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  4. Recombinant Mouse Cofilin-1 (Cfl1)

Recombinant Mouse Cofilin-1 (Cfl1)

  • 货号：
    CSB-YP005280MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005280MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005280MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005280MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005280MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cfl1; Cofilin-1; Cofilin; non-muscle isoform
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-166
  • 氨基酸序列
    ASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYTT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPENAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
  • 基因功能参考文献：
    1. this study reports that 5'UTR of cofilin mRNA contains an internal ribosome entry site (IRES) element, and cofilin is predominantly translated by IRES-mediated mechanism in neurons. PMID: 29440227
    2. These findings suggest a causal relationship between increased Rac1-cofilin signaling, synaptic defects, and impaired sensory processing in fragile X syndrome (FXS) and uncover a previously unappreciated role for impaired Rac1-cofilin signaling in the aberrant spine morphology and spine density associated with FXS. PMID: 29114038
    3. Our work unraveled a novel function for cofilin1-dependent actin dynamics in mitochondrial fission, and identified cofilin1 as a negative regulator of mitochondrial DRP1 activity PMID: 28981113
    4. These findings suggest that the patterning of podosomes into a sealing zone involves the dynamic interaction between cofilin, CTTN, and the microtubule + ends. PMID: 27064822
    5. the Rap1-cofilin-1 pathway coordinates actin and microtubule organization at the immune synapse. PMID: 28167682
    6. VEGFA induced phosphorylation of Pak1 and its effector cofilin in a manner that was dependent on time as well as p38MAPKbeta. PMID: 26857814
    7. Data show that the primary effect of costimulation blockade was to decrease recruitment of the activator of actin nucleation WAVE2 (Wiskott-Aldrich syndrome protein family member protein 2) and cofilin (actin-severing protein) to F-actin. PMID: 27095595
    8. These results strongly support the active role of cofilin in ischemia-induced neuronal degeneration and apoptosis. PMID: 25526862
    9. aberrant cofilin phosphorylation that induces actin polymerization might be a consequence of actin disassembly induced by the absence of Rac1. PMID: 26496994
    10. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
    11. reelin and cofilin cooperate in controlling cytoskeletal dynamics during neuronal migration. PMID: 26893343
    12. The data of this study identify overlapping functions for ADF and n-cofilin in presynaptic physiology and vesicle in mice. trafficking. PMID: 24770705
    13. Actin depolymerizing factor/cofilin double mutant mice exhibited hyperlocomotion, impulsivity, and impaired working memory. Hyperlocomotion and impulsive behavior were reversed by methylphenidate. PMID: 24768258
    14. On the basal side of the neural plate, loss of CFL1 has the opposite effect on myosin: excess F-actin and myosin accumulate and the ectopic myosin light chain is phosphorylated. PMID: 25742799
    15. LIMK1 and Cofilin phosphorylation depends on PKA and is essential for mouse sperm acrosomal exocytosis PMID: 26169470
    16. prion protein is a co-receptor mediating neuronal cofilin-actin rod formation induced by beta-amyloid and proinflammatory cytokines PMID: 25426519
    17. The principal role of cofilin in lamellipodia at steady state is to break down F-actin, control filament turnover, and regulate the rate of retrograde flow. PMID: 23676663
    18. Subsequent analysis by MALDI-MS identified cofilin as one of the most prominently upregulated cytoskeletal protein in pulmonary hypertension PMID: 23161571
    19. analysis of cofilin activation during podosome belt formation in osteoclasts PMID: 23049890
    20. Cofilin and Vangl2 cooperate in the initiation of planar cell polarity in the mouse embryo. PMID: 23406901
    21. forebrain-specific inactivation of Cfl1 decreased anxiety in knockout mice. PMID: 23055942
    22. The inactive phosphorylated state of cofilin is mediated through Syk tyrosine kinase and mediates myelin phagocytosis. PMID: 22776089
    23. the SRF-cofilin-actin signaling axis modulates neuronal mitochondrial function PMID: 22927399
    24. The ablation of n-cofilin proteins did not change cell death progression. PMID: 22139132
    25. Macrophage migration, cell polarization and antigen presentation to T-cells require n-cofilin mediated F-actin remodelling. PMID: 22558315
    26. studies demonstrate unique functions of beta-arrestin-2 in NMDAR-mediated dendritic spine and synapse plasticity through spatial control over cofilin activation PMID: 22308427
    27. n-cofilin can compensate for the loss of ADF in excitatory synapses. ADF and n-cofilin cooperate in controlling synaptic actin content PMID: 22046357
    28. Cofilin activation is required for CD95-stimulated formation of membrane protrusions and increased tumour cell invasion. PMID: 21760611
    29. Cofilin cooperates with fascin to disassemble filopodial actin filaments PMID: 21940796
    30. Leukotrienes target F-actin/cofilin-1 to enhance alveolar macrophage anti-fungal activity. PMID: 21715328
    31. microRNAs 103 and 107 repress translation of cofilin PMID: 21179570
    32. Results indicate that ADF activity, provided by either cofilin1 or destrin, is essential in UB epithelial cells for normal growth and branching. PMID: 21060807
    33. Data suggest that in wild-type animals this aberrant neuronal medial migration towards the central canal is prevented by Reelin-induced cytoskeletal stabilization, brought about by phosphorylation of cofilin. PMID: 21039973
    34. Silencing of cofilin-1 led to an Accumulation of F-actin fibers and significantly decreased podocyte migration. PMID: 20838616
    35. Findings show that ADF/n-cofilin-null MKs develop significantly fewer proplatelets. PMID: 20530287
    36. Cofilin-1 is necessary for maintenance of normal podocyte architecture and for actin structural changes that occur during induction and recovery from podocyte injury. PMID: 20472933
    37. Data show that HUNK reconstitution in basal breast cancer cell lines prevented protein phosphatase 2-A (PP2A from binding to CFL-1. PMID: 20133759
    38. cofilin1 plays an important role in cortical cytoplasmic organization during embryo compaction. PMID: 19751773
    39. role of Cofilin-1 in actin dynamics PMID: 11809832
    40. These results show that cofilin plays an essential role in the control of phagocyte function through regulation of actin filament dynamics. PMID: 12297504
    41. Data suggest that ADF and cfl1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for cytokinesis and cell movement. PMID: 15548599
    42. a novel pathway emanating from the TGF-beta type I receptor and leading to regulation of actin assembly, via the kinase LIMK2. PMID: 15647284
    43. complete lack of neural tube closure in n-cofilin mutant embryos PMID: 15649475
    44. Results suggest that cofilin and integrin alphavbeta3 together regulate K1735 melanoma cell invasion. PMID: 16337627
    45. These results indicate that cofilin severs the actin filament by a mechanism of allosteric and cooperative destabilization. PMID: 17134718
    46. Caspase-11 interacts physically and functionally with Aip1 to promote cofilin-mediated actin depolymerization. PMID: 17293856
    47. Loss of n-cofilin impairs radial migration, resulting in the lack of intermediate cortical layers. PMID: 17875668
    48. Differential expression of RhoGDI-1, cofilin 1 and tropomyosin 2 in calpain-deficient fibroblasts compared with wild-type fibroblasts PMID: 18076376

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  • 亚细胞定位：
    Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium.
  • 蛋白家族：
    Actin-binding proteins ADF family
  • 组织特异性：
    Widely distributed in various tissues. Not found in skeletal muscle.
  • 数据库链接：

    KEGG: mmu:12631

    STRING: 10090.ENSMUSP00000112259

    UniGene: Mm.329655