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  4. Recombinant Mouse Calpain-2 catalytic subunit (Capn2)

Recombinant Mouse Calpain-2 catalytic subunit (Capn2)

  • 货号：
    CSB-YP004496MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004496MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004496MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004496MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004496MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Capn2Calpain-2 catalytic subunit; EC 3.4.22.53; 80 kDa M-calpain subunit; CALP80; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain-2 large subunit; Millimolar-calpain; M-calpain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-700
  • 氨基酸序列
    S HERAIKYLNQ DYETLRNECL EAGALFQDPS FPALPSSLGY KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG ALGDCWLLAA IASLTLNEEI LARVVPPDQS FQENYAGIFH FQFWQYGEWV EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC SIDITSAADS EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC DSYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED GERGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK NFFLTTRARE RSDTFINLRE VLNRFKLPPG EYVLVPSTFE PHKDGDFCIR VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ LAGEDAEISA FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF ADDELIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
  • 基因功能参考文献：
    1. these findings identify ASAP2 as a new target of Selk-dependent palmitoylation and reveal a new mechanism regulating the efficiency of FcgammaR-mediated phagocytosis. PMID: 27601625
    2. ENaC activity is regulated by calpain-2 proteolysis of MARCKS. PMID: 28468944
    3. These results suggest that TRPV1/Ca(2+)/calpain 2-dependent signaling plays a dominant role in capsaicin-induced ablation of nociceptive terminals and further our understanding of the molecular mechanisms underlying the effects of capsaicin on nociceptors. PMID: 28360106
    4. Calpain-2 modulates pulmonary vascular remodeling in pulmonary arterial hypertension. PMID: 26248159
    5. Dicer is predominantly a Capn2 substrate. Dicer levels were altered in platelets from diabetic mice due to the cleavage of the enzyme by calpain, resulting in loss of function. PMID: 25944670
    6. Data suggest that vitamin D3 up-regulates skeletal muscle fat deposition, up-regulates muscle m-calpain/Capn2 and u-calpain/Capn1 expression, and down-regulates muscle calpastatin expression. PMID: 25520983
    7. Calpain-mediated proteolysis of tropomodulin isoforms TMOD1 and TMOD4 leads to thin filament elongation in dystrophic skeletal muscle. PMID: 24430868
    8. extrasynaptic NMDAR-coupled m-calpain activation is neurodegenerative PMID: 24285894
    9. Down-regulated expression of calpain-2 or talin-1, or pharmacological interference with calpain action, did not affect toxin binding but reduced endocytosis and increased the survival of cells exposed to anthrax lethal toxin. PMID: 24085852
    10. The carboxyl tail of alpha-actinin-4 regulates its susceptibility to m-calpain and thus functions in cell migration and spreading. PMID: 23466492
    11. Calpain-2 was required for FFA-induced expression of CHOP and activation of caspase-12 and caspase-3, thus promoting cell apoptosis in beta-TC3 cells. PMID: 23527285
    12. Calpain2 gene silencing eliminated long-term potentiation and impaired learning and memory. The calpain2 isoform is a critical mediator in learning and memory. PMID: 22903378
    13. Our results indicate that prolonged calpain expression in resident brain cells (neurons, glial and endothelial cells) plays an important role in neuronal degeneration following traumatic brain injury. PMID: 23390828
    14. cleavage of CPEB3 by NMDA-activated calpain 2 accounts for the activity-related translation of CPEB3-targeted RNAs PMID: 22711986
    15. Activation of calpain-1/2 are associated with increased lipid peroxidation and low-density lipoprotein oxidation in the brain of hyperlipidemic mice. PMID: 21836084
    16. Data suggest that that calpain 2 promotes tumor cell growth both in vitro and in vivo through the PI3K-Akt-FoxO-p27(Kip1) signaling cascade. PMID: 22427650
    17. The ability of mouse lens epithelium to maintain lens transparency would be compromised by activation of calpain 2 and associated necrotic cell death. PMID: 21950698
    18. Subtype-selective induction of m-calpain in aorta during atherosclerotic progression is associated with proteolytic disorganization of VE-cadherin and proatherogenic hyperpermeability in cells. PMID: 22064597
    19. Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor 2,3,4 and 9-induced calpastatin in macrophages PMID: 21849499
    20. m-Calpain activation is regulated by its membrane localization and by its binding to phosphatidylinositol 4,5-bisphosphate. PMID: 20729206
    21. determined that semaphorin 3A-mediated calpain activation and growth cone collapse were associated with m-calpain phosphorylation PMID: 20643936
    22. Capn2 plays a role in fundamental mitotic functions, such as the maintenance of sister chromatid cohesion. PMID: 20094891
    23. analysis of a novel role for calpain proteolysis of FAK in regulating adhesion dynamics in motile cells PMID: 20150423
    24. m- or mu-calpain can facilitate repair of damaged plasma membrane. PMID: 19781581
    25. Our findings support a novel role for calpain 2 in limiting membrane protrusions and in regulating lamellipodial dynamics at the leading edge of migrating cells. PMID: 15302585
    26. Gas2DN can increase the activity of calpain and induce degradation of stabilized/mutated beta-catenin PMID: 15817486
    27. calpain-dependent cleavage of amphiphysin I inhibits synaptic vesicle endocytosis during neural hyperexcitation and demonstrate a novel post-translational regulation of endocytosis PMID: 17541403
    28. These results indicate that m-calpain requiring millimolar Ca(2+) level for the full activation plays specific roles in myogenesis. PMID: 18216163
    29. From 8-cell stage to late neurulation stages, CAPN2 is expressed in the cytoplasm and nuclear compartments. In situ hybridization analysis from E8.5 to 14.5 stages indicates high levels of capn2 expression in nervous system, heart and mesodermal tissues PMID: 18415939

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  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Peptidase C2 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:12334

    STRING: 10090.ENSMUSP00000068895

    UniGene: Mm.19306