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  4. Recombinant Mouse Casein kinase II subunit alpha' (Csnk2a2)

Recombinant Mouse Casein kinase II subunit alpha' (Csnk2a2)

  • 货号：
    CSB-YP526319MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP526319MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP526319MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP526319MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP526319MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Csnk2a2Casein kinase II subunit alpha'; CK II alpha'; EC 2.7.11.1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-350
  • 氨基酸序列
    MPGPAAGSRA RVYAEVNSLR SREYWDYEAH VPSWGNQDDY QLVRKLGRGK YSEVFEAINI TNNERVVVKI LKPVKKKKIK REVKILENLR GGTNIIKLID TVKDPVSKTP ALVFEYINNT DFKQLYQILT DFDIRFYMYE LLKALDYCHS KGIMHRDVKP HNVMIDHQQK KLRLIDWGLA EFYHPAQEYN VRVASRYFKG PELLVDYQMY DYSLDMWSLG CMLASMIFRK EPFFHGQDNY DQLVRIAKVL GTDELYGYLK KYHIDLDPHF NDILGQHSRK RWENFIHSEN RHLVSPEALD LLDKLLRYDH QQRLTAKEAM EHPYFYPVVK EQSQPCAENT VLSSGLTAAR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins.
  • 基因功能参考文献：
    1. Phosphosites notoriously generated by CK2 are not fully abrogated in CK2 alpha/alpha'((-/-)) cells, while some phosphosites unrelated to CK2 are significantly altered. CK2 promotes phosphoproteomics perturbations attributable to kinases other than CK2. PMID: 29119230
    2. We found that C/EBPbeta is phosphorylated by CK2 under AMPK suppression and ER stress, which are important from the viewpoint of the worsening pathological condition of type 2 diabetes, such as decreased insulin secretion and apoptosis of pancreatic beta cells/ PMID: 29448105
    3. data implicate CK2 as a regulator of the Th17/Treg axis and Th17 cell maturation and suggest that CK2 could be targeted for the treatment of Th17 cell-driven autoimmune disorders. PMID: 28468969
    4. This study shows for the first time that LPS inhibits colonic biotin uptake via decreasing membrane expression of its transporter and that these effects likely involve a CK2-mediated pathway. PMID: 28052864
    5. showed that inhibition of CK2 greatly enhanced M3R-stimulated insulin secretion in human islets PMID: 26598688
    6. CK2-regulated IQJC-SCHIP-1 association with AnkG contributes to axon initial segment maintenance. PMID: 25950943
    7. We suggest that L1 stimulates neuritogenesis by activating CK2alpha leading to decreased levels of PTEN and p53 via a novel, L1-triggered and CK2alpha-mediated signal transduction pathway. PMID: 25727698
    8. our results identify CK2 as a novel regulator of TBK1 and IRF3 and suggest that targeting CK2 by small molecular inhibitor may be a viable approach to prevent and treat viral infections. PMID: 25810395
    9. Cytokine-induced activation of NFkappaB in beta cells is dependent on CK2 activity, which phosphorylates NFkappaB p65 at serine 529. PMID: 24366643
    10. we investigated whether PAF could induce NF-kappaB activation and tumour metastasis via enhancing CK2 activity. PMID: 24628121
    11. CK2 phosphorylation of papillomavirus E2 is a negative regulator of viral DNA replication during latent viral replication. PMID: 23637413
    12. These observations identify CK2 as a key regulator of homeostatic properties of the intestinal epithelium that serves to promote wound healing, in part through inhibition of apoptosis under conditions of inflammation. PMID: 22763408
    13. These results indicate CK2 to be linked with unfolded protein response and to resist endoplasmic reticulum stress by activating the XBP-1-GRP78 arm of UPR. PMID: 22768244
    14. results indicate CK2 is crucial for osteoblast differentiation and could be a target for future therapeutics of fracture healing PMID: 21763800
    15. Lack of DNA-PKcs is accompanied by an increase in the protein level of one of the catalytic isozymes of protein kinase CK2, i.e., CK2alpha' and a concomitant increase in CK2 activity. PMID: 21750982
    16. These results reinforce the importance of CK2 and the S422 and T1471 residues for regulation of CFTR and uncover a novel regulation of CFTR by SYK. PMID: 21930781
    17. we report that the kinase CK2 is a novel interaction partner of JAKs and is essential for JAK-STAT activation PMID: 21527517
    18. Data suggest that CK2/PTEN may be a potent target in the control of anaphylaxis and other many PAF-mediated pathologic conditions. PMID: 21531890
    19. Data show that casein kinase 2 alpha (CK2alpha) and CK2alpha' subunits concentrate at the axon initial segment from its initial development, at the same time as pIkappaBalpha and ankyrinG. PMID: 20850541
    20. The CK2 phospho-dependent interaction between TEL2 and the R2TP complex affects phosphatidylinositol 3-kinase-related kinase functions and is essential for mTOR and SMG1 stability in vivo. PMID: 20864032
    21. CK2 and GSK3 phosphorylation on S29 controls wild-type ATXN3 nuclear uptake. PMID: 20347968
    22. this work suggests that SIRT1 is a component of the expansive anti-apoptotic network controlled by CK2; expression of both CK2 and SIRT1 is upregulated with tumorigenesis and downregulated with senescence PMID: 19680552
    23. Protein kinase CK2: signaling and tumorigenesis in the mammary gland. PMID: 11827167
    24. ultraviolet rays increase the specificity of CK2 for p53 at the expense of other cellular CK2 substrates and leading to an overall increase in p53 serine 392 phosphorylation PMID: 12393879
    25. physiological control of G-protein regulation by phosducin-like protein seems to involve phosphorylation by CK2 PMID: 12466282
    26. Dynamic localization/association of subunits may play a role in its cellular regulation PMID: 12485876
    27. results suggest CK2 is a factor required for male germ cell survival; may either promote germ cell survival or repress germ cell death PMID: 12950107
    28. CK2 induces apoptosis in a human prostate cancer xenograft model PMID: 15634760
    29. casein kinase 2 induces PACS-1 binding of nephrocystin and targeting to cilia PMID: 16308564
    30. CK2 regulates NKX3-1 in prostate cells. PMID: 16581776
    31. In epithelial cells, a fraction of CK2 is associated to the plasma membrane and that this localization is controlled by cell-matrix interactions. PMID: 18587631
    32. Ikaros is controlled by the CK2 and PP1 pathways and that a balance between these two signal transduction pathways is essential for normal cellular function and for the prevention of malignant transformation. PMID: 19282287
    33. These studies reveal multisite phosphorylation of IGFBP-3 that both positively and negatively regulate its apoptotic potential. PMID: 19556345

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  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
  • 组织特异性：
    Highly expressed in brain, testis and mature epididymal spermatozoa. Weakly expressed in kidney, liver, lung, spleen and thymus (at protein level).
  • 数据库链接：

    KEGG: mmu:13000

    STRING: 10090.ENSMUSP00000055919

    UniGene: Mm.440348