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  4. Recombinant Mouse CREB-regulated transcription coactivator 2 (Crtc2)

Recombinant Mouse CREB-regulated transcription coactivator 2 (Crtc2)

  • 中文名称：
    Recombinant Mouse CREB-regulated transcription coactivator 2(Crtc2),Yeast
  • 货号：
    CSB-YP663643MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse CREB-regulated transcription coactivator 2(Crtc2),Yeast
  • 货号：
    CSB-EP663643MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse CREB-regulated transcription coactivator 2(Crtc2),Yeast
  • 货号：
    CSB-EP663643MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse CREB-regulated transcription coactivator 2(Crtc2),Yeast
  • 货号：
    CSB-BP663643MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse CREB-regulated transcription coactivator 2(Crtc2),Yeast
  • 货号：
    CSB-MP663643MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Crtc2; Torc2CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2; TORC-2; Transducer of CREB protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-692
  • 氨基酸序列
    ATSGANGPG SATASASNPR KFSEKIALQK QRQAEETAAF EEVMMDIGST RLQAQKLRLA YTRSSHYGGS LPNVNQIGCG LAEFQSPLHS PLDSSRSTRH HGLVERVQRD ARRMVSPLRR YPRHIDSSPY SPAYLSPPPE SGWRRMMPWG NLPAEKGQLF RLPSALNRTS SDSALHTSVM NPNPQDTYPG PTPPSVLPSR RGGGFLDGEM DAKVPAIEEN VVDDKHLLKP WDAKKLSSSS SRPRSCEVPG INIFPSPDQP ANVPVLPPAM NTGGSLPDLT NLHFPPPLPT PLDPEETVYP SLSGGNSTTN LTHTMTHLGI SGGLGLGPSY DVPGLHSPLS HPSLQSSLSN PNLQASLSSP QPQLQGSHSH PSLPASSLAH HALPTTSLGH PSLSAPALSS SSSSSSTSSP VLSAPPYPAS TPGASPRHRR VPLSPLSLPA GPADARRSQQ QLPKQFSPTM SPTLSSITQG VPLDTSKLPT DQRLPPYPYS PPSLVIPTHP PTPKSLQQLP SQACLVQPSG GQPPGRQPHY GALYPPGSSG HGQQPYHRPI NDFSLGNLEQ FNMESPSTSL VLDPPAFSEG PGFLGSEGSM SGPQDPHVLN HQNLTHCSRH GSGPNIILTG DSSPGFSKEI AAALAGVPGF EVSASGLELG LGLEDELRME PLGLEGLTML SDPCALLPDP AVEDSFRSDR LQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated. Acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells.
  • 基因功能参考文献：
    1. CRTC2 regulates the expression of Smurf1 in osteoblast differentiation. PMID: 30449452
    2. data show that the CREB/CRTC2-dependent transcriptional pathway is critical for regulating glucose homeostasis by controlling production of GLP-1 from the L cells at the level of transcription, maturation, and exocytosis. PMID: 29118086
    3. Study suggests that Creb/Crtc2 negatively regulates the Sirt1/Pparalpha/Fgf21 axis via the induction of miR-34a under diet-induced obesity and insulin-resistant conditions. PMID: 29192248
    4. Thyroid stimulating hormone activates CRTC2 via the TSHR/cAMP/PKA pathway. PMID: 28212844
    5. CRTC2 play a role in hepatic cholesterol synthesis through SREBP-2. PMID: 28395113
    6. Catecholaminergic deletion of Rictor increases water, sucrose, and morphine intake but not preference in a two-bottle choice assay in stress-naive mice, and these effects are maintained after stress. Ventral Tegmental Area-specific knockout of Rictor increases water and sucrose intake after physical CSDS, but does not alter consummatory behavior in the absence of stress. PMID: 28167137
    7. the protein expression of adipose tissue CRTC 2 was reduced in mice with a combined application of change to general diet and exercise. In addition, while the protein expressions of lipase ATGL and HSL were reduced in the mice fed with the high fat diet continually after obesity was induced PMID: 27596982
    8. CRTC2 promotes Th17 cell differentiation via stimulation of IL-17A and IL-17F expression by binding to CREB over both promoters. CRTC2-mutant mice have decreased Th17 cells, and they are protected from experimental autoimmune encephalitis. PMID: 26031354
