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  4. Recombinant Mouse Condensin-2 complex subunit H2 (Ncaph2)

Recombinant Mouse Condensin-2 complex subunit H2 (Ncaph2)

  • 货号：
    CSB-YP806899MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806899MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806899MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806899MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806899MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ncaph2
  • Uniprot No.：
  • 别名：
    Ncaph2; D15Ertd785eCondensin-2 complex subunit H2; Kleisin-beta; Non-SMC condensin II complex subunit H2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-607
  • 氨基酸序列
    MEDVEVRFAH LLQPIRDLTK NWEVDVAAQL GEYLEELDQI CISFDEGKTT MNFIEAALLI QGSACVYSKK VEYLYSLVYQ ALDFISGKRR AKQLSLVQED GSKKTVNSET PCETENEFLS LDDFPDSRAN VDLKNDQASS ELLIIPLLPM ALVAPDEVEK NSSPLYSCQG DILASRKDFR MNTCMPNPRG CFMLDPVGMC PVEPVVPVEP YPMSRSQKDP EDAEEQPMEV SRNGSPVPVP DISQEPDGPA LSGGEEDAED GAEPLEVALE PAEPRTSQQS AILPRRYMLR ERQGAPEPAS RLQETPDPWQ SLDPFDSLES KVFQKGKPYS VPPGVEEAPG QKRKRKGATK LQDFHKWYLD AYAEHPDGRR ARRKGPTFAD MEVLYWKHVK EQLETLQKLR RRKINERWLP GAKQDLWPTE EDRLEESLED LGVADDFLEP EEYVEEPAGV MPEEAADLDA EAMPESLRYE ELVRRNVELF IATSQKFIQE TELSQRIRDW EDTIQPLLQE QEQHVPFDIH IYGDQLASRF PQLNEWCPFS ELVAGQPAFE VCRSMLASLQ LANDYTVEIT QQPGLEAAVD TMSLRLLTHQ RAHTRFQTYA APSMAQP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of chromatin bridges at anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size. Seems to have lineage-specific role in T-cell development.
  • 基因功能参考文献：
    1. mice inheriting missense mutations in a condensin II subunit (Caph2(nes)) develop T-cell lymphoma PMID: 27737961
    2. Condensins I and II have overlapping and non-overlapping functions in Neural stem cells. PMID: 25474630
    3. condensin II (beta-kleisin) has an essential role during meiosis I in mouse oocytes and its function overlaps with that of condensin I (gamma-kleisin), which is otherwise redundant PMID: 25961503
    4. We confirm that Ncaph2 can generate three different N-termini, all encoded by exon 1, one of which is translated from an alternative reading frame. PMID: 22333158
    5. define a unique lineage-specific requirement for kleisin beta in mammalian T cell differentiation PMID: 17640884

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  • 相关疾病：
    Defects in Ncaph2 are the cause of the nessy phenotype which is characterized by a specific defect in T-cell development. Nessy thymuses are smaller, with corticomedullary junctions less well defined, and cortical cells sparser than in wild-type. The thymocyte defect is typified by an increased proportion of CD4-CD8- DN T-cell progenitors. Only thymocyte differentiation is affected in Nessy mice and not cell differentiation.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CND2 H2 (condensin-2 subunit 2) family
  • 数据库链接：

    KEGG: mmu:52683

    STRING: 10090.ENSMUSP00000074139

    UniGene: Mm.143167