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  4. Recombinant Mouse Carbonic anhydrase 2 (Ca2)

Recombinant Mouse Carbonic anhydrase 2 (Ca2)

  • 中文名称：
    Recombinant Mouse Carbonic anhydrase 2(Ca2),Yeast
  • 货号：
    CSB-YP004370MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Carbonic anhydrase 2(Ca2),Yeast
  • 货号：
    CSB-EP004370MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Carbonic anhydrase 2(Ca2),Yeast
  • 货号：
    CSB-EP004370MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Carbonic anhydrase 2(Ca2),Yeast
  • 货号：
    CSB-BP004370MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Carbonic anhydrase 2(Ca2),Yeast
  • 货号：
    CSB-MP004370MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ca2
  • Uniprot No.：
  • 别名：
    Ca2; Car2Carbonic anhydrase 2; EC 4.2.1.1; Carbonate dehydratase II; Carbonic anhydrase II; CA-II
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-260
  • 氨基酸序列
    SHHWGYSKH NGPENWHKDF PIANGDRQSP VDIDTATAQH DPALQPLLIS YDKAASKSIV NNGHSFNVEF DDSQDNAVLK GGPLSDSYRL IQFHFHWGSS DGQGSEHTVN KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKIG PASQGLQKVL EALHSIKTKG KRAAFANFDP CSLLPGNLDY WTYPGSLTTP PLLECVTWIV LREPITVSSE QMSHFRTLNF NEEGDAEEAM VDNWRPAQPL KNRKIKASFK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
  • 基因功能参考文献：
    1. It was concluded that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1(+/-) mice remain unexplained. PMID: 28254758
    2. two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty PMID: 26487715
    3. The Na(+)-Cl(-) cotransporter NCC /Carbonic anhydrase II double Knockout mice displayed significant downregulation of pendrin, along with polyuria and salt wasting. PMID: 24429824
    4. CA2 expression is strongly regulated by intracellular pH and by cAMP, suggesting a role for soluble adenylyl cyclase PMID: 24361611
    5. sclerostin upregulated osteocyte expression of carbonic anhydrase 2. PMID: 23737439
    6. Car2, Car3, Car7, and Car15 mRNAs were barely within the detection limits in the mouse harderian gland. PMID: 20820888
    7. Suggest that during amelogenesis CAII controls the differentiation of ameloblasts. Regulation of intracellular pH, followed by activation of the JNK signaling pathway, may be responsible for the effects of CAII on ameloblasts. PMID: 20533306
    8. Both kNBC1-Asp(986) and kNBC1-Asp(988), but not kNBC1-Asp(989), are required for the phosphorylation-induced shift in stoichiometry. A homologous motif (D887ADD) in the carboxy-terminus of the anion exchanger AE1 binds to carbonic anhydrase II. PMID: 12411514
    9. carbonic anhydrases have a functional role specifically in endometrial gland development during postnatal mouse uterine morphogenesis. PMID: 15788756
    10. Results show that the stimulatory effect of the duodenal secretagogue prostaglandin E2 completely depends on carbonic anhydrase II. PMID: 16217040
    11. Altered expression of Car2 and Ncam1 is a downstream event in placental hyperplasia. PMID: 16247769
    12. These results suggest that the loss of function of cytosolic CA II in the stomach of Car2-/- mice leads to up-regulation of an extracellular CA, namely CA IX, which is expressed on the cell surface of the gastric epithelium. PMID: 16396925
    13. Expression in pancreas is restricted to cells within ductal structures and glucagon-positive cells with no colocalization with any insulin-positive cells at any time. PMID: 16586439
    14. In conclusion, skeletal muscle from mice deficient in CA II exhibits altered handling of acid-base challenges and shows normal contractile behavior at normal intracellular pH. PMID: 16601982
    15. identification of serum CA-II as an acute-phase protein; first report that CA-II circulates in the serum following inflammation PMID: 17298961
    16. Immunoblot analyses showed that the levels of carbonic anhydrase II are increased in the brain of Ts65Dn mice, a mouse model for Down syndrome. PMID: 18083150
    17. The metaphyseal widening and changes in the trabecular bone are consistent with osteopetrosis, making the CAII-deficient mouse a valuable model of human disease. PMID: 18175028
    18. CAII deficiency results in a significant decrease in the gene and protein expression of bicarbonate transport proteins from Slc26 gene family - Slc26a4 (pendrin) and Slc26a7. PMID: 18209476
    19. Carbonic anhydrase II is important for duodenocyte acidification by low luminal pH and for eliciting the acid-mediated HCO(3)(-) secretory response. PMID: 19622732

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  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Alpha-carbonic anhydrase family
  • 数据库链接：

    KEGG: mmu:12349

    STRING: 10090.ENSMUSP00000029078

    UniGene: Mm.1186