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  4. Recombinant Mouse Caspase-4 (Casp4), partial

Recombinant Mouse Caspase-4 (Casp4), partial

  • 货号：
    CSB-YP004549MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004549MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004549MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004549MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004549MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CASP-11; CASP-4; CASP11; CASP4; CASP4_MOUSE; Caspase 11; Caspase 11 apoptosis related cysteine peptidase; Caspase 11 apoptosis related cysteine protease; Caspase 4; Caspase 4 apoptosis related cysteine peptidase; Caspase-11; Caspase-4 subunit p20; CASPL; Ich 3; Ich3; ICH3 protease; Protease ICH-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    partial
  • 表达区域：
    81-266
  • 氨基酸序列
    PGSHHGEANL EMEEPEESLN TLKLCSPEEF TRLCREKTQE IYPIKEANGR TRKALIICNT EFKHLSLRYG ANFDIIGMKG LLEDLGYDVV VKEELTAEGM ESEMKDFAAL SEHQTSDSTF LVLMSHGTLH GICGTMHSEK TPDVLQYDTI YQIFNNCHCP GLRDKPKVII VQACRGGNSG EMWIRE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS, as well as infection with Gram-negative bacteria. Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1. Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators. Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity. Involved in cell death induced by endoplasmic reticulum stress. Activated by direct binding to LPS without the need of an upstream sensor. Does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.
  • 基因功能参考文献：
    1. Together, these data suggest that caspase-11/IL-1alpha pathway plays an important role in defending against Klebsiella pneumoniae by recruiting neutrophils in the early stage of infection. PMID: 28939441
    2. Results indicate functional differences between human caspase-4 and murine caspase-11 (caspase 4). PMID: 29339744
    3. Data suggest that Brucella melitensis effector protein TcpB suppresses caspase-4/11--mediated inflammation, subverting inflammasome activation in mouse macrophages; TcpB induces ubiquitination/degradation of caspase-4/11; TcpB suppresses caspase-4/11--mediated pyroptosis and proinflammatory responses induced by lipopolysaccharide. PMID: 29061850
    4. systemic exposure to the bacterial endotoxin lipopolysaccharide (LPS) causes severe endothelial pyroptosis that is mediated by the inflammatory caspases, human caspases 4/5 in human ECs, or the murine homolog caspase-11 in mice in vivo. PMID: 28990935
    5. Caspase-l, Caspase-11 and Dectin-1/syk pathway are involved in the IL-1beta generation in fungal keratosis. PMID: 28167474
    6. These data indicate that the caspase-11-mediated innate immune response plays a crucial role in defending against Acinetobacter baumannii. PMID: 28760936
    7. Hyperactive dendritic cells induce potent adaptive immune responses and are elicited by caspase-11, an enzyme that binds oxidized phospholipids and bacterial lipopolysaccharide. PMID: 27103670
    8. gasdermin D is essential for caspase-11-dependent pyroptosis and interleukin-1beta maturation PMID: 26375259
    9. But not in Casp11(-/-), Panx1(-/-), or P2x7(-/-) mice. PMID: 26572062
    10. active caspase-11 leads to a drop of intracellular potassium levels, which is necessary to activate NLRP3. PMID: 26173909
    11. These results suggest a role for other caspase-11 effector mechanisms such as pyroptosis in protection against intestinal inflammation. PMID: 24850431
    12. Caspase-11 limits the response of CD8+ T-cells to low-abundance and low-affinity antigens. PMID: 25980012
    13. These data show that both Candida albicans Sap2 and Sap6 can activate caspase-11 through type I interferon (IFN) production. PMID: 25712931
    14. these data suggest that IFN-gamma-mediated caspase-11 expression has a key role maintaining intestinal epithelial barrier integrity in vivo during experimentally induced acute colitis. PMID: 25548224
    15. The caspase-4/11 inflammasome also governs activation of the proinflammatory cytokine, interleukin (IL)-18, in response to intracellular and extracellular enteric pathogens. PMID: 25121752
    16. Lipopolysaccharides may be sensed in the cytosol of cells by a noncanonical inflammasome pathway, resulting in the activation of the cysteine protease caspase-11. [Review] PMID: 25347553
