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  4. Recombinant Mouse Caspase-2 (Casp2)

Recombinant Mouse Caspase-2 (Casp2)

  • 货号：
    CSB-YP004547MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004547MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004547MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004547MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004547MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Casp2; Ich1; Nedd-2; Nedd2Caspase-2; CASP-2; EC 3.4.22.55; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1) [Cleaved into: Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    170-333
  • 氨基酸序列
    G PPCLLVKPCT PEFYQAHYQL AYRLQSQPRG LALVLSNVHF TGEKDLEFRS GGDVDHTTLV TLFKLLGYNV HVLHDQTAQE MQEKLQNFAQ LPAHRVTDSC VVALLSHGVE GGIYGVDGKL LQLQEVFRLF DNANCPSLQN KPKMFFIQAC RGDETDRGVD QQD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.
  • 基因功能参考文献：
    1. A novel function for caspase-2 in myocyte differentiation and myogenesis. PMID: 28765049
    2. Casp2(-/-) mice, which are more susceptible to genomic instability, are limited in their ability to respond to DNA damage and thus carry more damaged cells resulting in accelerated tumourigenesis. PMID: 27518436
    3. BECN1/Beclin 1 binding to viral phosphoprotein (P) induced an incomplete autophagy via activating the pathways CASP2-AMPK-AKT-MTOR and CASP2-AMPK-MAPK by decreasing CASP2. PMID: 28129024
    4. We further generated Casp2(C320S) mutant mice to demonstrate that caspase-2 catalytic activity is essential for its function in limiting aneuploidy. Our results provide direct evidence that the apoptotic activity of caspase-2 is necessary for deleting cells with mitotic aberrations to limit aneuploidy PMID: 27991927
    5. These results provide the first evidence of caspase-2 in regulating haematopoietic stem cell and progenitor differentiation, as well as aneuploidy, in vivo. PMID: 27906175
    6. Study describes a novel pathological process in which caspase-2 cleavage of tau at Asp314 impairs cognitive and synaptic function in animal and cellular models of tauopathies by promoting the missorting of tau to dendritic spines. PMID: 27723722
    7. Caspase-2 deficiency protected mice from diet-induced obesity, metabolic syndrome and nonalcoholic fatty liver disease. PMID: 26890135
    8. The tumor-modulatory effects of Caspase-2 and Pidd1 do not require the scaffold protein Raidd PMID: 25857265
    9. Data show that indoles can effectively suppress acute hepatic inflammation caused by staphylococcal enterotoxin B (SEB) maybe mediated by decreased expression of miR-31 and caspase-2-dependent apoptosis in T cells. PMID: 25706292
    10. Data indicate that the nucleotide-binding domain and leucine-rich repeat containing (NLRP3)-caspase-2 axis drives general endoplasmic reticulum (ER) stress-induced inflammasome activation. PMID: 26341399
    11. these studies elucidate a Caspase-2-p53 signaling network that impacts lung tumorigenesis and chemotherapy response in vivo. PMID: 25301067
    12. data strongly suggest that caspase-2 deficiency leads to increased cellular stress largely because these mice fail to respond to oxidative stress by upregulating their antioxidant defense mechanism PMID: 25531319
    13. These results demonstrate an important role for caspase-2 in regulating proteome and metabolome remodelling during ageing. PMID: 25611376
    14. Caspase-2 is not a suppressor in MYCN-induced neuroblastoma and suggest a tissue and context-specific role for caspase-2 in tumorigenesis. PMID: 25144718
    15. our data point to a critical and novel role for caspase-2 in maintaining bone homeostasis by modulating ROS levels and osteoclast apoptosis during conditions of enhanced oxidative stress that occur during aging PMID: 24691516
    16. Report the novel finding that CASP2 is an endogenous repressor of autophagy. Knockout or knockdown of CASP2 resulted in upregulation of autophagy in a variety of cell types and tissues. PMID: 24879153
    17. Caspase-2 activation is one of the few early molecular events identified in Parkinson disease models. PMID: 25645943
    18. The involvement of caspase 2 and iNOS-mediated apoptotic signaling in nicotine plus high fat diet-induced hepatocellular apoptosis, is reported. PMID: 24830635
    19. Axin expression may facilitate AKI-induced caspase-2 activation followed by activation of PARP and initiation of the necrotic cell death pathway. PMID: 24287941
    20. Data strongly argue against a critical role for caspase-2 in ER-stress-induced apoptosis. PMID: 24292555
    21. caspase-2 is a tumor suppressor in epithelial cancers such as breast cancer, and may be an important genetic marker for aggressive disease and poor prognosis. PMID: 23645210
    22. Loss of caspase-2 accelerates age-dependent alterations in mitochondrial production of reactive oxygen species. PMID: 23504374
