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  4. Recombinant Mouse Cbp/p300-interacting transactivator 2 (Cited2)

Recombinant Mouse Cbp/p300-interacting transactivator 2 (Cited2)

  • 货号：
    CSB-YP005448MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005448MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005448MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005448MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005448MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cited2; Mrg1; Msg2Cbp/p300-interacting transactivator 2; MSG-related protein 1; MRG-1; P35srj
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-269
  • 氨基酸序列
    MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP TAGHQMNGTN QHFRDCNPKH SGGSSTPGGA GGSGTPGGSG GTSGGAGGSS AGGSGGGSTM PASVAHVPAA MLPPNVIDTD FIDEEVLMSL VIEMGLDRIK ELPELWLGQN EFDFMTDFVC KQQPSRVSC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional coactivator of the p300/CBP-mediated transcription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region.
  • 基因功能参考文献：
    1. Knockdown of CITED2 led to a decreased interaction of p53 with its inhibitor MDM2, which results in increased amounts of total p53 protein. Data indicate that CITED2 functions in pathways regulating p53 activity. PMID: 29072699
    2. Data suggest that tumor suppressor gene Cited2 is direct target of microRNA-182-5p; here, carcinogenic solvent trichloroethylene (TCE) up-regulates expression of microRNA-182-5p in liver; TCE-induced hepatocyte cell proliferation is mediated by microRNA-182-5p overexpression and down-regulation of Cited2 expression. PMID: 28013219
    3. ectopic CITED2 expression at the onset of the reprogramming process in combination with the reprogramming factors promotes a complete and homogeneous conversion of somatic cells into induced pluripotent stem cells PMID: 28684114
    4. This study demonstrated that Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity in mice. PMID: 27307230
    5. Study showed a novel function of miR-200b and CITED2 in high glucose-induced unfolded protein response and ER stress, suggesting that miR-200b and CITED2 are critical for ER homeostasis and neural tube defect formation in the developing embryo. PMID: 26450995
    6. MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2. PMID: 26240138
    7. Cited2 is required for and enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells. PMID: 25377420
    8. Cited2 is required for the maintenance of adult hematopoietic stem cell glycolytic metabolism likely through regulating Pdk2, Pdk4, lactate dehydrogenases B and D, and Akt activity. PMID: 24083546
    9. Cited2 in sinusoidal trophoblast giant cells and syncytiotrophoblasts is likely to have a non-cell autonomous role in patterning of the pericytes associated with the embryonic capillaries. PMID: 24803182
    10. a deletion of Cited2 in mESCs results in abnormal mitochondrial morphology and impaired glucose metabolism, which correlates with a defective cell fate decision. PMID: 24265312
    11. CITED2 is phosphorylated by MAPK1 in vitro at T166, and that MAPK1 activation enhances the coactivation function of CITED2 but not of CITED2-T166N. PMID: 23082118
    12. Cited2 controls ESC pluripotency and differentiation via direct regulation of Oct4 gene expression. PMID: 22761414
    13. This study demonistrated that loss of hepatic CITED2 function suppresses gluconeogenesis in diabetic mice, suggesting it as a therapeutic target for hyperglycemia PMID: 22426420
    14. Our results suggest that Cited2 regulates hematopoietic stem cell quiescence through both HIF-1-dependent and HIF-1-independent pathways PMID: 22308296
    15. Loss of Cited2 causes congenital heart disease by perturbing left-right patterning of the body axis. PMID: 21224256
    16. Lipopolysaccharide induces CITED2 expression via nuclear factor (NF)-kappaB in J774 cultured cells and bone-marrow-derived macrophages. PMID: 21098220
    17. High-fat diet reduces Pitx2c levels in Cited2-deficient embryos and enhance penetrance of left-right patterning defects. PMID: 20566713
    18. Conditional deletion in the adult mouse results in loss of hematopoietic stem cells causing multilineage bone marrow failure and death PMID: 19951693
    19. investigate the function of Cited2 during mouse embryogenesis PMID: 11823447
    20. essential role of cited2, a negative regulator for HIF-1alpha in heart development and neurulation PMID: 12149478
    21. MRG1 and GK are cannabinoid-regulated genes and that they may be involved in the vascular and hypothalamic effects of cannabinoids, respectively. PMID: 12237329
    22. interactions among TFAP2A, CITED2, and p300/CBP are necessary for TFAP2A-mediated transcriptional activation and for normal neural tube and cardiac development. PMID: 12586840
    23. Results provide genetic evidence that Cited2 controls the expression of INK4a/ARF and fibroblast proliferation, at least in part via the polycomb-group genes Bmi1 and Mel18. PMID: 14560011
    24. CIT-ED2 is a coactivator of PPARalpha and both proteins may participate in signaling cascades of hypoxic response and angiogenesis PMID: 15051727
    25. We propose that an abnormal Nodal-Pitx2c pathway represents a unifying mechanism for the cardiovascular malformations observed in Cited2(-/-) mice, and that such malformations may be the sole manifestation of a laterality defect. PMID: 15475956
    26. Mutants cause heart defects by disruption of normal left-right patterning and direct loss of Cited2 expression in cardiac tissues. PMID: 15750185
    27. Cited2 is required for normal placental development and vascularisation, and hence for embryo viability PMID: 16579983
    28. highly hypoxic cardiac regions in mouse embryos corresponded to sites of defects in Cited2(-/-) embryos and defects of outflow tract, interventricular septum, cardiac vasculature, and hyposplenia were largely rescued by HIF-1alpha haploinsufficiency PMID: 17022961
    29. Adrenal development is initiated by Cited2 and WT1 through modulation of Sf-1 dosage. PMID: 17537799
    30. The expression pattern of Cited2 overlaps that of Cited1, but its deletion, either alone or in combination with Cited1, does not disrupt epithelial differentiation of the metanephric mesenchyme. PMID: 17615577
    31. novel role of Cited2 in the maintenance of hematopoietic homeostasis during embryogenesis a PMID: 17644732
    32. Cited2, a coactivator of HNF4alpha, is essential for liver development PMID: 17932483
    33. In fibroblasts as well as in breast cancer cells, FOXO3a inhibits HIF1-induced apoptosis by stimulating the transcription of CITED2. PMID: 18158893
    34. This study shows that E17.5 to E18.5 Cited2-null lungs had significantly reduced terminal sac space due to an altered differentiation of type I and type II alveolar epithelial cells. PMID: 18358466
    35. Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency. PMID: 18440989
    36. An abnormal corneal-lenticular stalk was characteristic of Cited2(-/-) developing eyes, a feature reminiscent of Peters' anomaly, which can be rescued by increased Pax6 gene dosage in Cited2(-/-) embryonic eyes. PMID: 18653562
    37. These results suggest that the gain of Cited2 function counteracts glucocorticoid-induced muscle atrophy through inhibition of proteolysis mediated by p300-dependent gene transcription. PMID: 19032942
    38. CITED2 functions during sex determination and early gonad development. PMID: 19457926
    39. Cited2 may play a role in corneal morphogenesis in part through modulating the expression of Pax6 and Klf4 PMID: 19632219

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CITED family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:17684

    STRING: 10090.ENSMUSP00000038405

    UniGene: Mm.272321