    17. identify caspase-11 as a critical factor protecting the host during acute dextran sulfate sodium-induced colonic injury and inflammation but not during chronic inflammation and tumorigenesis PMID: 25414099
    18. function of caspase-4/5/11 represents a new mode of pattern recognition in immunity and also an unprecedented means of caspase activation PMID: 25119034
    19. caspase-4 can support activation of caspase-1 and secretion of IL-1beta and IL-18 in response to priming signals (LPS or Pam3CSK4) alon PMID: 24879791
    20. a cluster of small interferon-inducible GTPases, the so-called guanylate-binding proteins, is required for the full activity of the non-canonical caspase-11 inflammasome during infections with vacuolar Gram-negative bacteria PMID: 24739961
    21. IFN-inducible guanylate binding protein (Gbp) proteins stimulate caspase-11-dependent, cell-autonomous immunity in response to cytoplasmic lipopolysaccharides. PMID: 24715728
    22. Emerging data point to a cascade of events during which caspase-11 promotes and corroborates caspase-1 activity through NLRP3 inflammasomes. Caspase-11 is also capable of independently inducing pyroptotic cell death.[Review] PMID: 23870309
    23. virulent, but not avirulent, bacteria trigger a rapid caspase-11-dependent innate immune response important for host defense. PMID: 23762026
    24. review gathers together studies on both the mechanisms and implications of caspase-11-mediated noncanonical inflammasome activation, and discuss the emerging importance of this pathway in regulating host defense against intracellular bacterial pathogens PMID: 24037676
    25. study reports that caspase-11 is required for innate immunity to cytosolic, but not vacuolar, bacteria PMID: 23348507
    26. TLR4- and TRIF-induced caspase-11 synthesis is critical for noncanonical Nlrp3 inflammasome activation in macrophages infected with enteric pathogens. PMID: 22898816
    27. non-canonical caspase-11 activation contributes to macrophage death during S. typhimurium infection PMID: 22895188
    28. Caspase-11 activation via the TLR4-TRIF-IFNbeta pathway synergizes with the NLRP3 pathway to coordinate caspase-1-dependent IL-1beta and IL-18 secretion and also leads to caspase-1-independent cell death. PMID: 22819539
    29. found that, despite extensive backcrossing into a C57BL/6 background, c-IAP1(-/-) animals are also deficient in caspase 11 PMID: 22332634
    30. data highlight a unique pro-inflammatory role for caspase-11 in the innate immune response to clinically significant bacterial infections PMID: 22002608
    31. PARP-1, as a transcriptional cofactor for NF-kappaB, regulates the induction of caspase-11 at a transcriptional level. PMID: 21530491
    32. TNFA deletion alters apoptosis as well as caspase 3 and 4 expression during otitis media. PMID: 21269505
    33. 11 is an essential molecule in NO-independent apoptotic pathway of activated astrocytes. PMID: 11902113
    34. gene expression in response to LPS and interferon-gamma requires NF-kappaB and Stat1 binding PMID: 12198138
    35. In a mouse model of ALS neurodegeneration, inflammatory responses, and disease onset and progression in SOD1 G93A transgenic mice were not altered by the ablation of caspase-11 gene PMID: 12843244
    36. the neurotoxic effects of LPS on nigral dopaminergic neurons are mediated by microglial activation, interleukin-1beta, and caspase-11 expression in mice PMID: 15383538
    37. Caspase-11 regulates cell migration during inflammation. PMID: 17293856
    38. Our results suggest that neuroinflammation related to the caspase-11 cascade in the striatum regulates differentiation of neural stem cells in the SVZ of our mouse model of PD. PMID: 17963913
    39. Caspase-11 plays a critical role in TPEN-induced apoptosis. PMID: 18474237
    40. Interferon beta induces mature dendritic cell apoptosis through caspase-11/caspase-3 activation. PMID: 19531658
    41. Ask1 regulates the expression of caspase-11 following LPS stimulation PMID: 19695249

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Widely expressed, including in thymus, lung and spleen (at protein level). Very low levels, if any, in the brain.
  • 数据库链接：

    KEGG: mmu:12363

    STRING: 10090.ENSMUSP00000027012

    UniGene: Mm.1569