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  4. Recombinant Mouse Cell division cycle protein 20 homolog (Cdc20)

Recombinant Mouse Cell division cycle protein 20 homolog (Cdc20)

  • 货号：
    CSB-YP864334MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP864334MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP864334MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP864334MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP864334MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc20Cell division cycle protein 20 homolog; mmCdc20; p55CDC
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-499
  • 氨基酸序列
    MAQFVFESDL HSLLQLDAPI PNAPVARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP GKSSSKVQTT PSKPGGDRFI PQRSASQMEV ASFLLSKENQ PEDRGTPTKK EHQKAWSLNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWAPLQT FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLGLT MSPDGATVAS AAADETLRLW RCFEMDPALR REREKASVAK SSLIHQGIR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
  • 基因功能参考文献：
    1. Cdc20 hypomorphism causes chromatin bridging and chromosome misalignment, revealing a requirement for Cdc20 in efficient sister chromosome separation and chromosome-microtubule attachment. PMID: 20956380
    2. The physiologically effective threshold level of Cdc20 is high for female meiosis I. PMID: 20941357
    3. Results indicate that Cdc20 also contributes to post-anaphase activation of the APC/C. PMID: 20053638
    4. This cell division cycle protein is a key regulator of the cell cycle. PMID: 12710943
    5. The seven tandem WD motifs of Cdc20 they are required for speriolin binding and for localization of Cdc20 to the centrosomes and nucleus, suggesting that speriolin might regulate or stabilize the folding of Cdc20 during meiosis in spermatogenic cells PMID: 15280373
    6. Data suggest that Mad2 and BubR1 must cooperate to inhibit Cdc20 activity. PMID: 16600213
    7. Cdc20 is degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box. PMID: 16878123
    8. Cdc20 and securin double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin in preventing mitotic exit PMID: 17325031
    9. Expression of the cdc20 gene is down-regulated by zif268 in neuronal cells; altered expression of proteasome-regulatory genes following zif268 induction may be a key component of long-lasting CNS plasticity. PMID: 17372985
    10. Cdc20 is required for the anaphase onset of the first meiosis but not the second meiosis in mouse oocytes PMID: 18000406
    11. findings suggest a novel function of HSF1 frequently overexpressed in cancer cells, to inhibit APC/C activity by interacting with Cdc20, and to result in aneuploidy development and genomic instability PMID: 18059335
    12. Data show that the BubR1 N terminus binds to Cdc20 to inhibit APC/C activity in interphase, thereby allowing accumulation of cyclin B in G(2) phase prior to mitosis onset. PMID: 19154723
    13. Results indicate that the spindle assembly checkpoint-mediated inhibition of Cdc20 is an important tumor-suppressing mechanism. PMID: 19528295

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    WD repeat CDC20/Fizzy family
  • 数据库链接：

    KEGG: mmu:107995

    STRING: 10090.ENSMUSP00000006565

    UniGene: Mm.261764