaved p43 product, bound to cFLIPL. PMID: 26798068
    8. The generation of mouse line with Flip deficiency in cells that express cre under the CD11c promoter is reported. PMID: 25963626
    9. c-FLIPL deficiency induces the caspase-mediated processing of RTN4, thus affecting endoplasmic reticulum (ER) shape and coupling to mitochondria. Thus, it was concluded that c-FLIPL is a novel regulator of ER morphology and ER-mitochondria crosstalk. PMID: 25501600
    10. Acute organ failure following the loss of anti-apoptotic cellular FLICE-inhibitory protein involves activation of innate immune receptors. PMID: 25342470
    11. The results reveal a novel inhibitory role of c-FLIP in myeloid cell activation and demonstrate the unexpected anti-inflammatory activity of c-FLIP. PMID: 26238491
    12. Upon starvation, c-Flip affects lipid accumulation, ER stress and autophagy, thereby pointing to an important role of c-Flip in the adaptive response and ER stress response programs under both normal and pathological conditions. PMID: 25746012
    13. Flip preserves cardiac functions and inhibits cardiac hypertrophy partially by blocking ASK1/P38 signaling. PMID: 25087120
    14. These data suggest that c-FLIP is a negative regulator of intrinsic apoptosis pathway in T lymphocytes. PMID: 25725104
    15. c-FLIPR is an important modulator of apoptosis and enforced expression leads to autoimmunity. PMID: 24722293
    16. controls the development of monocyte-derived myeloid suppressor cells; constitutive expression distinguishes tumor-resident cells PMID: 25500368
    17. cFLIP protein expression from one allele of cFLIP is sufficient to protect epidermal keratinocytes from cell death and a macroscopic phenotype upon acute ablation of the cFLIP locus. PMID: 24209745
    18. cFLIP regulates cellular injury from apoptosis signaling pathways. PMID: 23828575
    19. Cellular FLICE-like inhibitory protein secures intestinal epithelial cell survival and immune homeostasis by regulating caspase-8. PMID: 24036366
    20. A pathway involving HDAC5, cFLIP and caspases regulates expression of the splicing regulator polypyrimidine tract binding protein in the heart. PMID: 23424201
    21. Raji isoform supports immune response against bacterial infection PMID: 23505065
    22. c-FLIP protein has an important role in the cisplatin resistance of human bladder cancer cells. PMID: 23313194
    23. c-FLIPL-deficient T cells underwent RIP-1-dependent necroptosis upon TCR stimulation. PMID: 23175183
    24. show that fibrotic lung myofibroblasts divert Fas signaling from apoptosis to proliferation and that this requires signaling by TNF receptor-associated factor (TRAF) and NF-kappaB. c-FLIP down-regulation reverses the effect of Fas activation PMID: 22582174
    25. Deletion of cFLIP in hepatocytes leads to increased fibrosis and accelerated fibrosis progression. This is accompanied by increased injury involving the activation of caspases and JNK2. PMID: 22700824
    26. protects against the development of post-infarction cardiac remodelling PMID: 22202974
    27. c-FLIP is a central regulator of cell death in hepatocytes, involving increased activation of caspases and the MAPK JNK. PMID: 21703207
    28. This finding indicates a novel additional mechanism through which c-Flip might possibly control tissue remodeling. PMID: 21364645
    29. Flip expression is regulated by FoxO3a PMID: 20739833
    30. FLIP is necessary for macrophage differentiation and the homeostatic regulation of granulopoiesis. PMID: 20724542
    31. The influence of FasL on the cellular regulation of c-FLIP protein levels appears to be a unique mechanism for modulating germ cell apoptosis after toxicant-induced Sertoli cell injury. PMID: 20100735
    32. we uncover a new role for FLIP-L as an unexpected critical player in neurotrophin-induced mitogen-activated protein kinase/ERK- and NF-B-mediated control of neurite growth in developing neurons PMID: 20427667
    33. The data show that c-FLIP(L) forced expression in haploid male germ cells has detrimental effects on spermatogenesis and sperm quality and reveal a possible mechanism underlying the onset of testicular atrophy. PMID: 19285665
    34. TRP53 protein influences the sensitivity of GC-2 cells to undergo FAS-mediated apoptosis by modulating the expression of FAS on their cell membranes and subsequently influencing the degradation of the antiapoptotic protein CFLAR (L) PMID: 16306425
    35. FLIP is found to be selectively overexpressed in the lymph nodes of IL-2-deficient mice, but no overexpression is found in spleen cells or thymocytes. PMID: 12244156
    36. The consequences of FasL overexpression depend on the subcellular compartment and species in which FasL enforced expression is targeted and this is at least partially related to FLIP levels PMID: 12477972
    37. FLIP is downregulated in an apoptosis pathway involving FOXO3a and Akt PMID: 14551207
    38. Data show that inhibition of extracellular signal-regulated kinase (ERK1/2) sensitizes wild-type but not keratin 8 (K8)-null mouse hepatocytes to apoptosis, and is associated with a reduction in c-Flip protein, part of a K8/K18 complex PMID: 15282307
    39. Constitutive overexpression of c-FLIP (long form) in T cells is sufficient to drive Th2 polarization of effector T cell responses and indicates that it might function as a key regulator of T helper cell differentiation. PMID: 15557152
    40. differential spatial and temporal regulation of cFLIP-alpha and cFLIP-delta expression that may influence the magnitude of cell death PMID: 15815586
    41. c-FLIP knock-down with a small interfering RNA significantly restores Fas-mediated apoptosis in infected cells PMID: 15917295
    42. c-Flip expression in fetal testes and its role in protecting gonocytes from Fas-dependent apoptosis are reported. PMID: 16263940
    43. transgenic mice with thyrocyte-specific expression of FLIP have normal thyroid development PMID: 16487007
    44. analyses showed Fas expression but no significant caspase 8/10 activity when c-Flip(L) was highly expressed, suggesting that c-Flip may control the survival rate of undifferentiated spermatogonia PMID: 17056040
    45. Selective inhibition of IkappaB kinase led to decreased c-FLIP expression and allowed all MCL samples to undergo TRAIL-mediated apoptosis PMID: 17237443
    46. Intrinsic resistance to TNF-alpha-induced hepatocyte apoptosis in ICR mice correlates with expression of Cflar. PMID: 17372587
    47. Knocking down FLIP expression abolishes the ability of Met to inhibit Fas-triggered hepatocyte death, demonstrating the functional requirement of FLIP in HGF anti-apoptotic signals. PMID: 17464994
    48. FLIP(L) is essential for tumor necrosis factor (TNF)/tumor necrosis factor receptor I (TNFRI)-mediated neuroprotection after glucose deficiency. PMID: 17581950
    49. Results indicate that transgenic FLIP expressed on thyroid epithelial cells in CBA/J mice promotes G-EAT resolution, but induction of G-EAT is inhibited unless CD8+ T cells are transiently depleted. PMID: 17823262
    50. c-FLIP(S) functions to reduce the expansion of T cells during an immune response PMID: 18081036

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  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Highly expressed in heart.
  • 数据库链接：

    KEGG: mmu:12633

    STRING: 10090.ENSMUSP00000065107

    UniGene: Mm.336848