    23. caspase-2 is required for the cognitive decline seen in human amyloid precursor protein transgenic mice. PMID: 23748737
    24. The findings suggest that loss of caspase-2 serves as a key function in maintaining genomic integrity, during cell proliferation and following DNA damage. PMID: 22498700
    25. Brucella abortus strains RB51 and S2308-induced BMDC cell death was regulated by caspase-2 PMID: 22927979
    26. Caspase-2 showed tumor suppressive capacity in response to aberrant c-Myc expression, which did not rely on PIDD, the BH3-only protein Bid or the death receptor ligand Trail. PMID: 22595758
    27. IRE1alpha regulates translation of a proapoptotic protein, Caspase-2, through terminating microRNA biogenesis, and noncoding RNAs are part of the ER stress response PMID: 23042294
    28. caspase-2 gene deficiency combined with hypothermia provided enhanced neuroprotection as compared with hypothermia alone PMID: 22322383
    29. Activation of neuronal Casp2 and induction of this activity depends on expression of receptor-interacting protein RAIDD, but is independent of p53-inducible protein with a death domain (PIDD) expression. PMID: 22515271
    30. Casp2-deficient mice subjected to hypoxia-ischemia at postnatal day 9 present significantly lower cerebral infarction, reduced white matter injury, and reduced Casp3 activation in the thalamus and hippocampus. PMID: 21674587
    31. ras-induced down-regulation of caspase-2 represents a novel mechanism by which oncogenic Ras protects malignant intestinal epithelial cells from anoikis PMID: 21903589
    32. caspase-2 deficiency may play a role in promoting marrow adiposity under stress or disease conditions PMID: 21538476
    33. a novel function of caspase-2 in mitochondrial oxidative stress-induced apoptosis in neurons cultured from young adult mice. PMID: 21216964
    34. caspase 2, an initiator caspase, localizes to the centrosomes in mitotic primary mouse embryonic fibroblasts, irrespective of DNA mismatch repair status and ultraviolet radiation B treatment. PMID: 20022306
    35. mechanism for caspase-2 nuclear import PMID: 12477715
    36. Caspases other than caspases 2 and 9 can promote cytochrome c release and initiate Bcl-2-regulated apoptosis. PMID: 15210727
    37. caspase-2 and caspase-8 activation have roles in the mitochondrial apoptotic pathway induced by ceramide or etoposide PMID: 15262979
    38. demonstrate the inducible assembly of a novel protein complex consisting of caspase-2, TRAF2, and RIP1 that activates NF-kappaB and p38 MAPK PMID: 15590671
    39. These results suggest that caspase-2 activation mediated by p53 is an important pathway involved in the mitochondrial release of apoptosis-inducing factor in response to cisplatin injury. PMID: 15983031
    40. Caspase-2 contributes to P53-induced protein with a death domain-mediated cell death, but that it is not the sole effector of this pathway. PMID: 16183742
    41. caspase-2 is an early checkpoint for apoptosis initiation in primary neurons subjected to serum deprivation. PMID: 16215683
    42. This approach unambiguously identifies the apical caspase activated in response to apoptotic stimuli, and establishes caspase-2 as a proximal mediator of heat shock-induced apoptosis. PMID: 16362053
    43. caspase 2 plays an important role upstream of the mitochondria in germ cell apoptosis during the first phase of spermatogenesis PMID: 16481596
    44. caspase-2 deficiency affects aging in the mice PMID: 17188333
    45. These results indicate a role for GSK-3beta in ceramide-induced apoptosis, in which GSK-3beta acts downstream of PP2A and the PI 3-kinase-Akt pathway, and upstream of caspase-2 and caspase-8. PMID: 17666435
    46. our data indicate that caspase 2 and JNK-mediated intrinsic pathway signaling is one of the mechanisms involved in age-related increase in muscle cell apoptosis. PMID: 18461459
    47. results revealed the presence of caspase-2(L), caspase-9, and caspase-3 in their zymogen and cleaved forms in the oocyte before meiosis resumption PMID: 19035350
    48. loss of caspase-2 results in an increased ability of cells to acquire a transformed phenotype and become malignant, indicating that caspase-2 is a tumor suppressor protein. PMID: 19279217
    49. Results suggest that at least one alternative PIDDosome-independent mechanism of caspase-2 activation exists in mammals in response to DNA damage. PMID: 19364921
    50. Caspase-2 phosphorylated at S135 binds 14-3-3zeta, thus preventing C2 dephosphorylation. PMID: 19531356

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  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    High level expression seen in the embryonic CNS, liver, lung, kidney, small intestine, and hair follicles of vibrissae. Moderate expression seen in the skin, oral mucosa, skeletal muscle, submandibular gland and thymus. In the adult, it is highly expresse
  • 数据库链接：

    KEGG: mmu:12366

    STRING: 10090.ENSMUSP00000031895

    UniGene: Mm.3921