Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Complexin-1 (Cplx1)

Recombinant Mouse Complexin-1 (Cplx1)

  • 货号：
    CSB-YP005893MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005893MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005893MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005893MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005893MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cplx1Complexin-1; 921-S; Complexin I; CPX I; Synaphin-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-134
  • 氨基酸序列
    MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEPE EEDESILDTV IKYLPGPLQD MFKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
  • 基因功能参考文献：
    1. The response of CPLX1 and Foxp1 levels to SNCA deficiency supports the notion that these factors are regulated by altered physiological function of alpha-synuclein. PMID: 25112678
    2. In the absence of Cplx1, synaptic vesicles remain unstable and prone to premature fusion. PMID: 26019341
    3. Cplx 1 and 2 play a role in facilitating vesicle priming, and also lead to the new hypothesis that Cplxs may synchronize vesicle release by promoting coupling between secretory vesicles and calcium channels. PMID: 23401610
    4. Complexin-1 carboxyl-terminal domain binds lipids through a novel protein motif by targeting complexin-1 to synaptic vesicles. PMID: 23352168
    5. the ataxia in Cplx1(-/-) mice is likely to be due to pathological changes in both cerebellum and thalamus PMID: 22393426
    6. These results suggest a complexin-dependent molecular mechanism for regulating AMPAR delivery to synapses PMID: 22284181
    7. This study demonistrated that relatively large C-terminal complexin-1 sequence acts in priming and clamping synaptic exocytosis and demonstrate that the clamping function PMID: 22357870
    8. The study uncovers an interaction between the complexin-1 N terminus and the SNARE complex C terminus, and shows that disrupting this interaction abolishes the facilitatory function of complexins in mouse neurons. PMID: 20400951
    9. We studied the mRNA distribution of CPLXI and CPLXII in mouse brain. We show that while CPLXs are expressed in distinct cell populations they do not segregate with particular neurotransmitters or different classes of transmitter action PMID: 15121237
    10. CPX I plays a critical role in beta-cells in the control of the stimulated-exocytosis of insulin. PMID: 15126625
    11. Here we propose that complexin binding activates SNARE complexes into a metastable state and that Ca(2+) binding to synaptotagmin 1 triggers fast exocytosis by displacing complexin from metastable SNARE complexes. PMID: 16990140
    12. Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis. PMID: 17133603
    13. early behavioural development of Cplx1(-/-) mice showed marked abnormalities PMID: 17188502
    14. Complexin 1 knockout mice have pronounced deficits in social behaviours in addition to the severe motor and exploratory deficits. PMID: 17652102
    15. complexin I functions in exocytosis in two related but morphologically distinct secretory processes. PMID: 17692307
    16. Results indicate that distinct functional domains of copmplexin I differentially regulate synaptic exocytosis, and that CplxI carries out a crucial role in fine-tuning Ca2+-triggered fast neurotransmitter release. PMID: 17828276
    17. Data show that Cplx1 in the locus Rgcs1 region is highly expressed in the ganglion cell layer and optic nerve head. PMID: 18671875
    18. study shows in neuronal synapses, complexin simultaneously suppressed spontaneous membrane fusion & activated fast calcium ion-evoked fusion; complexin probably activates & clamps force transfer from assembled trans-SNARE complexes onto fusing membranes PMID: 19164751
    19. Complexin 1 is required for faithful processing of sound onset and offset in the cochlear nucleus. PMID: 19553439

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytosol. Perikaryon. Cell junction, synapse, presynapse.
  • 蛋白家族：
    Complexin/synaphin family
  • 组织特异性：
    Nervous system, and pancreatic islet cells. Present in many brain regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level).
  • 数据库链接：

    KEGG: mmu:12889

    STRING: 10090.ENSMUSP00000038502

    UniGene: Mm.5195