Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Collagen alpha-2 (VIII) chain (Col8a2)

Recombinant Mouse Collagen alpha-2 (VIII) chain (Col8a2)

  • 中文名称：
    Recombinant Mouse Collagen alpha-2(VIII) chain(Col8a2),Yeast
  • 货号：
    CSB-YP005756MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-2(VIII) chain(Col8a2),Yeast
  • 货号：
    CSB-EP005756MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-2(VIII) chain(Col8a2),Yeast
  • 货号：
    CSB-EP005756MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-2(VIII) chain(Col8a2),Yeast
  • 货号：
    CSB-BP005756MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Collagen alpha-2(VIII) chain(Col8a2),Yeast
  • 货号：
    CSB-MP005756MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Col8a2
  • Uniprot No.：
  • 别名：
    Col8a2Collagen alpha-2(VIII) chain; Endothelial collagen
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-699
  • 氨基酸序列
    GGGAGG AAGYAPVKYV QPMQKGPVGP PFREGKGQYL EMPLPMLPMD LKGEPGPPGK PGPRGPPGPP GFPGKPGTGK PGVHGQPGPA GPPGFSRMGK AGPPGLPGKV GPPGQPGLRG EPGIRGDQGL RGPPGPPGLP GPSGITVPGK PGAQGAPGPP GFRGEPGPQG EPGPRGDRGL KGDNGVGQPG LPGAPGQAGA PGPPGLPGPA GLGKPGLDGI PGAPGDKGDS GPPGVPGSRG EPGAVGPKGP PGVDGVGIPG AAGVPGPQGP VGAKGEPGLR GPPGLIGPVG YGMPGKPGPK GDRGPVGAPG LLGDRGEPGE DGKPGEQGPQ GLGGPPGLPG SAGLPGRRGP PGSKGEVGPG GPPGVPGIRG DQGPNGLAGK PGLPGERGLP GAHGPPGPTG PKGEPGFTGR PGGPGVAGAL GQKGDLGLPG QPGLRGPSGI PGLQGPAGPI GPQGLPGLKG EPGLPGPPGE GKVGEPGSAG PTGPPGVPGS PGLTGPPGPP GPPGPPGAPG ALDETGIAGL HLPNGGVEGA VLGKGGKPQF GLGELSAHAT PAFTAVLTSP FPASGMPVRF DRTLYNGHSG YNPATGIFTC PVGGVYYFAY HVHVKGTNVW VALYKNNVPA TYTYDEYKKG YLDQASGGAV LQLRPNDQVW VQMPSDQANG LYSTEYIHSS FSGFLLCPT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.
  • 基因功能参考文献：
    1. Overexpression of the Col8a2 gene in mouse and human Fuchs endothelial corneal dystrophy endothelial cells suggested a role for altered autophagy in this disease. PMID: 23422828
    2. in contrast to diabetic wild-type mice EMT-like changes were attenuated in diabetic Col8alpha1/alpha2-KO mice, indicating that collagen VIII may be 1 of the major inducers of epithelial-to-mesenchymal transition-like changes in kidneys of diabetic wild-type mice PMID: 22759394
    3. study confirms the Q455K substitution in the COL8A2 gene as being sufficient to cause FECD in the first mouse model of this disease and supports the role of the UPR and UPR-associated apoptosis in the pathogenesis of FECD caused by COL8A2 mutations PMID: 22002996
    4. Type VIII collagen significantly modulates the effect of TGF-beta1 on mesangial cells and may therefore play a role in the pathogenesis of diabetic nephropathy. PMID: 21372207
    5. Smooth muscle cells can modify the matrix microenvironment by producing type VIII collagen, using it to overlay type I collagen, and generating a substrate favorable for migration PMID: 16269661
    6. Lack of type VIII collagen confers renoprotection in diabetic nephropathy in a knockout mouse model. PMID: 19401424
    7. The mouse mutant Aca23 described here offers the first point mutation of the Col8a2 gene and suggests that a functional collagen VIII alpha2 gene is essential for the correct assembly of the Descemet's membrane and for corneal stability. PMID: 19578028

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane.
  • 组织特异性：
    In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells.
  • 数据库链接：

    KEGG: mmu:329941

    STRING: 10090.ENSMUSP00000070270

    UniGene: Mm.296327