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  4. Recombinant Mouse Ciliary neurotrophic factor (Cntf)

Recombinant Mouse Ciliary neurotrophic factor (Cntf)

  • 货号：
    CSB-YP005683MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005683MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005683MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005683MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005683MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CntfCiliary neurotrophic factor; CNTF
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-198
  • 氨基酸序列
    MAFAEQSPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNISL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLTLQVSAFA YQLEELMALL EQKVPEKEAD GMPVTIGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRVIS SHHMGISAHE SHYGAKQM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
  • 基因功能参考文献：
    1. Study provides new insights into the role of CNTF on the migration of corneal epithelial stem/progenitor cells and its inherent mechanism of up-regulation of matrix metalloproteinases through the Akt signaling pathway. PMID: 27174608
    2. data suggest that All-trans retinoic acid administration upregulates CNTF expression in retinal pigment epithelial cells. PMID: 28589680
    3. Our data reveal that haematopoietic cell-derived CNTF has roles in regulating BM B cell lymphopoiesis and both trabecular and cortical bone, the latter in a sex-dependent manner. PMID: 26487326
    4. CNTF is upregulated in the corticospinal tract after unilateral pyramidotomy, which likely triggers sprouting of uninjured axons. PMID: 26598325
    5. Results suggest that slowly up-regulated CNTF in the subventricular zone mediates stroke-induced neurogenesis and is counteracted by inflammation PMID: 22960105
    6. Ciliary neurotrophic factor activation of astrocytes decreases spreading depolarization susceptibility and increases potassium clearance PMID: 25092804
    7. STAT3 can activate CNTF transcription because it bound to its promoter and FAK antagonist-induced CNTF was reduced by blocking STAT3. PMID: 23693126
    8. data indicate that muscle cells secrete abundant TIMP-1, MCP-1, and CNTF, and that of these, only CNTF has the ability to suppress osteoblast function and gene expression in a similar manner to myotube-conditioned medium PMID: 24721701
    9. Report intravitreal transplantations of modified neural stem cells as cell based delivery system for CNTF in mouse model of photoreceptor degeneration. PMID: 24167317
    10. CNTF activates hypothalamic urocortin 1-expressing neurons both in vitro and in vivo. PMID: 23626705
    11. CNTF may play a role in the process of remyelination by inducing the MOG expression. PMID: 23443463
    12. Data indicate that aspirin increased mRNA and protein expression of CNTF in primary mouse and human astrocytes in a dose- and time-dependent manner. PMID: 23653362
    13. These studies demonstrate that endogenous CNTF and LIF act centrally to protect axons from acute inflammatory destruction via an oligodendrocyte-independent mechanism. PMID: 23077604
    14. Both CNTF and its receptor, CNTF-Ralpha, are expressed in skeletal muscle. PMID: 22971093
    15. study observed that CNTF increased the number of IFN-gamma producing CD4 T cells results indicate CNTF activates the same tripartite receptors in mouse and human cells PMID: 22986012
    16. CNTF acts as a chemoattractant and participates in the recruitment of endogenous progenitors during myelin repair PMID: 23407977
    17. Contact-mediated integrin signaling between neurons and astrocytes normally represses CNTF expression; neuronal dysfunction causes a rapid protective response by the brain in stroke. PMID: 22764235
    18. results demonstrate that endogenous CNTF in the CNS is a pivotal regulator of depressive-like and anxiety-like behavior via effects on GABAergic neurons and associated serotonergic neurotransmission. PMID: 22266927
    19. CNTF decreased TH enzyme levels, thereby increasing the specific activity of the enzyme PMID: 22372951
    20. In the retina of CNTF(-/-) mice, basal GFAP expression was reduced, but unexpectedly, injury-induced upregulation in activated Muller cells was increased during the first 3 days after lesion. PMID: 20091786
    21. CNTF is expressed locally in bone and plays a unique role in female mice as an inhibitor of trabecular bone formation and in male mice as a stimulus of cortical growth. PMID: 20157807
    22. These findings indicate that CNTF is necessary for a sprouting response and thus enhances the size of motor units in skeletal muscles of mouse model of type III/IV spinal muscular atrophy. PMID: 20022887
    23. CNTF-immunoreactive astrocytes were exclusively found in white matter structures. CNTF receptor alpha-expressing astrocytes were found in gray matter areas. PMID: 11870876
    24. a major protective factor in demyelinating CNS disease; a neurotrophic cytokine as modulator in neuroinflammation PMID: 12042814
