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Recombinant Mouse Carnitine O-acetyltransferase (Crat)

  • 中文名称：
    Recombinant Mouse Carnitine O-acetyltransferase(Crat),Yeast
  • 货号：
    CSB-YP005940MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Carnitine O-acetyltransferase(Crat),Yeast
  • 货号：
    CSB-EP005940MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Carnitine O-acetyltransferase(Crat),Yeast
  • 货号：
    CSB-EP005940MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Carnitine O-acetyltransferase(Crat),Yeast
  • 货号：
    CSB-BP005940MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Carnitine O-acetyltransferase(Crat),Yeast
  • 货号：
    CSB-MP005940MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Crat
  • Uniprot No.：
  • 别名：
    CratCarnitine O-acetyltransferase; Carnitine acetylase; EC 2.3.1.7; Carnitine acetyltransferase; CAT; CrAT
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-626
  • 氨基酸序列
    MLAFAARTVV KPLGLLKPSS LMKVSGRFKA HQDALPRLPV PPLQQSLDYY LKALQPIVSE EEWAHTKQLV DEFQTSGGVG ERLQKGLERR AKKMENWLSE WWLKTAYLQF RQPVVIYSSP GVILPKQDFV DLQGQLRFAA KLIEGVLDFK SMIDNETLPV EFLGGQPLCM NQYYQILSSC RVPGPKQDSV VNFLKSKRPP THITVVHNYQ FFELDVYHSD GTPLTSDQIF VQLEKIWNSS LQSNKEPVGI LTSNHRNTWA KAYNNLIKDK VNRESVNSIQ KSIFTVCLDK QVPRVSDDVY RNHVAGQMLH GGGSKFNSGN RWFDKTLQFI VAEDGSCGMV YEHAAAEGPP IVALVDHVME YTKKPELVRS PMVPLPMPKK LRFNITPEIK NDIEKAKQNL SIMIQDLDIM MLTFHHFGKD FPKSEKLSPD AFIQVALQLA YYRIYGQACA TYESASLRMF HLGRTDTIRS ASIDSLAFVK GMGDSTVPEQ QKVELLRKAV QAHRAYTDRA IRGEAFDRHL LGLKLQAIED LVSMPDIFMD TSYAIAMHFN LSTSQVPAKT DCVMFFGPVV PDGYGICYNP MEAHINFSVS AYNSCAETNA ARMAHYLEKA LLDMRTLLQN HPRAKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. Responsible for the synthesis of short- and branched-chain acylcarnitines. Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates. Trans-2-enoyl-CoAs and 2-methylacyl-CoAs are poor substrates.
  • 基因功能参考文献：
    1. Studies revealed that ablation of CrAT in myeloid lineage cells did not impact glucose homeostasis, insulin-action, adipose tissue leukocytosis, and inflammation when animals were confronted with a variety of metabolic stressors, including high-fat diet, fasting, or LPS-induced acute endotoxemia. PMID: 28180063
    2. Crat-mediated acetyl group buffering is essential for optimal exercise performance. PMID: 26154055
    3. structural model for L-CPT I (liver CPT I), based on the similarity of this enzyme to the recently crystallized mouse carnitine acetyltransferase PMID: 14711372
    4. the M564G or M564A mutation is sufficient to increase the activity of the enzyme toward medium-chain substrates PMID: 15155726
    5. The predicted full length cDNA sequence of the porcine CRAT gene was characterised and a new 5' variant for dog, rat and mouse was proposed. PMID: 18839069

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  • 亚细胞定位：
    Endoplasmic reticulum. Peroxisome. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
  • 蛋白家族：
    Carnitine/choline acetyltransferase family
  • 数据库链接：

    KEGG: mmu:12908

    STRING: 10090.ENSMUSP00000028207

    UniGene: Mm.20396