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  4. Recombinant Mouse Calcium/calmodulin-dependent protein kinase kinase 2 (Camkk2)

Recombinant Mouse Calcium/calmodulin-dependent protein kinase kinase 2 (Camkk2)

  • 货号：
    CSB-YP805375MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP805375MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP805375MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP805375MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP805375MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Camkk2; Kiaa0787Calcium/calmodulin-dependent protein kinase kinase 2; CaM-KK 2; CaM-kinase kinase 2; CaMKK 2; EC 2.7.11.17; Calcium/calmodulin-dependent protein kinase kinase beta; CaM-KK beta; CaM-kinase kinase beta; CaMKK beta
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-588
  • 氨基酸序列
    SSCVSSQPT SDRVAPQDEL GSGGGSREGQ KPCEALRGLS SLSIHLGMES FIVVTECEPG RGVDLNLARD QPPEADGQEL PLEASDPESR SPLSGRKMSL QEPSQGGPAS SSNSLDMNGR CICPSLSYSP ASSPQSSPRM PRRPTVESHH VSITGLQDCV QLNQYTLKDE IGKGSYGVVK LAYNENDNTY YAMKVLSKKK LIRQAGFPRR PPPRGARPAP GGCIQPRGPI EQVYQEIAIL KKLDHPNVVK LVEVLDDPNE DHLYMVFELV NQGPVMEVPT LKPLSEDQAR FYFQDLIKGI EYLHYQKIIH RDIKPSNLLV GEDGHIKIAD FGVSNEFKGS DALLSNTVGT PAFMAPESLS ETRKIFSGKA LDVWAMGVTL YCFVFGQCPF MDERIMCLHS KIKSQALEFP DQPDIAEDLK DLITRMLDKN PESRIVVPEI KLHPWVTRHG AEPLPSEDEN CTLVEVTEEE VENSVKHIPS LATVILVKTM IRKRSFGNPF EGSRREERSL SAPGNLLTKK PTREWEPLSE PKEARQRRQP PGPRAGPCGG GGSALVKGGP CVESWGAPAP GSPPRMPPLQ PEEVMEPE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK4 and CAMK1D. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals. May play a role in neurite growth. Isoform 2 may promote neurite elongation, while isoform 1 may promoter neurite branching. May be involved in hippocampal activation of CREB1.
  • 基因功能参考文献：
    1. we demonstrated that systemic administration of the small molecule CaMKK2 inhibitor, STO-609, to irradiated mice enhanced Hematopoietic stem and progenitor cells (HSPCs)recovery and improved survival. These findings identify CaMKK2 as an important regulator of HSPC regeneration and demonstrate CaMKK2 inhibition is a novel approach to promoting hematopoietic recovery after BM injury. PMID: 28981105
    2. loss of blood-brain barrier proteins, inactivation of survival gene expression such as B-cell lymphoma 2 (Bcl-2) and an increase in inflammatory cytokines in the serum were observed after stroke with CaMKK beta inhibition. We demonstrate that CaMKK beta is neuroprotective in stroke in aged mice PMID: 27305894
    3. CaMKK2 as a molecular rheostat for insulin action. PMID: 27003444
    4. CaMKK2 Inhibits C2C12 Myoblasts Proliferation and Differentiation through AMPK. Overexpression of CaMKK2 Inhibits Muscle Regeneration in Vivo. PMID: 27783047
    5. In summary, we demonstrate a new mechanism of calcium dependent antibacterial strategy in E. coli infected macrophages, which requires autophagy enhancement mediated by activation of CaMKKbeta, ERK, AMPK and FoxO1. PMID: 26703209
    6. CaMKKbeta exerts protective effects on cardiac adaptive energy pooling against pressure-overload possibly through phosphorylation of AMPK and by upregulation of PGC-1alpha. PMID: 25255457
    7. Results demonstrated that genetically inhibiting the CaMKK pathway via CaMKKbeta or CaMK IV is detrimental in the response of female mice to cerebral ischemia PMID: 25331941
    8. a novel function for CaMKK2 in bone remodeling and highlight the potential for its therapeutic inhibition as a valuable bone anabolic strategy that also inhibits OC differentiation in the treatment of osteoporosis. PMID: 23408651
    9. Flow-enhanced sirtuin (SIRT)1 stability is primarily mediated by CaMKKbeta phosphorylation of sirtuin SIRT1 at Ser-27 and Ser-47. PMID: 23754392
    10. amino acid starvation regulates autophagy in part through an increase in cellular Ca(2+) that activates a CaMKK-beta-AMPK pathway and inhibits mTORC1, which results in ULK1 stimulation PMID: 23027865
    11. Results suggest that in PCa progression, CaMKK2 and the AR are in a feedback loop in which CaMKK2 is induced by the AR to maintain AR activity, AR-dependent cell cycle control, and continued cell proliferation. PMID: 22654108
    12. an important function for CaMKK2 in mediating mechanisms that control the amplitude of macrophage inflammatory responses to excess nutrients or pathogen derivatives PMID: 22334678
    13. Suppressed catecholamine-induced induction of gluconeogenic gene expression in CaMKK2 knockout mice leads to improved whole-body glucose homeostasis despite the presence of increased hepatic fat content. PMID: 22240810
    14. a novel role for CaMKK2 as an inhibitor of granulocytic fate commitment and differentiation in early myeloid progenitors PMID: 21816924
    15. conclude that CaMKK2 and AMP-activated protein kinase function in a signaling pathway that participates in the regulation of adiposity PMID: 21862616
    16. findings suggest CaM kinase kinase beta isoforms are required for the formation of hippocampal long-term memory[LTM], but not needed for contextual, trace fear, and passive avoidance LTM PMID: 14586002
    17. Taken together, we conclude that CaMKKbeta has a male-specific function in hippocampal memory formation and we have identified male-restricted transcription occurring during hippocampal memory formation. PMID: 17207577
    18. contrasting phenotypes of the PSD-95 and Camkk2 mutant mice establish the prospect of an independent ,possibly, competing mechanism for the regulation of N-methyl-d-aspartate receptor dependent bidirectional synaptic plasticity PMID: 18082335
    19. CaMKK2/camkiv cascade involved in cerebellar granule cell development and show specifically that Ca(2+)-dependent regulation of BDNF through CaMKK2/CaMKIV is required for this process. PMID: 19605628

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell projection, neuron projection.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Expressed in all tissues tested. A differential expression pattern compared to CAMKK1 is observed in the brain.
  • 数据库链接：

    KEGG: mmu:207565

    STRING: 10090.ENSMUSP00000107297

    UniGene: Mm.289237