    25. Astroglial expression of the P-glycoprotein is controlled by intracellular CNTF PMID: 12150717
    26. CNTF affects adipocyte gene expression, and the specific receptor for this cytokine is induced in rodent models of obesity/type II diabetes PMID: 12424252
    27. CNTF is an important cytokine in demyelinating disease and as an upstream regulator of FGF-2 production in astrocytes during early remyelination. PMID: 12828933
    28. CNTF is required for the development and maintenance of the mature astrocyte phenotype and is part of the complex regulatory network modulating lesional glial reactivity after optic nerve injury PMID: 12843240
    29. In ciliary neurotrophic factor lacZ-knock-in mice, reactivity is found in ensheathing cells of the olfactory nerve layer, and in cells of the glomerular, external plexiform and granular layers of the olfactory bulb. PMID: 12849744
    30. In CNTF(-/-) mouse mutants, activation of STAT3 signaling was delayed by 10-12 hours in animals with facial nerve lesions. Application of CNTF to the transected nerve restored rapid STAT3 activation in CNTF-deficient animals. PMID: 14550774
    31. Endogenous CNTF regulates adult neurogenesis by increasing proliferation of neural stem cells and/or precursors. Alternatively, CNTF could maintain cells longer in the S-phase PMID: 14552871
    32. CNTF-CNTFRalpha activates the JAK/STAT pathway leading to enhanced Cx43 expression and intercellular coupling PMID: 15342787
    33. The evidence was provided for a role of CNTF in the selective modulation of adipocyte endocrine function which may have important implications for the regulation of energy homeostasis. PMID: 15353177
    34. We have analyzed the target cell types and signaling events for CNTF in the developing retina. PMID: 15525763
    35. demonstrate a novel region-specific role for CNTF/leukemia inhibitory factor/gp130 signaling in the development of the germinal layers of the embryonic telencephalon PMID: 15634701
    36. In cntf/lif/ct-1 triple-knock-out mice, various degrees of muscle fiber type grouping are found, showing that denervation & reinnervation had occurred. CNTF, LIF, & CT-1 have distinct functions for motoneuron survival and function. PMID: 15716414
    37. Disruption of CNTF by mediators of oxidative stress may contribute to the neuronal damage observed in neurodegenerative diseases and significantly affect the utility of CNTF-like factors as therapeutic agents in preventing nerve cell death. PMID: 15748169
    38. results suggest that CNTF can rapidly decrease muscle Ca2+ channel currents by promoting inactivation, probably through an intracellular PKC-dependent mechanism PMID: 16274672
    39. CNTF(Ax15) bypasses diet-induced leptin resistance to reduce hypothalamic AMPK activity PMID: 16675525
    40. Results indicate that Sox10 is necessary and sufficient for regulating ciliary neurotrophic factor expression in the peripheral nervous system. PMID: 16684879
    41. Three-dimensional reconstructions of CNTF-immunoreactive axonal bulbar projections of these neurons revealed an ordered bilaterally symmetric pattern. PMID: 16999202
    42. CNTF/leukemia inhibitory factor signaling pathway acts via regulation of nitric oxide production to modulate developmental programmed cell death of postmitotic rod precursor cells. PMID: 17054938
    43. Ciliary neurotrophic factor (CNTF) elicits robust but primarily intraterminal sprouting confined to the endplate region and contributing little to synapse formation. PMID: 17614103
    44. Co-administration of ciliary neurotrophic factor with its soluble receptor protects against neuronal death and enhances neurite outgrowth. PMID: 18086669
    45. Continuous expression of striatal ciliary neurotrophic factor is detrimental to Huntington's and wild-type mice, causing abnormal behavior in wild-type mice. PMID: 18293418
    46. Ciliary neurotrophic factor mediates dopamine D2 receptor-induced CNS neurogenesis in adult mice. PMID: 18305256
    47. In vivo and in vitro experiments implicate CNTF as an endogenous regulatory component of dopamine D2-receptor-dependent neurogenesis in the subventricular zone and the dentate gyrus of the hippocampus. (Review) PMID: 18524890
    48. Deletion of the mouse RegIIIbeta (Reg2) gene disrupts ciliary neurotrophic factor signaling and delays myelination of mouse cranial motor neurons PMID: 18678917
    49. Neurogenesis in the dentate gyrus depends on ciliary neurotrophic factor and signal transducer and activator of transcription 3 signaling. PMID: 19023034
    50. Data demonstrate that CNTF is a potent axon growth-promoting factor for mature retinal ganglion cells (RGCs), and that exogenously applied CNTF stimulates RGCs indirectly via a mechanism that depends on astrocyte-derived CNTF. PMID: 19332123

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    CNTF family
  • 组织特异性：
    Nervous system.
  • 数据库链接：

    KEGG: mmu:12803

    STRING: 10090.ENSMUSP00000108555

    UniGene: Mm.290924