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  4. Recombinant Mouse CCR4-NOT transcription complex subunit 7 (Cnot7)

Recombinant Mouse CCR4-NOT transcription complex subunit 7 (Cnot7)

  • 中文名称：
    Recombinant Mouse CCR4-NOT transcription complex subunit 7(Cnot7),Yeast
  • 货号：
    CSB-YP730662MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse CCR4-NOT transcription complex subunit 7(Cnot7),Yeast
  • 货号：
    CSB-EP730662MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse CCR4-NOT transcription complex subunit 7(Cnot7),Yeast
  • 货号：
    CSB-EP730662MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse CCR4-NOT transcription complex subunit 7(Cnot7),Yeast
  • 货号：
    CSB-BP730662MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse CCR4-NOT transcription complex subunit 7(Cnot7),Yeast
  • 货号：
    CSB-MP730662MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cnot7
  • Uniprot No.：
  • 别名：
    Cnot7; Caf1CCR4-NOT transcription complex subunit 7; EC 3.1.13.4; CCR4-associated factor 1; CAF-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-285
  • 氨基酸序列
    MPAATVDHSQ RICEVWACNL DEEMKKIRQV IRKYNYVAMD TEFPGVVARP IGEFRSNADY QYQLLRCNVD LLKIIQLGLT FMNEQGEYPP GTSTWQFNFK FNLTEDMYAQ DSIELLTTSG IQFKKHEEEG IETQYFAELL MTSGVVLCEG VKWLSFHSGY DFGYLIKILT NSNLPEEELD FFEILRLFFP VIYDVKYLMK SCKNLKGGLQ EVAEQLELER IGPQHQAGSD SLLTGMAFFK MREMFFEDHI DDAKYCGHLY GLGSGSSYVQ NGTGNAYEEE ASKQS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for miRNA-mediated mRNA deadenylation. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
  • 基因功能参考文献：
    1. This study identifies CNOT7 as an important regulator of RNA transport and translation in dendrites, as well as higher cognitive function. PMID: 28723570
    2. study reports that a complex of BTG4 and CAF1 safeguards metaphase II arrest in mammalian eggs by deadenylating maternal mRNAs PMID: 27605379
    3. Cnot7, a key component of deadenylation machinery, are also dormant maternal mRNAs that are recruited during oocyte maturation PMID: 26134871
    4. Collectively, our data support a model of CNOT7, TOB1, CNOT1, and RNA-binding proteins collectively exerting post-transcriptional control on a metastasis suppressive transcriptional program to drive tumor cell metastasis PMID: 26807845
    5. mCAF1-deficient mouse phenotype could be linked to a defect(s) in germ cells and/or to inadequate Sertoli cell function, leading to seminiferous tubule disorganization and finally to a total disappearance of germ cells PMID: 15199137
    6. Gene deletion of Cnot7 caused abnormal sperm morphology in Sertoli cells from knockout mice. PMID: 15700538
    7. Cnot7 is expressed in osteoblasts and is associated with mineralization. PMID: 16639713
    8. Cnot7 is an endogenous suppressor of bone mass in adult mice and inhibits bone mass phenotype actions PMID: 17451368
    9. Show that a let-7 miRNA-loaded RNA-induced silencing complex interacts with the poly(A)-binding protein and the CAF1 and CCR4 deadenylases for miRNA mediated deadenylation. PMID: 19716330

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  • 亚细胞定位：
    Nucleus. Cytoplasm, P-body. Note=NANOS2 promotes its localization to P-body.
  • 蛋白家族：
    CAF1 family
  • 数据库链接：

    KEGG: mmu:18983

    STRING: 10090.ENSMUSP00000034012

    UniGene: Mm.272551