    9. chronic increases in CRTC2 activity in the liver are indeed sufficient to promote hepatic insulin resistance and to disrupt glucose homeostasis PMID: 26342077
    10. cAMP, SIK and CRTC mediate StAR expression through activation of individual StAR gene loci. PMID: 25662274
    11. RICTOR/mTORC2 is important for interactions between vasculature, adipocytes, and brain to tune physiological outcomes, such as blood pressure and locomotor activity. PMID: 26101345
    12. CREB regulated transcription coactivator 2 (CRTC2) functions as a mediator of mTOR signalling to modulate COPII-dependent SREBP1 processing PMID: 26147081
    13. Translational activation of CREB is caused by elevated phospho-elF2alpha. PMID: 24889041
    14. CRTC2 mRNA levels down-regulation improves glucose control and other markers of metabolic function in experimental type 2 diabetes mellitus. PMID: 24380393
    15. PRMT6 is involved in the regulation of hepatic glucose metabolism in a CRTC2-dependent manner. PMID: 24570487
    16. Crtc2-overexpressing mice have increased myofiber cross-sectional area, greater intramuscular triglycerides and glycogen content. PMID: 24674967
    17. Data suggest that promoting Crtc2-Creb activity is required for beta-cell function and proliferation and promoting this pathway could ameliorate symptoms of new onset diabetes after transplantation. PMID: 23677932
    18. CRTC2 inhibition resulted in reduced expression of several glucagon-induced pyridoxal 5'-phosphate-dependent enzymes that convert amino acids to gluconeogenic intermediates PMID: 23595987
    19. Differential regulation of corticotrophin-releasing hormone (CRH) relies upon the cAMP response-element binding protein coactivator CRTC2, which serves as a switch for BDNF and glucocorticoids to direct the expression of CRH. PMID: 22232675
    20. the association of Pin1 with CRTC2 to decrease the nuclear CBP.CRTC.CREB complex. PMID: 20675384
    21. Targeted disruption of the CREB coactivator Crtc2 increases insulin sensitivity. PMID: 20133702
    22. hormonal and energy-sensing pathways converge on the coactivator TORC2 (transducer of regulated CREB activity 2) to modulate glucose output PMID: 16148943
    23. insulin inhibits gluconeogenic gene expression during re-feeding by promoting the phosphorylation and ubiquitin-dependent degradation of TORC2 PMID: 17805301
    24. findings show that O-glycosyl transferase triggered hepatic gluconeogenesis through the O-glycosylation of CRTC2; CRTC2 was O-glycosylated at sites that normally sequester CRTC2 in the cytoplasm through a phosphorylation-dependent mechanism PMID: 18323454
    25. data propose that dysregulation of TORC2 would further exaggerate insulin resistance and promote type 2 diabetes in a LIPIN1-dependent manner PMID: 19254569
    26. SIK2-TORC2 cascade may be important for the regulation of PGC-1alpha and UCP-1 gene expression in insulin signaling in brown adipose tissue PMID: 19351809
    27. CRTC2 functions as a dual sensor for ER stress and fasting signals PMID: 19543265
    28. CREB-regulated transcription coactivator 2 deficient mice did not display fasting hypoglycemia despite impairments in the transcription of several metabolic genes PMID: 19583954
    29. data identify CRTC2 as a new hypothalamic AMP-activated protein kinase target and highlight a role for CRTC2 in the mechanisms linking hypothalamic glucose sensing with CRE gene regulation. PMID: 19713961

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TORC family
  • 组织特异性：
    Expressed in the suprachiasmatic nucleus (SCN) of the brain.
  • 数据库链接：

    KEGG: mmu:74343

    STRING: 10090.ENSMUSP00000029545

    UniGene: Mm.35627