Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Chromobox protein homolog 5 (Cbx5)

Recombinant Mouse Chromobox protein homolog 5 (Cbx5)

  • 货号：
    CSB-YP730749MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP730749MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP730749MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP730749MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP730749MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cbx5
  • Uniprot No.：
  • 别名：
    Cbx5; Hp1aChromobox protein homolog 5; Heterochromatin protein 1 homolog alpha; HP1 alpha
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-191
  • 氨基酸序列
    MGKKTKRTAD SSSSEDEEEY VVEKVLDRRM VKGQVEYLLK WKGFSEEHNT WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSEGNKR KSSFSNSADD IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKESAK S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • 基因功能参考文献：
    1. results reveal that ADNP, via the recruitment of HP1 and CHD4, regulates the expression of genes that are crucial for maintaining distinct cellular states and assures accurate cell fate decisions upon external cues PMID: 29795351
    2. HP1-alpha oligomerization is critical to the maintenance of heterochromatin and the tunable dynamics of this HP1 isoform. PMID: 26238434
    3. demonstrate an actin-driven differential modulation of core histone H2B and heterochromatin HP1alpha protein dynamics with chromatin PMID: 26359759
    4. the molecular behavior of HP1alpha throughout the cell cycle PMID: 25036950
    5. There is a functional link between prelamin A, HP1alpha, chromatin remodeling, DNA repair, and early senescence in Zmpste24-deficient mice, suggesting a potential therapeutic strategy for laminopathy-based premature aging via the intervention of HP1alpha. PMID: 24584199
    6. the TIF1beta-HP1 system functions as a critical repressive machinery that targets genes not normally activated in the hematopoietic compartment, thereby maintaining the transcriptional signature specific to HSCs. PMID: 24527388
    7. HP1 mobility in apoptotic cells appeared to be unique. PMID: 23023195
    8. Elimination of HP1alpha by siRNA interfered with centromere relocation from heterochromatin surrounding NPBs to pro-chromocenters at the two-cell stage but did not affect preimplantation develoment to the blastocyst stage. PMID: 22622086
    9. Depletion of Senp7 delocalizes HP1 alpha from pericentric heterochromatin without affecting H3K9me3 levels. PMID: 22388734
    10. The interplay between MU2 and HP1a is dynamic and may be different in euchromatin and heterochromatin during DNA break recognition and repair. PMID: 21966530
    11. KAP1 and HP1 tether activation-induced cytidine deaminase (AID) to H3K9me3 residues at the donor switch region, thus linking AID to epigenetic modifications during class switch recombination. PMID: 21746811
    12. Our findings suggest that H4K20me3 and HP1-alpha are markers for cell type commitment that can be triggered by developmental or cell context, independently of the differentiation process PMID: 21576353
    13. The HP1alpha recruitment at early stages of the DDR involves p150CAF-1 and is critical for proper DNA damage signaling and repair. PMID: 21464229
    14. Hp1a was multiply phosphorylated at N-terminal serine residues (S11-14) in human and mouse cells and this phosphorylation enhanced its affinity for K9-methylated histone H3. PMID: 21245376
    15. post-gastrulation development, but not for Sertoli cell functions during spermatogenesis. PMID: 21163256
    16. The 3sFRET microscopy method was then applied to study the interactions of the dimeric transcription factor C/EBPalpha (expressing mTFP or mVenus) with the heterochromatin protein 1alpha (HP1alpha, expressing tdTomato) in live-mouse pituitary cells. PMID: 20713013
    17. quantitative description of HP1alpha/beta in terms of its concentration, diffusion coefficient, kinetic binding, and dissociation rate constants PMID: 19948116
    18. The scFv fragments can recognize both CD motifs of HP1 and Polycomb (Pc) in vitro and, when expressed intracellularly, interact with and dislodge the HP1 protein(s) from their heterochromatin localization in vivo. PMID: 11956312
    19. Data provide evidence for nuclear compartmentalization of transcriptional intermediary factor 1 beta (TIF1beta) that is regulated during cell differentiation through a mechanism that requires heterochromatin protein 1 interaction. PMID: 12154074
    20. HP1alpha has separate domains that recognize histone H3 tails and RNA PMID: 12231507
    21. Data suggest that self-interaction through the chromo shadow domain of heterochromatin protein 1 alpha is crucial for recruitment of SUV39H1 onto nucleosomes. PMID: 12565857
    22. Molecular explanation for the functional link of these epigenetic silencing processes mediated by Su(z)12 PMID: 14570930
    23. HP1alpha interacts with the preRC proteins ORC1, ORC2 and CDC6; results together indicate that HP1alpha plays a role in mitosis and cytokinesis PMID: 16950245
    24. Knockdown of SUZ12 in human cells caused a reduction in H3K27me3 and H3K9me3, and altered the distribution of HP1 alpha. PMID: 17406994
    25. epigenetic regulations orchestrated by HP1 isoforms, are critical for permanent cell cycle exit during neuronal differentiation PMID: 17627279
    26. the Suv39h-HP1 pathway is not essential for enrichment and mitotic protection of cohesin at centromeres in mammalian cells PMID: 18075750
    27. TRIM28 interaction with HP1 is absolutely required for the primer binding site -dependent restriction of murine leukemia virus in the F9 EC cell line. PMID: 18287239
    28. H3K9me3 and HP1alpha recruitment to retrotransposons occurs independent of DNA methylation in ES cells and G9a promotes DNA methylation independent of its HMTase activity PMID: 18818693

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Chromosome. Chromosome, centromere.
  • 数据库链接：

    KEGG: mmu:12419

    STRING: 10090.ENSMUSP00000104441

    UniGene: Mm.262059