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  4. Recombinant Mouse Collagen alpha-1 (X) chain (Col10a1)

Recombinant Mouse Collagen alpha-1 (X) chain (Col10a1)

  • 中文名称：
    Recombinant Mouse Collagen alpha-1(X) chain(Col10a1),Yeast
  • 货号：
    CSB-YP005715MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-1(X) chain(Col10a1),Yeast
  • 货号：
    CSB-EP005715MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-1(X) chain(Col10a1),Yeast
  • 货号：
    CSB-EP005715MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-1(X) chain(Col10a1),Yeast
  • 货号：
    CSB-BP005715MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-1(X) chain(Col10a1),Yeast
  • 货号：
    CSB-MP005715MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Col10a1Collagen alpha-1(X) chain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-680
  • 氨基酸序列
    MF YAERYQTPTG IKGPLASPKT QYFIPYAIKS KGIPVRGEQG IPGPPGPTGP RGHPGPSGPP GKPGYGSPGL QGEPGLPGPP GISATGKPGL PGPPGKPGER GPYGHKGDIG PAGLPGPRGP PGPPGIPGPA GISVPGKPGQ QGLTGAPGPR GFPGEKGAQG APGVNGRKGE TGYGSPGRPG ERGLPGPQGP IGPPGPSGVG RRGENGFPGQ PGIKGDRGFP GEMGPSGPPG PQGPPGKQGR EGIGKPGAIG SPGQPGIPGE KGHPGAPGIA GPPGAPGFGK QGLPGLRGQR GPAGLPGAPG AKGERGPAGH PGEPGLPGSP GNMGPQGPKG IPGNHGIPGA KGEIGLVGPA GPPGARGARG PPGLDGKTGY PGEPGLNGPK GNPGLPGQKG DPGVGGTPGL RGPVGPVGAK GVPGHNGEAG PRGEPGIPGT RGPTGPPGVP GFPGSKGDPG NPGAPGPAGI ATKGLNGPTG PPGPPGPRGH SGEPGLPGPP GPPGPPGQAV MPDGFIKAGQ RPRLSGMPLV SANHGVTGMP VSAFTVILSK AYPAVGAPIP FDEILYNRQQ HYDPRSGIFT CKIPGIYYFS YHVHVKGTHV WVGLYKNGTP TMYTYDEYSK GYLDQASGSA IMELTENDQV WLQLPNAESN GLYSSEYVHS SFSGFLVAPM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • 基因功能参考文献：
    1. Results indicate that Cox-2 promotes Col10a1 expression and chondrocyte hypertrophy in vitro. PMID: 27121205
    2. In a model of instability-induced osteoarthritis, Col X was overexpressed in articular chondrocytes and osteophyte-forming cells in LOX-1(+)/(+) mice and was significantly downregulated in articular chondrocytes and osteophyte-forming cells in LOX-1(-)/(-) mice. PMID: 26901593
    3. Col10 expression was seen in the hypertrophic zone at and below the tidemark of the mandibular condyle cartilage. PMID: 26362410
    4. These data support a Cox-2-containing, Runx2-centered Col10a1 regulatory mechanism, during chondrocyte hypertrophic differentiation. PMID: 25321476
    5. these results show that increased Col10a1 expression in Phex-deficient osteoblasts is not a major cause of the XLHR phenotype, which was an important issue to address based on the previous findings PMID: 24269824
    6. Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes. PMID: 23441228
    7. Annexins and collagen X may rather fulfill functions in growth plate cartilage not directly linked to the mineralization process. PMID: 22692895
    8. SOX9 directly represses Col10a1 expression in immature/proliferating chondrocytes of the growth plate PMID: 22072985
    9. Studies indicate that Col10a1 is highly expressed in hypertrophic chondrocytes and commonly used as a gene marker for this cell population. PMID: 21328521
    10. cartilaginous regeneration is due to the expression of chondrogenic factors, such as Sox 9 and type II and X collagens PMID: 20403959
    11. endochondral ossification-specific collagen X contributes to the establishment of a hematopoietic niche at the chondro-osseous junction PMID: 20209091
    12. demonstration of sleketal defects and altered marrow and hematopoiesis in all Tg mice with dominant interference mutations for collagen X PMID: 12057907
    13. The 4.6 kb promoter is able to drive specific expression of Col10a1 in hypertrophic cartilage. PMID: 15464363
    14. the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation PMID: 16806867
    15. TSG is an important modulator of BMP-regulated induced collagen II and X expression and cartilage development and chondrocyte differentiation PMID: 16905550
    16. nonsense/frame-shift mutations can elicit a gain-of-function effect, affecting chondrocyte differentiation in the growth plate PMID: 17403716
    17. Indian hedgehog, expressed in pre-hypertrophic cells and collagen type X, expressed in hypertrophic chondrocytes, were both significantly inhibited after incubation with isoproterenol PMID: 18059015
    18. These data suggest that the 3' UTR participates in collagen X last-exon mRNA decay and that overall 3' UTR configuration, rather than specific linear-sequence motifs, may be important in specifying decay of Col10a1 mRNA containing nonsense mutations PMID: 18304492
    19. Results show that in Smad3 gene knock-out mice the protein positive staining of type II collagen, aggrecan, and TGF-beta1 in the disc decreased, while that of type X collagen increased. PMID: 19478656
    20. Mice expressing the collagen X mutation had shortened limbs and an expanded hypertrophic zone. PMID: 19834559

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 数据库链接：

    KEGG: mmu:12813

    STRING: 10090.ENSMUSP00000101150

    UniGene: Mm.443177